Skip to main content

Advertisement

Log in

Efficacy of biocontrol agents and natural compounds against powdery mildew of zucchini

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

The activity of different types of natural compounds and of two biofungicides based on Bacillus subtilis and Ampelomyces quisqualis alone and in combination with fungicides was tested against powdery mildew of zucchini. The efficacy was compared to the activity of fungicides used alone in four experimental trials carried out in the open field and under greenhouse conditions. The Podosphaera xanthii population used throughout the work was partially resistant to azoxystrobin, whereas it was susceptible to mychlobutanil. Sulphur plus terpenes and mustard oil consistently controlled powdery mildew, followed by mychlobutanil alone or in combination with A. quisqualis. B. subtilis and A. quisqualis when tested alone were partially effective. The combination of azoxystrobin and B. subtilis only delayed the spread of the pathogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Braun, U., & Takamatsu, S. (2000). Phylogeny of Erysiphe, Microsphaera, Uncinula (Erysipheae) and Cystotheca, Podosphaera, Sphaerotheca (Cystotheceae) inferred from rDNA ITS sequences – some taxonomic consequences. Schlechtendalia, 4, 1–33.

    Google Scholar 

  • Copping, L. G. (Ed.). (2004). The manual of biocontrol agents. Alton, Hampshire, UK: British Crop Protection Council.

    Google Scholar 

  • EPPO. (2004). EPPO Standards PP1 (2nd ed., Vol. 2). Paris, France: European and Mediterranean Plant Protection Organization.

    Google Scholar 

  • Gilardi, G., Manker, D. C., Garibaldi, A., & Gullino, M. L. (2008). Efficacy of the biocontrol agents Bacillus subtilis and Ampelomyces quisqualis applied in combination with fungicides against powdery mildew of zucchini. Journal of Plant Diseases and Protection, 115, 208–213.

    Google Scholar 

  • Hagiladi, A., & Ziv, O. (1986). The use of antitranspirants for the control of powdery mildew of roses in the field. Journal of Environmental Horticulture, 4, 69–71.

    Google Scholar 

  • Heaney, S. P., Hall, A. A., Davies, S. A., & Olaya, G. (2000). Resistance to fungicides in the QoI-STAR cross-resistance group: current perspectives. Proc. British Crop Protection Conference on Pests and Diseases, 2, 755–762.

    Google Scholar 

  • Hofstein, R., Daoust, R. A., & Aeschlimann, J. P. (1996). Constraints to the development of biofungicides: the example of AQ 10, a new product for controlling powdery mildews. Entomophaga, 41, 455–460.

    Article  Google Scholar 

  • Horst, R. K., Kawamoto, S. O., & Porter, L. L. (1992). Effect of sodium bicarbonate and oils on the control of powdery mildew and black spot of roses. Plant Disease, 76, 247–251.

    Article  CAS  Google Scholar 

  • Huggenberger, F., Collins, M. A., & Skylakakis, G. (1984). Decreased sensitivity of Sphaerotheca fuliginea to fenarimol and other ergosterol-biosynthesis inhibitors. Crop Protection, 3, 137–149.

    Article  Google Scholar 

  • Ishii, H. (2010). QoI fungicide resistance: current status and the problems associated with DNA-based monitoring. In U. Gisi, I. Chet, & M. L. Gullino (Eds.), Recent developments in management of plant diseases (pp. 37–45). Dordrecht, the Netherlands: Springer.

    Chapter  Google Scholar 

  • Jacobsen, B. J., Zidack, N. K., & Larson, B. J. (2004). The role of Bacillus-based biological control agents in integrated pest management systems: plant diseases. Phytopathology, 94, 1272–1275.

    Article  PubMed  CAS  Google Scholar 

  • Keinath, A. P., & DuBose, V. B. (2004). Evaluation of fungicides for prevention and management of powdery mildew on watermelon. Crop Protection, 23, 35–42.

