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Diagnotic value of some Fasciola gigantica antigens

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Abstract

The present study was aimed to select the specificity of antigens for Fasciola gigantica depending on its diagnostic utility and field applications. The tested antigens were coproantigen, excretory–secretory (ES) antigen and egg antigen. Coproantigen and Copro Hyperimmune serum were able to reflect the lowest level of cross-reaction with other tested F. gigantica antigens. By using SDS-PAGE, a structural homology was observed in F. gigantica ES and egg antigens. Intense cross reaction was observed between ES and egg antigens by ELISA technique even when there was no cross-reaction with coproantigen. The 27.6 kDa band proved to be the most specific in F. gigantica coproantigen and was different from the band at the same molecular weight by ES antigen. The results conclude that coproantigens show specific diagnostic ability for Fasciola and have low numbers of cross-reaction proteins reflecting its high specificity. Moreover, detection of coproantigen in faeces offers a new potential for diagnostics as compared to serum samples. This fact holds promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection.

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Authors and Affiliations

  1. Medical Division, Department of Complementary Medicine, National Research Centre, Cairo, Egypt

    Said Shalaby

  2. Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt

    Mohammad El-Bahy & Ali Hassan

  3. Veterinary Division, Department of Parasitology, National Research Centre, Cairo, Egypt

    Hatem Shalaby

  4. Department of Animal Science, Centre of Excellence, MJP Rohilkhand University, Bareilly, 243006, India

    Neelima Gupta

Authors
  1. Said Shalaby
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  2. Mohammad El-Bahy
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  3. Ali Hassan
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  4. Hatem Shalaby
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  5. Neelima Gupta
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Correspondence to Neelima Gupta.

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Shalaby, S., El-Bahy, M., Hassan, A. et al. Diagnotic value of some Fasciola gigantica antigens. J Parasit Dis 39, 479–486 (2015). https://doi.org/10.1007/s12639-013-0379-1

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  • DOI: https://doi.org/10.1007/s12639-013-0379-1

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