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Nostoc calcicola Immobilized in Silica-coated Calcium Alginate and Silica Gel for Applications in Heavy Metal Biosorption

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Abstract

The present study reports the preparation and characterization of silica-based immobilization matrices for the purpose of metal accumulation using immobilized cyanobacterium Nostoc calcicola. Silica gel was prepared using aqueous sodium silicate and colloidal silica. Calcium alginate (CAG) beads were coated with silica using sodium silicate solutions. Microscopy observations and TTC tests confirmed that the immobilized cells were intact and viable. Ultrastructural studies with electron microscopy revealed a membrane thickness of approximately 10 μm around the CAG and the silica gel to be of mesoporous nature. BET surface area of silica gel-immobilized N. calcicola was 160 m2 g−1. The porous volume and average pore diameter were 0.40 cm3 g−1 and ca. 100 Å, respectively, as calculated using the BJH model. Studies on silica-coated calcium alginate immobilized cells showed that these were superior to the uncoated CAG beads in terms of mechanical strength and metal accumulation. The silica matrices were found to be stable for repeated cycles of metal removal and with commonly used eluants for desorption processes. These matrices have potential applications in immobilization of industrially important biocatalysts.

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Authors and Affiliations

  1. Centre for Biotechnology, Biological Sciences Group, Birla Institute of Technology and Science, Pilani, Rajasthan, 333031, India

    Subramanian Ramachandran, Pankaj Kumar Jain & Sanjay Kumar Verma

  2. UPMC-Paris 6, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcelin Berthelot, 75231, Paris cedex 05, France

    Thibaud Coradin

Authors
  1. Subramanian Ramachandran
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  2. Thibaud Coradin
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  3. Pankaj Kumar Jain
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  4. Sanjay Kumar Verma
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Correspondence to Sanjay Kumar Verma.

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Ramachandran, S., Coradin, T., Jain, P.K. et al. Nostoc calcicola Immobilized in Silica-coated Calcium Alginate and Silica Gel for Applications in Heavy Metal Biosorption. Silicon 1, 215–223 (2009). https://doi.org/10.1007/s12633-009-9032-0

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  • DOI: https://doi.org/10.1007/s12633-009-9032-0

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