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Selective chemotherapy on Grapevine leafroll-associated virus-1 and -3

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Abstract

Different chemotherapeutic strategies on closely correlated phytoviruses, such as Grapevine leafroll-associated virus-1 (GLRaV-1) and -3 (GLRaV-3), were tested in the same host in order to investigate selective chemotherapy of virus infections in plants. To eradicate these viruses from grapevine in vitro explants, antiviral treatments using heat or chemical drugs such as inosine monophosphate dehydrogenase (IMPDH) inhibitors (ribavirin, tiazofurin), purine biosynthesis inhibitors (6-thioguanine) and neuraminidase inhibitors (oseltamivir) were conducted. Phytotoxicity assay and thermal stress tests were performed before treatments; viruses were detected using ELISA and reverse transcriptase–polymerase chain reaction (RT-PCR). All chemicals were able to produce virus-free explants, but showed significantly different sanitation rates considering virus type. For GLRaV-1, higher sanitation rates were obtained using IMPDH inhibitors: 72.0% eradication was obtained upon administration of an exclusive inhibitor such as tiazofurin and 40.0% when using ribavirin. The most effective drugs against GLRaV-3 did not belong to IMPDH inhibitors: 78.0% virus-free explants were obtained using a neuraminidase inhibitor and 75.1% explants were sanitized using a purine biosynthesis inhibitor. Conversely, heat treatments showed similar efficacy against both viruses, achieving more than 55.0% virus-free explants, with a non-selective effect between GLRaV-1 and GLRaV-3. These findings suggest how highly correlated viruses could be differentiated in virus-specific events that are linked to selective chemotherapy of virus infections. However, virus-specific events that can influence chemical treatment generating selectivity did not seem to interfere with physical treatment.

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References

  • Barba, M., Cupidi, A., & Martino, L. (1990). Comparison of different methods to obtain virus-free grape propagative material. Proceedings of the X International Council for the Study of Viruses and Virus Diseases of the Grapevine (Volos, Greece, pp. 399–406).

  • Bertoni, P., Biricolti, S., Panattoni, A., & Triolo, E. (2000). Micropropagazione ed effetti di alcune molecole ad attività antivirale: indagini su ‘Sangiovese’. Proceedings of the International Symposium ‘Il Sangiovese’ (Firenze, Italy, p. 213).

  • Buciumeanu, E., & Visoiu, E. (2000). Elimination of grapevine viruses in V. vinifera L. cultivars. Proceedings of the 13th Meeting of the International Council for the Study of Viruses and Virus-like Diseases of the Grapevine (Adelaide, Australia, pp. 165–166).

  • Clark, M. F., & Adams, A. N. (1977). Characteristic of a microplate method of enzyme-linked immunoabsorbent assay for the detection of plant viruses. Journal of General Virology, 34, 375–483.

    Article  Google Scholar 

  • D’Anna, F. (2006). Antiviral thermo- and chemotherapy in vitro. Rome, Italy: Aracne Ed.

    Google Scholar 

  • De Clercq, E. (2001). Molecular targets for antiviral agents. The Journal of Pharmacology and Experimental Therapeutics, 297, 1–10.

    PubMed  Google Scholar 

  • Dolja, V. V., Kreuze, J. F., & Valkonen, J. P. T. (2006). Comparative and functional genomics of closteroviruses. Virus Research, 117, 38–51.

    Article  PubMed  CAS  Google Scholar 

  • Franchetti, P., Cappellacci, L., & Grifantini, M. (1996). IMP dehydrogenase as a target of antitumor and antiviral chemotherapy. II Farmaco, 51, 457–469.

    CAS  Google Scholar 

  • Gearry, R. B., & Barclay, M. (2005). Azothiopurine and 6-mercaptopurine pharmacogenetics and metabolite monitoring in inflammatory bowel disease. Journal of Gastroenterology and Hepatology, 20, 1149–1157.

    Article  PubMed  CAS  Google Scholar 

  • Grammatikaki, G., & Avgelis, A. (2009). Virus elimination in infected clones of four Greek grapevine wine cultivars (Roditis, Xinomavro, Baftra and Savatiano) using in vitro thermotherapy and tip meristem culture. Extended abstracts of the XVI Meeting of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (Dijon, France, pp. 241–242).

  • Gubareva, L. V. (2004). Molecular mechanisms of influenza virus resistance to neuraminidase inhibitors. Virus Research, 103, 199–203.

