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Development of a Murine Model with Optimal Routes for Bacterial Infection and Treatment, as Determined with Bioluminescent Imaging in C57BL/6 Mice

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Abstract

Mice intragastrically infected with Listeria monocytogenes EGDe and Staphylococcus aureus Xen 36 showed no visible signs of infection over 48 h. However, high numbers (6.2 × 105 cfu/mg feces) of S. aureus Xen 36 were detected 4 h, and 3.3 × 105 cfu/mg feces of L. monocytogenes EGDe 8 h, after administration. Mice intraperitoneally infected with S. aureus Xen 36 (1 × 107 cfu) developed infection immediately after administration and for at least the following 48 h. Injection with higher cell numbers of S. aureus Xen 36 (2 × 108 cfu) resulted in more intense bioluminescence (infection) of the peritoneal cavity. Injection of S. aureus Xen 36 in the tail and penile veins resulted in localized tissue infection for the first 120 h. Injection of S. aureus Xen 36 into the thigh produced a faint bioluminescent signal for 15 min. Nisin F injected into the peritoneal cavity at the same area of infection led to an immediate statistically significant decrease in infection (from 2 × 106 p/s/cm2/sr to 3 × 105 p/s/cm2/sr) within 2 h. Similar results were recorded when nisin F was injected subcutaneously. Intraperitoneal administration is an optimal administration route for bacterial infection and treatment with antimicrobial peptides.

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Acknowledgments

The authors thank the National Research Foundation (NRF), South Africa, for funding.

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Authors and Affiliations

  1. Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa

    Anneke M. Brand, Michele de Kwaadsteniet & Leon M. T. Dicks

  2. Department of Physiological Sciences, University of Stellenbosch, Stellenbosch, 7600, South Africa

    Rob Smith

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  1. Anneke M. Brand
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  2. Rob Smith
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  3. Michele de Kwaadsteniet
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  4. Leon M. T. Dicks
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Correspondence to Leon M. T. Dicks.

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Brand, A.M., Smith, R., de Kwaadsteniet, M. et al. Development of a Murine Model with Optimal Routes for Bacterial Infection and Treatment, as Determined with Bioluminescent Imaging in C57BL/6 Mice. Probiotics & Antimicro. Prot. 3, 125–131 (2011). https://doi.org/10.1007/s12602-011-9069-4

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