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Determination of periods of sensitivity to low-salinity and low-temperature conditions during the early development of cultured Japanese eel Anguilla japonica larvae with respect to the rate of morphological deformity at completion of yolk resorption

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  • Aquaculture
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Abstract

The present study analyzed the rate of occurrence of deformities at completion of yolk resorption after Japanese eel larvae were exposed to low-salinity (29 psu) or low-water-temperature (20 °C) conditions (after they had been reared under suitable conditions: 34 psu, 25 °C) at various points between 0 and 8 days post-fertilization (dpf) in order to determine the period of sensitivity of the larvae to low-salinity or low-temperature conditions. No significant differences were observed between the rate of deformed larvae obtained in the groups reared under suitable conditions and the corresponding rates in groups that were exposed to low-salinity or low-temperature conditions at 4 dpf or later, but the frequency of normal larvae showed a downward trend in experimental groups that were subjected to low-salinity or low-temperature conditions at 3 dpf or earlier. Occurrence rates of pericardial edema were significantly higher in experimental groups that were exposed to low-salinity or low-temperature conditions at 2 dpf or earlier. Therefore, it was concluded that the rearing conditions for Japanese eel larvae should be maintained at 34 psu salinity and 25 °C until at least 4 dpf in order to reduce the occurrence of larval deformity at completion of yolk resorption.

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Acknowledgments

We would like to express our thanks to the staff of IRAGO Institute Co., Ltd., Aichi, Japan and the Freshwater Resources Research Center, Aichi Fisheries Research Institute for providing the eel broodstock used in the present study. The present study was supported by a grant-in-aid (Development of Sustainable Aquaculture Technology Independent of Wild Fishery Resources) from the Ministry of Agriculture, Forestry and Fisheries, Government of Japan.

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Author notes

  1. Koichiro Gen

    Present address: Seikai National Fisheries Research Institute, Fisheries Research Agency, 1551-8, Taira-machi, Nagasaki-shi, Nagasaki, 851-2213, Japan

Authors and Affiliations

  1. Tohoku National Fisheries Research Institute, Fisheries Research Agency, 3-27-5 Shinhama, Shiogama, Miyagi, 985-0001, Japan

    Tadahide Kurokawa

  2. National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minamiise, Mie, 516-0193, Japan

    Hideyuki Shibahara, Kazuharu Nomura & Hideki Tanaka

  3. Tokyo University of Marine Science and Technology, Konan Minato, Tokyo, 108-8477, Japan

    Hideyuki Shibahara

  4. Tamaki Station, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1, Hiruta, Tamaki, Mie, 519-0423, Japan

    Koichiro Gen

Authors
  1. Tadahide Kurokawa
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  2. Hideyuki Shibahara
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  3. Koichiro Gen
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  4. Kazuharu Nomura
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  5. Hideki Tanaka
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Correspondence to Tadahide Kurokawa.

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Kurokawa, T., Shibahara, H., Gen, K. et al. Determination of periods of sensitivity to low-salinity and low-temperature conditions during the early development of cultured Japanese eel Anguilla japonica larvae with respect to the rate of morphological deformity at completion of yolk resorption. Fish Sci 79, 673–680 (2013). https://doi.org/10.1007/s12562-013-0636-3

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  • DOI: https://doi.org/10.1007/s12562-013-0636-3

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