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Effects of n-3 highly unsaturated fatty acid content in Artemia on survival and development of laboratory-reared horsehair crab Erimacrus isenbeckii larvae

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  • Aquaculture
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Abstract

The effect of varying levels of dietary n-3 highly unsaturated fatty acid (HUFA) content in Artemia sp. on survival, the number of days required to reach each larval stage, and the growth and morphogenesis of larvae of the horsehair crab Erimacrus isenbeckii were examined. Artemia enriched with materials of six types [condensed Nannochloropsis sp., ethyl oleate, two enrichment levels of eicosapentaenoic acid (EPA), and those of docosahexaenoic acid], and unenriched Artemia were fed to larvae. Newly hatched larvae were reared in groups of 50 individuals in two 2-l beakers until they reached the first crab stage. No significant difference was found in survival rate, growth, or morphogenesis of larvae, but fewer days tended to be required to reach the first crab stage in the group enriched with high EPA compared to the other groups. Results show that, although the amount of n-3 HUFA in unenriched Artemia might meet the demands of horsehair crab larvae for their survival, growth, and morphogenesis, Artemia should be enriched with high EPA to improve the rate of larval development.

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Acknowledgments

We thank the staff of the Akkeshi Station of the Hokkaido National Fisheries Research Institute, Fisheries Research Agency, and the technology development team of seed production for crustaceans organized within Fisheries Research Agency for helpful assistance, as well as the anonymous reviewers for their helpful advice.

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Authors and Affiliations

  1. Shibushi Laboratory, National Research Institute of Aquaculture, Fisheries Research Agency, Natsui, Shibushi, Kagoshima, 899-7101, Japan

    Tadao Jinbo

  2. Tamano Laboratory, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Tamano, Okayama, 706-0002, Japan

    Shigeki Dan & Masakazu Ashidate

  3. Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Mitsuhiro Nakaya

  4. Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato, Tokyo, 108-8477, Japan

    Katsuyuki Hamasaki

Authors
  1. Tadao Jinbo
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  2. Shigeki Dan
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  3. Mitsuhiro Nakaya
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  4. Masakazu Ashidate
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  5. Katsuyuki Hamasaki
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Correspondence to Tadao Jinbo.

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Jinbo, T., Dan, S., Nakaya, M. et al. Effects of n-3 highly unsaturated fatty acid content in Artemia on survival and development of laboratory-reared horsehair crab Erimacrus isenbeckii larvae. Fish Sci 79, 459–467 (2013). https://doi.org/10.1007/s12562-013-0618-5

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  • DOI: https://doi.org/10.1007/s12562-013-0618-5

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