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Detection and molecular characterization of sugarcane grassy shoot phytoplasma in Vietnam

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Abstract

Sugarcane is an important cash crop in Vietnam and has been widely promoted at national and provincial level. In 2006, a new disease was discovered in sugarcane in the Nghean Tate&Lyle Sugar Mill in Nghean Province in north-central Vietnam. The key symptoms were the formation of green grassy shoots around the base of mature stools. We applied nested polymerase chain reaction (PCR) using P1/P7 and R16F2n/R16R2 for detection and characterization of phytoplasma from the symptomatic tissues. PCR products of the expected size (approx. 1200 bp) were obtained from the 16S rDNA of the phytoplasma. The restriction fragment length polymorphism profiles indicated that all samples were infected by the same phytoplasma. Phylogenetic analysis confirmed that the SCGS phytoplasma from Vietnam belong to the 16SrXI group, formerly Rice Yellow Dwarf group.

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Acknowledgments

This work of T. X. Hoat was supported by the National Foundation for Science & Technology Development (NAFOSTED) (No. 106.15-2010.07).

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Authors and Affiliations

  1. Plant Protection Research Institute, Dongngac, Tuliem, Hanoi, Vietnam

    Trinh Xuan Hoat, Ngo Gia Bon, Mai Van Quan, Vu Duy Hien & Nguyen Duc Thanh

  2. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK

    Matthew Dickinson

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  1. Trinh Xuan Hoat
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  2. Ngo Gia Bon
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  3. Mai Van Quan
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  4. Vu Duy Hien
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Correspondence to Trinh Xuan Hoat.

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Hoat, T.X., Bon, N.G., Van Quan, M. et al. Detection and molecular characterization of sugarcane grassy shoot phytoplasma in Vietnam. Phytoparasitica 40, 351–359 (2012). https://doi.org/10.1007/s12600-012-0235-3

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