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Probiotic Lactobacillus and Bifidobacterial Lectins Against Candida albicans and Staphylococcus aureus Clinical Strains: New Class of the Pathogen Biofilm Destructors

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Abstract

Preparations of probiotic bifidobacterial and lactobacillus lectins possessed system affinity to mannan and mucin-type polymers. It was shown that these lectins possess fungistatic and fungicidal activities against nystatin-resistant Candida albicans clinical strains. Lectins revealed destructive properties with respect to C. albicans and Staphylococcus aureus biofilms, depending on clinical strain origin and lectin preparation type. Synergistic antipathogen activities between lectins and between lectins and nystatin were observed. In the presence of lectins, pathogen biofilm degradation occurred in sequential steps, including biofilm refinement, appearance of edge cavities, segmentation, detachment of fragments and their lysis. Fungal response to lectins was more complex compared to that of staphylococci. Cold stress improved pictures of lectin antipathogen action. The data indicate that probiotic bacterial lectins are members of a new class of antimicrobials—destructors of pathogen biofilms.

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Abbreviations

aLL:

Acidic lectins of lactobacilli

aLB:

Acidic lectins of bifidobacteria

bLL:

Basic lectins of lactobacilli

bLB:

Basic lectins of bifidobacteria

RPMI-HEPES:

RPMI-1640 plus HEPES pH 7.2–7.4

PAA(b):

Polyacrylamide (biotinylated)

PBS:

Phosphate saline buffer pH 7.2–7.4

PHA:

Phytohaemagglutinin

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Acknowledgments

The authors thank Prof. Chikindas and all other reviewers for fruitful discussion and value comments and recommendations. The authors also thank Katia Noll Sutyak for the technical help in preparing this article.

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Authors and Affiliations

  1. G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia

    M. Lakhtin, V. Alyoshkin, V. Lakhtin, S. Afanasyev, L. Pozhalostina & V. Pospelova

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  1. M. Lakhtin
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  2. V. Alyoshkin
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  3. V. Lakhtin
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  4. S. Afanasyev
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  5. L. Pozhalostina
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  6. V. Pospelova
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Correspondence to V. Lakhtin.

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Lakhtin, M., Alyoshkin, V., Lakhtin, V. et al. Probiotic Lactobacillus and Bifidobacterial Lectins Against Candida albicans and Staphylococcus aureus Clinical Strains: New Class of the Pathogen Biofilm Destructors. Probiotics & Antimicro. Prot. 2, 186–196 (2010). https://doi.org/10.1007/s12602-010-9046-3

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