Abstract
During a survey for mosaic disease caused by Cardamom mosaic virus (CdMV) in cardamom plantations, a new kind of viral disease showing chlorotic streak on veins was observed in some plantations. Based on the type of symptom, the disease was named ‘chlorotic streak’. In a survey of 77 cardamom plantations at 49 locations in the states of Kerala, Karnataka and Tamil Nadu in India, incidence of the disease ranged from zero to 15%. The plants infected with chlorotic streak tested negative in reverse transcription polymerase chain reaction (RT-PCR) for CdMV. Leaf dip electron microscopy of these samples showed the presence of flexuous virions resembling Potyvirus. Total RNA extracted from these plants when subjected to RT-PCR using primers targeted to conserved region in Potyvirus resulted in an amplicon size of ~700 bp. Sequencing and BLAST analysis of this amplicon showed Banana bract mosaic virus (BBrMV) as the closest virus. Using primers specific for BBrMV, the coat protein gene from five different geographical isolates of the virus was cloned and sequenced. Sequence analysis of the coat protein gene showed an identity of >94% with BBrMV isolates while identity with other distinct potyvirus species was <60%, indicating that the causal virus is a strain of BBrMV. Multiple sequence alignment and phylogenetic analyses showed high sequence conservation and close clustering of cardamom isolates along with a few banana isolates from India. This is the first report of the occurrence of BBrMV in cardamom. A reliable RT-PCR-based method was also developed for detection of the virus in cardamom plants.
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References
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.
Bateson, M. F., & Dale, J. L. (1995). Banana bract mosaic virus: characterization using potyvirus specific degenerate PCR primers. Archives of Virology, 140, 515–527.
Benson, D. A., Boguski, M. S. D., Lipman, J., Ostell, J., Ouellette, B. F., Rapp, B. A., et al. (1999). GenBank. Nucleic Acids Research, 27, 12–17.
Diekmann, M., & Putter, C. A. J. (1996). FAO/IPGRI technical guidelines for the safe movement of germplasm. No.15, Musa. 2nd ed. Food and Agriculture Organization of the United Nations. Rome, Italy: International Plant Genetic Resources Institute.
Dimitman, J. E., Floras, A., & Nikoloff, J. A. (1984). Cardamom mosaic - a member of the potyvirus group in Guatemala. Phytopathology, 74, 844.
Espino, T. M., Exconde, S. B., Zipagan, F. B., & Espino, R. R. C. (1990). Banana bract mosaic virus, a new disease of banana. II: Isolation and purification for monoclonal antibody production. Philippines Agriculture, 73, 61–68.
Gonsalves, D., Trujillo, E., & Hoch, H. C. (1986). Purification and some properties of a virus associated with cardamom mosaic, a new member of the potyvirus group. Plant Disease, 70, 65–69.
Govindaraju, C., Venugopal, M. N., & Sudharsan, M. R. (1994). An appraisal of 'kokke kandu' - a new viral disease of cardamom and katte (mosaic) disease in Karnataka. Journal of Plantation Crops, 22, 57–59.
Magnaye, L. V., & Espino, R. R. C. (1990). Banana bract mosaic, a new disease of banana. I. Symptomatology. Philippines Agriculture, 73, 55–59.
Mayne, W. W. (1951). Report on cardamom cultivation in South India. ICAR Bull. 50. New Delhi, India: Indian Council of Agricultural Research.
Pappu, S. S., Brand, R., Pappu, H. R., Rybicki, E., Gough, K. H., Frenkel, M. J., et al. (1993). A polymerase chain reaction method adapted for selective cloning of 3’ non translated regions of potyviruses: application to Dasheen mosaic virus. Journal of Virological Methods, 41, 9–20.
Ravindran, P. N. (2002). Introduction. In P. N. Ravindran & K. J. Madhusoodanan (Eds.), Cardamom. The genus Elettaria (pp. 1–10). London, UK/New York, NY: Taylor and Francis.
Rhoads, D. D., & Roufa, D. S. (1985). Emetine resistance of Chinese hamster cells: structure of wild type and mutant ribosomal protein S14 messenger RNA species. Molecular Cell Biology, 5, 1655–1659.
Rodoni, B. C., Ahlawat, Y. S., Varma, A., Dale, J. L., & Harding, R. M. (1999). The identification and characterization of banana bract mosaic in India. Plant Disease, 81, 669–672.
Siju, S., Madhubala, R., & Bhat, A. I. (2007). Sodium sulphite enhances RNA isolation and sensitivity of Cucumber mosaic virus detection by RT-PCR in black pepper. Journal of Virological Methods, 141, 107–110.
Thomas, J. E., Geering, A. D. W., Gambley, C. F., Kessling, A. F., & White, M. (1997). Purification, properties and diagnosis of banana bract mosaic potyvirus and its distinction from abaca mosaic potyvirus. Phytopathology, 87, 698–705.
Venugopal, M. N. (2002). Viral diseases of cardamom. In P. N. Ravindran & K. J. Madhusoodanan (Eds.), Cardamom. The genus Elettaria (pp. 143–159). London, UK/New York, NY: Taylor and Francis.
Acknowledgments
The financial assistance of Department of Biotechnology, Government of India, is gratefully acknowledged. We thank the In-charge, Unit of Plant Virology, Indian Agricultural Research Institute, New Delhi, for the electron microscope work.
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Siljo, A., Bhat, A.I., Biju, C.N. et al. Occurrence of Banana bract mosaic virus on cardamom. Phytoparasitica 40, 77–85 (2012). https://doi.org/10.1007/s12600-011-0193-1
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DOI: https://doi.org/10.1007/s12600-011-0193-1