    Article  CAS  Google Scholar 

  • Manker, D. C. (2005). Natural products as green pesticides. In J. M. Clark & H. Ohkawa (Eds.), New discoveries in agrochemicals (pp. 283–294). Washington, DC: American Chemical Society.

    Google Scholar 

  • Marrone, P. G. (2002). An effective biofungicide with novel modes of action. Pesticide Outlook, 13, 193–194.

    Article  CAS  Google Scholar 

  • Martin, B., Hernandez, S., Silvarrey, C., Jacas, J. A., & Cabaleion, C. (2005). Vegetable, fish and mineral oils control grapevine powdery mildew. Phytopathologia Mediterranea, 44, 169–179.

    Google Scholar 

  • Matheron, M. E., & Porchas, M. (2000). Evaluation of fungicide performance for control of powdery mildew on lettuce in 2000. Online publication no. AZ1177 in: Vegetable: College of Agriculture Report 2000. College of Agriculture, University of Arizona, Tucson, AZ, USA.

  • McGrath, M. T. (2001). Fungicide resistance in cucurbit powdery mildew, experiences and challenges. Plant Disease, 85, 236–245.

    Article  Google Scholar 

  • McGrath, M. T. (2007). Managing cucurbit powdery mildew and fungicide resistance. Acta Horticulturae, 731, 211–216.

    Google Scholar 

  • McGrath, M. T., & Shishkoff, N. (1999). Evaluation of biocompatible fungicides for managing cucurbit powdery mildew. Crop Protection, 18, 471–478.

    Article  Google Scholar 

  • Pasini, C., D’Aquila, F., Curir, P., & Gullino, M. L. (1997). Effectiveness of antifungal compounds against rose powdery mildew (Sphaerotheca pannosa var. rosae) in glasshouses. Crop Protection, 16, 251–256.

    Article  CAS  Google Scholar 

  • Paulitz, T. C., & Bélanger, R. B. (2001). Biological control in greenhouse systems. Annual Review of Phytopathology, 39, 103–133.

    Article  PubMed  CAS  Google Scholar 

  • Reuveni, R., & Reuveni, M. (1998). Foliar-fertilizer therapy – a concept in integrated pest management. Crop Protection, 17, 111–118.

    Article  CAS  Google Scholar 

  • Romero, D., Devicente, A., Rakotoaly, R. H., Dufour, S. E., Veening, J. W., Arrebola, E., et al. (2007). The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular Plant-Microbe Interactions, 20, 430–440.

    Article  PubMed  CAS  Google Scholar 

  • Shishkoff, N., & McGrath, M. T. (2002). AQ10 biofungicide combined with chemical fungicides or AddQ spray adjuvant for control of cucurbit powdery mildew in detached leaf culture. Plant Disease, 86, 915–918.

    Article  CAS  Google Scholar 

  • Sitterly, W. R. (1978). Powdery mildew of cucurbits. In D. M. Spencer (Ed.), The powdery mildews (pp. 359–379). London, UK: Academic Press.

    Google Scholar 

  • Winer, B. J. (1962). Statistical principles in experimental design (2nd ed.). New York, NY: McGraw-Hill.

    Book  Google Scholar 

  • Zitter, T. A., Hopkins, D. L., & Thomas, C. E. (1996). Compendium of cucurbit diseases. St. Paul, MN, USA: APS Press.

    Google Scholar 

Download references

Acknowledgments

This work was carried out with a grant from “Valorizzazione dell’orticoltura transfrontaliera VALORT” supported by the ALCOTRA programme of the European Union.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maria Lodovica Gullino.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gilardi, G., Baudino, M., Garibaldi, A. et al. Efficacy of biocontrol agents and natural compounds against powdery mildew of zucchini. Phytoparasitica 40, 147–155 (2012). https://doi.org/10.1007/s12600-011-0206-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-011-0206-0

Keywords

Navigation