Skip to main content
Log in

Long-term rearing of Japanese eel larvae using a liquid-type diet: food intake, survival and growth

  • Original Article
  • Aquaculture
  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

The efficiency of a new liquid-type diet for long-term rearing of Japanese eel larvae until metamorphosis was examined, as was the effect of diet viscosity on diet intake and on the survival and growth of early larvae. The highest intake of the experimental diet by 6- and 9-day post-hatch (dph) larvae occurred at viscosities of 20–50 mPa·s, much lower than the viscosity of the currently used slurry-type diet (ca. 2900 mPa·s). Long-term feeding trials for 259 days (n = 4) showed that overall survival rates of larvae (37–59%) fed a liquid-type diet with lower viscosity (40–680 mPa·s) were 2 to 3.4 times higher than the survival rates of larvae fed the slurry-type diet (11–25%). Because nutrients were diluted in the liquid-type diet, the growth of larvae fed this diet was slower after about 200 days and metamorphosis was delayed. However, the yield of glass eels was 1.1 to 3.2 times higher in larvae fed the liquid than the slurry diet. These findings suggest that feeding the liquid-type diet can result in the mass production of glass eels by ensuring high growth, survival and metamorphosis rates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Bouilliart M, Tomkiewicz J, Lauesen P, De Kegel B, Adriaens D (2015) Musculoskeletal anatomy and feeding performance of pre-feeding engyodontic larvae of the European eel (Anguilla anguilla). J Anat 227:325–340. https://doi.org/10.1111/joa.12335

    Article  PubMed  PubMed Central  Google Scholar 

  • Feunteun E, Miller MJ, Carpentier A, Aoyama J, Dupuy C, Kuroki M, Pagano M, Réveillac E, Sellos D, Watanabe S, Tsukamoto K, Otake T (2015) Stable isotopic composition of anguilliform leptocephali and other food web components from west of the Mascarene Plateau. Prog Oceanogr 137:69–83. https://doi.org/10.1016/j.pocean.2015.05.024

    Article  Google Scholar 

  • Horie N, Utoh T, Mikawa N, Yamada Y, Okamura A, Tanaka S, Tsukamoto K (2008) Influence of artificial fertilization methods of the hormone-treated Japanese eel Anguilla japonica upon the quality of eggs and larvae (comparison between stripping-insemination and spontaneous spawning methods). Nippon Suisan Gakkaishi 74:26–35 (in Japanese with English abstract)

    Article  Google Scholar 

  • Kuroki M, Fukuda N, Yamada Y, Okamura A, Tsukamoto K (2010) Morphological changes and otolith growth during metamorphosis of Japanese eel leptocephali in captivity. Coast Mar Sci 34:31–38

    Google Scholar 

  • Masuda Y, Oku H, Nomura K, Teruya K, Tanaka H (2010) A colloid-type diet can be ingested by larvae of the Japanese eel Anguilla japonica. J Fish Technol 2:99–104 (in Japanese with English abstract)

    Google Scholar 

  • May RC (1974) Larval mortality in marine fishes and the critical period concept. In: Blaxter JHS (ed) The early life history of fish. Springer, Berlin, pp 3–19

    Chapter  Google Scholar 

  • Miller MJ, Chikaraishi Y, Ogawa NO, Yamada Y, Tsukamoto K, Ohkouchi N (2012) A low trophic position of Japanese eel larvae indicates feeding on marine snow. Biol Lett 9:20120826. https://doi.org/10.1098/rsbl.2012.0826

    Article  PubMed  Google Scholar 

  • Mochioka N, Iwamizu M (1996) Diet of anguilloid larvae: leptocephali feed selectively on larvacean houses and fecal pellets. Mar Biol 125:447–452. https://doi.org/10.1007/BF00353257

    Article  Google Scholar 

  • Ohta H, Kagawa H, Tanaka H, Okuzawa K, Iinuma N, Hirose K (1997) Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica. Fish Physiol Biochem 17:163–169

    Article  CAS  Google Scholar 

  • Okamura A, Yamada Y, Mikawa N, Horie N, Utoh T, Kaneko T, Tanaka S, Tsukamoto K (2009a) Growth and survival of eel leptocephali (Anguilla japonica) in low-salinity water. Aquaculture 296:367–372. https://doi.org/10.1016/j.aquaculture.2009.08.039

