Abstract
Silver nanoparticles (Ag NPs) have been synthesized by chemical reduction in silver nitrate solution in the presence of the strong reducing agent sodium borohydride, NaBH4 and polyvinyl alcohol, and PVA was added as a stabilizer for Ag NPs by two methods: in situ and ex situ. Stability of Ag NPs has been studied by using DLS method. UV–Vis spectroscopy confirms the presence of silver nanoparticles in the solution in both the methods. PVA has been proved to stabilize Ag NPs comparatively better in the solution prepared by ex situ method than in situ method. Bare PVA film and both Ag/PVA nanocomposite films prepared by solution casting method were subjected to moisture absorption test, XRD, FESEM, EDX and antibacterial activity (against Escherichia coli) study. Ag NPs prepared by in situ process show better property with respect to moisture absorption and crystallinity. Again, the lower value of MIC (minimum inhibitory concentration) of the Ag/PVA NC film prepared via ex situ mode against E. coli shows better antibacterial property than Ag/PVA NC film prepared via in situ method suggests for food packaging application.
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Izatt RM, Rytting JH, Christensen JJ (1971) Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides. Chem Rev 71:439–481. https://doi.org/10.1021/cr60273a002
Garrard W, Lascelles J (1968) Regulation of Staphylococcus aureus lactate dehydrogenase. J Bacteriol 95:152–156
Guerlava P, Izac V, Tholozan JL (1998) Comparison of different methods of cell lysis and protein measurements in clostridium perfringens: application to the cell volume determination. Curr Microbiol 36:131–135. https://doi.org/10.1007/pl00006756
Fuhrmann GF, Rothstein A (1968) The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim Biophys Acta 163:331–338. https://doi.org/10.1128/aem.02001-07
Miller LP, McCallan SEA (1957) Toxic action of metal ions to fungus spores. J Agric Food Chem 5:116–122. https://doi.org/10.1021/jf60072a003
Prucek R, Kolar M, Kilianova M, Tucek J et al (2011) The targeted antibacterial and antifungal properties of magnetic nanocomposite of iron oxide and silver nanoparticles. Biomaterial 32:4704–4713. https://doi.org/10.1016/j.biomaterials.2011.03.039
Henglein A (1993) Physicochemical properties of small metal particles in solution: “microelectrode” reactions, chemisorption, composite metal particles, and the atom-to-metal transition. J Phys Chem 97:5457–5471. https://doi.org/10.1021/j100123a004
Huang ZY, Mills G, Hajek B (1993) Spontaneous formation of silver particles in basic 2-propanol. J Phys Chem 97:11542–11550. https://doi.org/10.1021/j100146a031
Pal T, Sau TK, Jana NR (2001) Seed-mediated successive growth of gold particles accomplished by UV irradiation: a photochemical approach for size-controlled synthesis. J Colloid Interface Sci 140:75–80. https://doi.org/10.1016/s1010-6030(01)00389-6
Henglein A (1989) Small-particle research: physicochemical properties of extremely small colloidal metal and semiconductor particles. Chem Rev 89:1861–1873. https://doi.org/10.1021/cr00098a010
El-Sayed MA (2001) Some interesting properties of metals confined in time and nanometer space of different shapes. Acc Chem Res 34:257. https://doi.org/10.1021/ar960016n
Pastoriza-Santos I, Liz-Marzan LM (2000) Binary cooperative complementary nanoscale interfacial materials. Reduction of silver nanoparticles in DMF. Formation of monolayers and stable colloids. Pure Appl Chem 72:83–90. https://doi.org/10.1351/pac200072010083
Lee JE, Kim JW, Jun JB et al (2004) Polymer/Ag composite microspheres produced by water-in-oil-in-water emulsion polymerization and their application for a preservative. Colloid Polym Sci 282:295–299. https://doi.org/10.1007/s00396-003-0943-9
Kim M, Byun JW, Shin DS, Lee YS (2009) Spontaneous formation of silver nanoparticles on polymeric supports. Mater Res Bull 44:334–338. https://doi.org/10.1016/j.materresbull.2008.05.014
Zhang S, Sun D, Fu Y, Du H (2003) Recent advances of superhard nanocomposite coatings: a review. Surf Coat Technol 167:113–119. https://doi.org/10.1016/S0257-8972(02)00903-9
Jung WK, Koo HC, Kim KW, Shin S, Kim SH, Park YH (2008) Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Am Soc Microbiol 74:2171–2178. https://doi.org/10.1128/aem.02001-07
Ramesh GV, Porel S, Radhakrishnan TP (2009) Polymer thin films embedded with in situ grown metal nanoparticles. Chem Soc Rev 38:2646–2656. https://doi.org/10.1039/b815242j
Porel S, Singh S, Harsha SS, Rao DN, Radhakrishnan TP (2005) Nanoparticle-embedded polymer: in situ synthesis, freestanding films with highly monodisperse silver nanoparticles and optical limiting. Chem Mater 17:9–12. https://doi.org/10.1039/b815242j
Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH et al (2007) Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol 3:95–101. https://doi.org/10.1016/j.nano.2006.12.001