    Article  PubMed  CAS  Google Scholar 

  • Guta, I. C., Buciumeanu, E. C., Gheorghe, R. N., & Teodorescu, A. (2010). Solutions to eliminate grapevine leafroll associated virus serotype 1+3 from V. vinifera L. cv. Ranai Magaraci. Romanian Biotechnological Letters, 15, 72–78.

    Google Scholar 

  • Lozoya-Saldana, H., Dawson, W. O., & Murashige, T. (1984). Effects of ribavirin and adenine arabinoside on tobacco mosaic virus in Nicotiana tabacum L. var. Xanthi tissue cultures. Plant Cell, Tissue and Organ Culture, 3, 41–48.

    Article  CAS  Google Scholar 

  • Luvisi, A., Panattoni, A., & Triolo, E. (2011). Thiopurine prodrugs for plant chemotherapy purposes. Journal of Phytopathology, 159, 390–392.

    Article  CAS  Google Scholar 

  • MacKenzie, D. J., McLean, M. A., Mukerji, S., & Green, M. (1997). Improved RNA extraction from woody plants for the detection of viral pathogens by Reverse Transcription-Polymerase Chain Reaction. Plant Disease, 81, 222–226.

    Article  CAS  Google Scholar 

  • Maliogka, V. I., Dovas, C. I., & Katis, N. I. (2008). Evolutionary relationships of virus species belonging to a distinct lineage within the Ampelovirus genus. Virus Research, 135, 125–135.

    Article  PubMed  CAS  Google Scholar 

  • Monette, P. L. (1983). Virus eradication through in vitro techniques. The International Plant Propagators Society, 33, 90–100.

    Google Scholar 

  • Nakaune, R., & Nakano, M. (2006). Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. Journal of Virological Methods, 134, 244–249.

    Article  PubMed  CAS  Google Scholar 

  • Panattoni, A., D’Anna, F., & Triolo, E. (2006). Improvement in grapevine chemotherapy. Proceedings of the 15th Meeting of the International Council for the Study of Viruses and Virus-like Diseases of the Grapevine (Stellenbosch, South Africa, pp. 139–140).

  • Panattoni, A., D’Anna, F., & Triolo, E. (2007). Antiviral activity of tiazofurin and mycophenolic acid against Grapevine Leafroll-associated Virus 3 in Vitis vinifera explants. Antiviral Research, 73, 206–211.

    Article  PubMed  CAS  Google Scholar 

  • Schulze, S., & Kluge, S. (1994). The mode of inhibition of TMV- and PVX-induced RNA-dependent RNA polymerase by some antiphytoviral drugs. Journal of Phytopathology, 141, 77–85.

    Article  CAS  Google Scholar 

  • Skiada, F. G., Grigoriadou, K., Maliogka, V. I., Katis, N. I., & Eleftheriou, E. P. (2009). Elimination of Grapevine leafroll-associated virus 1 and Grapevine rupestris stem pitting-associated virus from grapevine cv. Agiorgitiko, and a micropropagation protocol for mass production of virus-free plantlets. Journal of Plant Pathology, 91, 177–184.

    CAS  Google Scholar 

  • Spiegel, S., Frison, E. A., & Converse, R. H. (1993). Recent developments in therapy and virus-detection procedures for international movement of clonal plant germ plasm. Plant Disease, 77, 1176–1180.

    Article  Google Scholar 

  • Valero, M., Ibanez, A., & Morte, A. (2003). Effects of high vineyard temperatures on the Grapevine leafroll associated virus elimination from Vitis vinifera L. cv. Napoleon tissue culture. Scientia Horticulturae, 97, 289–296.

    Article  Google Scholar 

  • Verma, N., Ram, R., & Zaidi, A. A. (2005). In vitro production of Prunus necrotic ringspot virus-free begonias through chemo- and thermotherapy. Scientia Horticulturae, 103, 239–247.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Tree Science, Entomology and Plant Pathology “G. Scaramuzzi”, University of Pisa, 56124, Pisa, Italy

    A. Panattoni, A. Luvisi & E. Triolo

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  1. A. Panattoni
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  2. A. Luvisi
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  3. E. Triolo
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Correspondence to A. Panattoni.

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Panattoni, A., Luvisi, A. & Triolo, E. Selective chemotherapy on Grapevine leafroll-associated virus-1 and -3 . Phytoparasitica 39, 503–508 (2011). https://doi.org/10.1007/s12600-011-0185-1

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  • DOI: https://doi.org/10.1007/s12600-011-0185-1

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