    Article  CAS  Google Scholar 

  • Okamura A, Yamada Y, Horita T, Horie N, Mikawa N, Utoh T, Tanaka S, Tsukamoto K (2009b) Rearing eel leptocephali (Anguilla japonica Temminck & Schlegel) in a planktonkreisel. Aquac Res 40:509–512. https://doi.org/10.1111/j.1365-2109.2008.02127.x

    Article  Google Scholar 

  • Okamura A, Yamada Y, Mikawa N, Horie N, Tsukamoto K (2012) Effect of starvation, body size, and temperature on the onset of metamorphosis in Japanese eel (Anguilla japonica). Can J Zool 90:1378–1385. https://doi.org/10.1139/cjz-2012-0146

    Article  Google Scholar 

  • Okamura A, Yamada Y, Horie N, Mikawa N, Tanaka S, Kobayashi H, Tsukamoto K (2013) Hen egg yolk and skinned krill as possible foods for rearing leptocephalus larvae of Anguilla japonica Temminck & Schlegel. Aquac Res 44:1531–1538. https://doi.org/10.1111/j.1365-2109.2012.03160.x

    Article  CAS  Google Scholar 

  • Okamura A, Horie N, Mikawa N, Yamada Y, Tsukamoto K (2014) Recent advances in artificial production of glass eels for conservation of anguillid eel populations. Ecol Freshw Fish 23:95–110. https://doi.org/10.1111/eff.12086

    Article  Google Scholar 

  • Otake T, Nogami K, Maruyama K (1993) Dissolved and particulate organic matter as possible food sources for eel leptocephali. Mar Ecol Prog Ser 92:27–34

    Article  Google Scholar 

  • Riemann L, Alfredsson H, Hansen MM, Als TD, Nielsen TG, Munk P, Aarestrup K, Maes GE, Sparholt H, Petersen MI, Bachler M, Castonguay M (2010) Qualitative assessment of the diet of European eel larvae in the Sargasso Sea resolved by DNA barcoding. Biol Lett 6:819–822. https://doi.org/10.1098/rsbl.2010.0411

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tanaka H (2015) Progression in artificial seedling production of Japanese eel Anguilla japonica. Fish Sci 81:11–19. https://doi.org/10.1007/s12562-014-0821-z

    Article  CAS  Google Scholar 

  • Tanaka H, Kagawa H, Ohta H (2001) Production of leptocephali of Japanese eel (Anguilla japonica) in captivity. Aquaculture 201:51–60. https://doi.org/10.1016/S0044-8486(01)00553-1

    Article  Google Scholar 

  • Yamada Y, Okamura A, Mikawa N, Utoh T, Horie N, Tanaka S, Miller M, Tsukamoto K (2009) Ontogenetic changes in phototactic behavior during metamorphosis of artificially reared Japanese eel Anguilla japonica larvae. Mar Ecol Prog Ser 379:241–251. https://doi.org/10.3354/meps07912

    Article  Google Scholar 

  • Yamada Y, Okamura A, Mikawa N, Horie N, Tsukamoto K (2019) A new liquid-type diet for leptocephali in mass production of artificial glass eels. Fish Sci 85:545–551. https://doi.org/10.1007/s12562-019-01295-2

    Article  CAS  Google Scholar 

  • Yoshimatsu T (2011) Early development of preleptocephalus larvae of the Japanese eel in captivity with special reference to the organs for larval feeding. Bull Grad Sch Bioresour Mie Univ 37:11–18

    Google Scholar 

Download references

Acknowledgements

This study was partially supported by the research fund from Toyo Suisan Kaisha. Ltd. and JSPS KAKENHI grant numbers JP26450268, 26252030, 17K19300 to KT.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Akihiro Okamura.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Okamura, A., Yamada, Y., Horie, N. et al. Long-term rearing of Japanese eel larvae using a liquid-type diet: food intake, survival and growth. Fish Sci 85, 687–694 (2019). https://doi.org/10.1007/s12562-019-01316-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12562-019-01316-0

Keywords

Navigation