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Suppression of Listeria monocytogenes Scott A in Fluid Milk by Free and Liposome-Entrapped Nisin

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Abstract

Nisin is an antimicrobial polypeptide inhibitory toward Gram-positive bacterial pathogens, including Listeria monocytogenes. Encapsulating nisin in lipid nanocapsules (i.e., liposomes) has been shown to protect antimicrobial functionality in complex food matrices. The capacity of liposomes to encapsulate a fluorescent reporter was determined via spectroscopy. Survival and growth of L. monocytogenes incubated in fluid milk containing 50 IU/ml free or liposome-entrapped nisin was assayed via periodic enumeration of survivors. Liposomes were formulated from phosphatidylcholine (PC) and phosphatidyl-DL-glycerol (PG) and prepared as PC, PC/PG 7/3 or PC/PG 6/4 (mol. fraction). Antilisterial activity of nisin-loaded liposomes was determined in ultra-high temperature processed fluid milk containing approximately 4.0 log10 CFU/ml L. monocytogenes Scott A plus liposomal or free nisin at 50 IU/mL. Samples were aerobically held at 5 or 20°C; L. monocytogenes were enumerated via plating after 0, 1, 3, 6, 12, 24, 48, and 72 incubation hours. Liposome entrapment did not enhance pathogen inhibition when compared to free nisin as a function of storage temperature or incubation duration.

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Acknowledgments

This research was financially sponsored by Texas AgriLife Research (United States Department of Agriculture Hatch No. 09235). Authors wish to acknowledge Rhonda K. Miller, Ph.D., Professor of Meat Science, Department of Animal Science, Texas A&M University, for assistance provided with statistical analysis and interpretation of data.

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Authors and Affiliations

  1. Department of Animal Science, Texas Agri-Life Research, Texas A&M University, College Station, TX, 77843-2471, USA

    Shannon E. Schmidt, Glenn Holub & T. Matthew Taylor

  2. Department of Nutrition and Food Science, Texas Agri-Life Research, Texas A&M University, College Station, TX, 77843-2253, USA

    Joseph M. Sturino

Authors
  1. Shannon E. Schmidt
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  2. Glenn Holub
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  3. Joseph M. Sturino
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  4. T. Matthew Taylor
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Correspondence to T. Matthew Taylor.

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Schmidt, S.E., Holub, G., Sturino, J.M. et al. Suppression of Listeria monocytogenes Scott A in Fluid Milk by Free and Liposome-Entrapped Nisin. Probiotics & Antimicro. Prot. 1, 152–158 (2009). https://doi.org/10.1007/s12602-009-9022-y

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  • DOI: https://doi.org/10.1007/s12602-009-9022-y

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