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Revealing molecular structure of starch with Stokes-vector based second harmonic generation microscopy

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Abstract

We report on the measurement and characterization of polarization properties of second harmonic (SH) light from starch granule using a Stokes vector based microscopy. The four-element Stokes parameters are the most complete description of the polarization state of SH light, including any depolarization effects. Various polarization parameters, such as the degree of polarization, the degree of linear polarization, the degree of circular polarization, as well as anisotropy are extracted by implementing a pixel by pixel image analysis from the 2D reconstructed SH Stokes images of starch granule. Furthermore, we implemented the technique by varying the polarization states of excitation light and recording the resulting Stokes parameters at micro-meter depths of resolution in order to investigate the molecular structure and orientation of the samples.

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Acknowledgements

The authors would like to thank Dr. Matthew R. Foreman, Dr. Carlos Macías Romero and Prof. Peter Török for helping us to understand the theory of Stokes vector formalism. The authors would also like to thank the National Science Council (Grant No. NSC99-2627-M-010-002), Taiwan for research funding.

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Authors and Affiliations

  1. Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, KA, India

    Nirmal Mazumder

  2. Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan

    Lu Yun-Xiang, Jianjun Qiu & Fu-Jen Kao

  3. Key Laboratory of Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China

    Jianjun Qiu

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  1. Nirmal Mazumder
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  2. Lu Yun-Xiang
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Correspondence to Nirmal Mazumder.

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Mazumder, N., Yun-Xiang, L., Qiu, J. et al. Revealing molecular structure of starch with Stokes-vector based second harmonic generation microscopy. J Opt 47, 40–46 (2018). https://doi.org/10.1007/s12596-017-0419-9

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