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Morphologic and molecular analysis of Dicrocoelium dendriticum species from Iranian native sheep

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Abstract

Dicrocoeliosis is a worldwide parasitic disease of ruminants which affects the liver. In this current study, the phylogenic pattern of Dicrocoelium species in Iranian native sheep from three different geographical regions was investigated by investigating a 520 bp fragment of mitochondirial NAD1 gene. The analysis of the NAD1 oligo nucleotide sequences from 10 D. dendriticum (GenBank accession numbers: MG889399 to MG889408) revealed few non-significant differences, suggesting limited application for NAD1 gene as a biomarker for study of genetic variation in Dicrocoelium. The morphometrical study also showed a significant relationship for the several morphometric indices among the Dicrocoelium spp. isolates from different regions of Iran.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, PO Box 165, Urmia, Iran

    Laya Shamsi, Mousa Tavassoli & Soraya Naem

  2. Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Alireza Mahmoudian

  3. Agricultural Zoology Research Department, Iranian Research Institute of Plant Protection, Tehran, Iran

    Elham Ahmadi

Authors
  1. Laya Shamsi
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  2. Mousa Tavassoli
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  3. Soraya Naem
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  4. Alireza Mahmoudian
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  5. Elham Ahmadi
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Correspondence to Mousa Tavassoli.

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Shamsi, L., Tavassoli, M., Naem, S. et al. Morphologic and molecular analysis of Dicrocoelium dendriticum species from Iranian native sheep. J Parasit Dis 44, 441–446 (2020). https://doi.org/10.1007/s12639-020-01201-z

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  • DOI: https://doi.org/10.1007/s12639-020-01201-z

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