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Production of Cellulases by Phanerochaete sp. Using Empty Fruit Bunches of Palm (EFB) as Substrate: Optimization and Scale-Up of Process in Bubble Column and Stirred Tank Bioreactors (STR)

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Abstract

The goal of this study was to screen microorganisms that are potential producers of cellulases, determine optimal conditions for production of these enzymes, and scale up the process, using empty fruit bunches (EFB) as a substrate. Strains of Trichoderma sp., Aspergillus niger, Phanerochaete sp., Ganoderma sp. and Lentinus sp. were evaluated for their ability to produce cellulolytic enzyme complexes under submerged with suspended solids fermentation (SSdF). Among these microorganisms, Phanerochaete sp. (PH-HD), which is already known to be a good producer of lignolytic enzymes, presented the highest capacity for cellulase production. The highest level of cellulase production by Phanerochaete sp. was observed on the 4th day of fermentation at 28 °C and pH 5.7, reaching a value of FPase activity of 364 IU L−1 using 15 g L−1 of EFB as a carbon source, 2 g L−1 of urea as a nitrogen source, 4 g L−1 of KH2PO4, and microcrystalline cellulose Avicel® PH-101 as an inducer (2 g L−1). This study shows the great potential of Phanerochaete sp. for producing cellulolytic enzymes, as well as the feasibility of using EFB under suspended solids fermentation in the production process.

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Acknowledgments

This work was supported by the Co-Operative Project between Vale S.A. and UFPR intitled BIOPAL—Integrated Biorefineries to Sustainable Processing of Palm—Biological Route Subproject C1—Residues from palm as substrate to production of the Cellulolytc Enzimatic Complex.

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  1. Bioprocess Engineering and Biotechnology Department, Federal University of Parana, UFPR, Av. Cel. Fco. H dos Santos, 100, Curitiba, PR, 81531-990, Brazil

    Marcelo Adriano Corrêa Maceno, Luciana Porto de Souza Vandenberghe, Adenise Lorenci Woiciechowski, Carlos Ricardo Soccol & Michele Rigon Spier

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  1. Marcelo Adriano Corrêa Maceno
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Correspondence to Michele Rigon Spier.

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Maceno, M.A.C., de Souza Vandenberghe, L.P., Woiciechowski, A.L. et al. Production of Cellulases by Phanerochaete sp. Using Empty Fruit Bunches of Palm (EFB) as Substrate: Optimization and Scale-Up of Process in Bubble Column and Stirred Tank Bioreactors (STR). Waste Biomass Valor 7, 1327–1337 (2016). https://doi.org/10.1007/s12649-016-9503-7

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