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Antiviral Action of Methylated β-Lactoglobulin on the Human Influenza Virus A Subtype H3N2

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Abstract

Antiviral activity of methylated β-lactoglobulin (Met-BLG) against H3N2 infected into MDCK cell lines depended on concentration of Met-BLG, viral load, and duration of infection. IC50% of the hemagglutination activity for 1 and 0.2 MOI (multiplicity of infection) after 24 h of incubation at 37 °C in the presence of 5% CO2 were 20 ± 0.8 and 17 ± 0.7 μg mL−1 Met-BLG, respectively. Longer incubation period (4 days) was associated with low IC50% of the hemagglutination activity (7.1 ± 0.3 μg mL−1 Met-BLG) and low IC50% of immuno-fluorescence of viral nucleoproteins (9.7 ± 0.4 μg mL−1 Met-BLG) when using 0.2 and 0.1 MOI, respectively. A concentration of 25 μg mL−1 of Met-BLG reduced the amount of replicating virus by about 2 and 1.3 logs when the viral load was 0.01 and 0.1 MOI, respectively, while higher concentrations reduced it by about 5–6 logs. Antiviral action of Met-BLG was coupled with a cellular protective action, which reached 100% when using 0.01 and 0.1 MOI and 83% when using 1.0 MOI. The time of Met-BLG addition after the viral infection was determinant for its antiviral efficacy and for its protection of the infected MDCK cell lines. Anti-hemagglutination action and cell protective action decreased gradually and in parallel with the delay in the time of Met-BLG addition to disappear totally after 10 h delay.

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Acknowledgments

MS would like to thank for funding from ‘Imhotep’ Programme Hubert Curien and from Mission des Relations Internationales of INRA.

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Authors and Affiliations

  1. UR 1268 INRA, Biopolymères Interactions Assemblages, Equipe Fonctions et Interactions des Protéines Laitières, B.P. 71627, 44316, Nantes, Cedex 3, France

    Mahmoud Sitohy, Thomas Haertlé & Jean-Marc Chobert

  2. UFR des Sciences Pharmaceutiques, Bactériologie-Virologie, Université de Nantes, 1, rue Gaston Veil, B.P. 53508, 44035, Nantes, Cedex 1, France

    Bernard Besse & Sylviane Billaudel

  3. Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt

    Mahmoud Sitohy

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  1. Mahmoud Sitohy
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  2. Bernard Besse
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  3. Sylviane Billaudel
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  4. Thomas Haertlé
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  5. Jean-Marc Chobert
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Correspondence to Jean-Marc Chobert.

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Sitohy, M., Besse, B., Billaudel, S. et al. Antiviral Action of Methylated β-Lactoglobulin on the Human Influenza Virus A Subtype H3N2. Probiotics & Antimicro. Prot. 2, 104–111 (2010). https://doi.org/10.1007/s12602-010-9036-5

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