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Assessing the Potential Biological Activities of Postbiotics Derived from Saccharomyces cerevisiae: An In Vitro Study

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Abstract

A new biotherapeutic strategy involves the use of microbial bioactive substances (postbiotics) that exhibit optimum compatibility and intimate contact with the immune system of the host. This study was aimed at investigating the potential biological activities of postbiotics derived from Saccharomyces cerevisiae (PTCC 5269) (PSC) under in vitro circumstances. Based on the outcomes, the synthesized PSC possessing a high level of phenolic (102.46 ± 0.25 mg GAE/g) and flavonoid (19.87 ± 75.32 mg QE/g) content demonstrated significant radical scavenging activity (87.34 ± 0.56%); antibacterial action towards Listeria monocytogenes, Streptococcus mutans, Salmonella typhi, and Escherichia coli (in order of effectiveness) in both in vitro and food models (whole milk and ground meat); probiotics' growth-promoting activity in the fermentation medium; α-glucosidase enzyme-inhibiting and cholesterol-lowering properties in a concentration- and pH-dependent manner; reduction in the cell viability (with the significant IC50 values of 34.27 and 23.58 μg/mL after 24 and 48 h, respectively); suppressed the initial (G0/G1) phase of the cell's division; induced apoptosis; and increased the expression of PTEN gene, while the IkB, RelA, and Bcl-XL genes indicated diminished expression in treated SW480 cancer cells. These multiple health-promoting functions of PSC can be extended to medical, biomedical, and food scopes, as novel biotherapeutic approaches, in order to design efficient and optimized functional food formulations or/and supplementary medications to use as adjuvant agents for preventing or/and treating chronic/acute disorders.

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Data Availability

The data presented in this study are available on request from the corresponding author.

Abbreviations

PSC:

Postbiotics derived from Saccharomyces cerevisiae

QS:

Quorum sensing

YPD:

Yeast peptone dextrose

YMB:

Yeast malt broth

ABTS:

2,2′-Azino-bis [3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt

MHA:

Mueller Hinton agar

MHB:

Mueller Hinton broth

SDA:

Sabouraud dextrose agar

MSA:

Mannitol salt agar

EMB:

Eosin methylene blue

PCA:

Plate count agar

BHI:

Brain heart infusion

LB:

Luria Bertani

CT-SMAC:

Cefixime tellurite sorbitol MacConkey

CFS:

Cell-free supernatant

GAE:

Gallic acid equivalent

QE:

Quercetin equivalent

DDA:

Disc diffusion agar

MIC:

Minimum inhibitory concentration

MBC:

Minimum bactericidal concentration

WDA:

Well diffusion agar

MEC:

Minimal effective concentration

pNPG:

4-Nitrophenly α-D-glucopyranoside

5-FU:

5–Fluorouracil

PBS:

Phosphate-buffered saline

PI:

Propidium iodide

ANOVA:

One-way analysis of variance

ROS:

Reactive oxygen species

AFB1 :

Aflatoxin B1

CDC:

Centers for Disease Control and Prevention

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Acknowledgements

The authors would like to express their thanks to the Toxicology Research Center, Medical Basic Sciences Research Institute of Ahvaz Jundishapur University of Medical Sciences for the financial support of this study.

Funding

This research was funded by the Toxicology Research Center, Medical Basic Sciences Research Institute of Ahvaz Jundishapur University of Medical Sciences, grant number TRC-0059.

Author information

Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Nutrition Science and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hedayat Hosseini & Amin Abbasi

  2. Toxicology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Sahar Sabahi

  3. Department of Nutrition, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Sahar Sabahi

  4. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Sousan Akrami

  5. Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Arshid Yousefi-Avarvand

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  1. Hedayat Hosseini
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  2. Amin Abbasi
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Contributions

All of the authors contributed to the conception and design of the study and revised the manuscript. AA and SA conducted all the experiments and statistical analysis. AA, SS, and AYA drafted the first version of the manuscript. HH and SS reviewed the draft manuscript, and all the authors revised the final version of the manuscript.

Corresponding author

Correspondence to Sahar Sabahi.

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Ethics Approval and Consent to Participate

This study was approved by the ethics committee of the research and technology deputy of Ahvaz Jundishapur University of Medical Sciences (IR.AJUMS.REC.1400.609). All methods were carried out in accordance with relevant guidelines and regulations.

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Not applicable.

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Hosseini, H., Abbasi, A., Sabahi, S. et al. Assessing the Potential Biological Activities of Postbiotics Derived from Saccharomyces cerevisiae: An In Vitro Study. Probiotics & Antimicro. Prot. (2023). https://doi.org/10.1007/s12602-023-10117-y

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