Skip to main content
SpringerLink
Log in

Natural trematode infection in liver of water buffalo (Bubalus bubalis): histopathological investigation

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

This study reports the infection of liver and bile ducts, carried out from November 2008 to April 2010, on 730 randomly selected water buffaloes—Bubalus bubalis, infected with the amphistome trematode parasite Explanatum explanatum (Creplin, 1847) Fukui, 1929. Macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. The predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. Histopathological study of 4 μm thick tissue sections cut adjacent to and through the site of attachment of individual worm and stained with hematoxylin and eosin revealed intense infiltration of inflammatory cells such as lymphocytes, macrophages, plasma cells, eosinophils as well as fibrocytes. This was associated with fibrosis and thickening of the bile ducts. Due to high level of prevalence and intensity of natural infection, amphistomiasis appears to be endemic in this geographical region and probably represent one of the most important animal health problems. It is hoped that the study may draw attention to the need for educating farmers, regarding the economic importance of infection of these amphistome parasites and also for the development of control strategies to prevent the spread of infection to ruminants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ahmedullah F, Akbor M, Haider MG, Hossain MM, Khan MAHNA, Hossain MI, Shanta IS (2007) Pathological investigation of liver of the slaughtered buffaloes in Barisal district. Bangladesh J Vet Med 5(1-2):81–85

    Google Scholar 

  • Bassily S, Iskandar M, Youssef FG, el Masry N, Bowden M (1989) Sonography in diagnosis of fascioliasis. Lancet 1:1270–1271

    Article  PubMed  CAS  Google Scholar 

  • Hafeez MD, Rao BV (1989) A brief note on gross and histopathological lesions in buffaloes infected with biliary amphistomiasis. Indian J Anim Health 28:83–84

    Google Scholar 

  • Haque M, Siddiqi AH (1978) Histopathology of gastrointestinal trematode parasites of pig and man. Indian J Parasitol 2(2):97–99

    Google Scholar 

  • Makay O, Gurcu B, Caliskan C, Nart D, Tuncyurek M, Korkut M (2007) Ectopic fascioliasis mimicking a colon tumor. World J Gastroenterol 13(18):2633–2635

    PubMed  Google Scholar 

  • Maria Teresa C, Deborah C, Vincenzo DM, Miriam R, Vincenzo A, Amedeo T, Lazzara A, Amedeo S, Elepnora SF, Bruno D, Franco G (2009) Natural trematode infection in feral Nebrodi Black pigs: pathological investigations. Vet Parasitol 159:37–42

    Article  Google Scholar 

  • Mas-Coma S, Bargues MD, Valero MA (2005) Fascioliasis and other plant-borne trematode zoonoses. Invited review. Int J Parasitol 32:1255–1278

    Article  Google Scholar 

  • Menard A, Agoulon A, L’Hostin M, Rondelaud D, Collard S, Chauvin A (2001) Myocastor coypus as a reservoir host of Fasciola hepatica in France. Vet Res 32:499–508

    Article  PubMed  CAS  Google Scholar 

  • Nansen P, Anderson S, Harmer E, Riising HJ (1972) Experimental fascioliasis in the pig. Exp Parasitol 31:247–254

    Article  PubMed  CAS  Google Scholar 

  • Pile E, Santos JAA, Coelho B, Serra-Freire NM (2000) Ocorrencia fascioliasis humana no municipio de Volta Redoda. RJ, Brasil. Rev Saude Publica 34:413–414

    Article  PubMed  CAS  Google Scholar 

  • Serra-Freire NM (1995) Fasciolose hepatica. Hor Vet 1:13–18

    Google Scholar 

  • Upadhayay AN, Prasad B, Jha GJ, Sahai BN (1987) Histochemistry and histopathology of liver of buffaloes naturally infected with Gigantocotyle explanatum. Indian J Anim Sci 57:180–185

    Google Scholar 

  • Wiedosari E, Graydon R, Copeman DB (1991) Comparative pathological study of hepatic changes induced by Fasciola gigantica and Gigantocotyle explanatum in Javanese thin-tailed sheep. J Comp Pathol 105(2):147–155

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The study was supported by the project grant No. 37/518/2009 (SR), from the University Grants Commission, Government of India, New Delhi. The authors are also grateful to Prof. Wasim Ahmad, Department of Zoology, Aligarh Muslim University, Aligarh, for photomicrograph facilities.

Author information

Authors and Affiliations

  1. Parasitology Research Lab, Department of Zoology, Patna University, Patna, Bihar, 800005, India

    Masoodul Haque & Chandra Mohan

  2. Department of Pathology, Darbhanga Medical College, Laheriasarai, Darbhanga, Bihar, 846003, India

    Imtiaz Ahmad

Authors
  1. Masoodul Haque
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chandra Mohan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Imtiaz Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masoodul Haque.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haque, M., Mohan, C. & Ahmad, I. Natural trematode infection in liver of water buffalo (Bubalus bubalis): histopathological investigation. J Parasit Dis 35, 50–53 (2011). https://doi.org/10.1007/s12639-011-0022-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-011-0022-y

Keywords

Search

Navigation