Jain J, Arora S, Rajwade JM, Omray P, Khandelwal S, Paknikar KM (2009) Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Mol Pharm 6:1388–1401. https://doi.org/10.1021/mp900056g
Jones SA, Bowler PG, Walker M, Parsons D (2004) Controlling wound bioburden with a novel silver-containing Hydrofiber dressing. Wound Repair Regen 12:288–294. https://doi.org/10.1111/j.1067-1927.2004.012304.x
Pinto RJB, Marques PAAP, Neto CP, Trindade T, Daina S, Sadocco P (2009) Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomater 5:2279–2289. https://doi.org/10.1016/j.actbio.2009.02.003
Berger TJ, Spadaro JA, Chapin SE, Becker RO (1976) Electrically generated silver ions: quantitative effects on bacterial and mammalian cells. Antimicrob Agents Chemother 9:357–358
Rayman MK, Lo TC, Sanwal BD (1972) Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. J Biol Chem 247:6332–6339
Schreurs WJ, Rosenberg H (1982) Effect of silver ions on transport and retention of phosphate by Escherichia coli. J Bacteriol 152:7–13
Varshney R, Mishra AN, Bhadauria S, Gaur MS (2009) A novel microbial route to synthesis silver nanoparticles using fungus hormoconisresinae. Dig J Nanomater Biostruct 4:349–355
Retchkiman-Schabes PS, Canizal G, Becerra-Herrera R, Zorrilla C, Liu HB, Ascencio JA (2006) Biosynthesis and characterization of Ti/Ni bimetallic nanoparticles. Opt Mater 29:95–99. https://doi.org/10.1016/j.optmat.2006.03.014
Ahmad Z, Pandey R, Sharma S, Khuller GK (2006) Alginate nanoparticles as antituberculosis drug carriers: formulation development pharmacokinetics and therapeutic potential. Indian J Chest Dis Allied Sci 48:171–176
Won H, Nersisyan H, Won CW, Lee JM, Hwang JS (2010) Preparation of porous silver particles using ammonium formate and its formation mechanism. Chem Eng J 156:459–464. https://doi.org/10.1016/j.cej.2009.10.053
Tripathi S, Mehrotra GK, Dutta PK (2011) Chitosan–silver oxide nanocomposite film: preparation and antimicrobial activity. Bull Mater Sci 34:29–35. https://doi.org/10.1007/s12034-011-0032-5
Johnston JH, Borrmann T, Rankin D, Cairns M, Grindrod JE, McFarlane A (2008) Nano-structured composite calcium silicate and some novel applications. Curr Appl Phys 8:504–507. https://doi.org/10.1016/j.cap.2007.10.060
Tankhiwale R, Bajpai SK (2009) Graft copolymerization onto cellulose-based filter paper and its further development as silver nanoparticles loaded antibacterial food-packaging material. Colloids Surf B 69:164–168. https://doi.org/10.1016/j.colsurfb.2008.11.004
Hong KH, Park JL, Sul IH, Youk JH, Kang TJ (2006) Preparation of antimicrobial poly (vinyl alcohol) nanofibers containing silver nanoparticles. J Polym Sci, Part B: Polym Phys 44:2468–2474. https://doi.org/10.1002/polb.2091
An J, Zhang M, Wang S, Tang J (2008) Physical, chemical and microbiological changes in stored green asparagus spears as affected by coating of silver nanoparticles-PVP. LWT Food Sci Technol 41:1100–1107. https://doi.org/10.1016/j.lwt.2007.06.019
Li H, Li F, Wang L, Sheng J, Xin Z, Zhao L, Xiao H, ZhengY HuQ (2009) Effect of nano-packing on preservation quality of Chinese jujube (Ziziphus jujuba Mill. var. inermis (Bunge) Rehd). Food Chem 114:547–552. https://doi.org/10.1016/j.foodchem.2008.09.085
Kong H, Jang J (2008) Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles. Langmuir 24:2051–2056. https://doi.org/10.1021/la703085e
Sheikh FA, Barakat NAM, Kanjwal MA, Chaudhari AA, Jung IH, Lee JH, Kim HY (2009) Electrospun antimicrobial polyurethane nanofibers containing silver nanoparticles for biotechnological applications. Macromol Res 17:688–696. https://doi.org/10.1007/bf03218929
Fu J, Ji J, Fan D, Shen JJ (2006) Construction of antibacterial multilayer films containing nanosilver via layer-by-layer assembly of heparin and chitosan-silver ions complex. J Biomed Mater Res A 79:665–674. https://doi.org/10.1002/jbm.a.30819
Jung R, Kim Y, Kim HS, Jin HJ (2009) Antimicrobial properties of hydrated cellulose membranes with silver. J Biomater Sci 20:311–324. https://doi.org/10.1163/156856209x412182
Yoksan R, Chirachanchai S (2010) Silver nanoparticle-loaded chitosan–starch based films: fabrication and evaluation of tensile, barrier and antimicrobial properties. Mater Sci Eng, C 30:891–897. https://doi.org/10.1016/j.msec.2010.04.004
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The authors gratefully thank Dr. P. S. Ray of Indian Institute of Science, Education and Research, West Bengal, for carrying out the evaluation of antibacterial activity in their laboratory.
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Das, R.K., Das, M. Study of silver nanoparticle/polyvinyl alcohol nanocomposite. Int J Plast Technol 23, 101–109 (2019). https://doi.org/10.1007/s12588-019-09229-4
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DOI: https://doi.org/10.1007/s12588-019-09229-4