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Distribution of adipocyte-related cells in skeletal muscle of rainbow trout Oncorhynchus mykiss

  • Original Article
  • Chemistry and Biochemistry
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Abstract

Adiponectin is one of the adipokines secreted mainly from adipocytes in mammals. In rainbow trout, however, adiponectin is highly expressed in skeletal muscle. Although it has been suggested that fish skeletal muscle contains adipocytes, their endocrine function and distribution are poorly understood. Recently, an EST analysis of rainbow trout found that heart-type fatty acid binding protein (H-FABP), a member of the intracellular fatty acid binding protein family, encodes an adipose-specific gene. In this study, we produced anti-adiponectin and H-FABP antibodies and investigated the distribution of adipocytes and related cells in skeletal muscle of rainbow trout. The adiponectin signal was detected at around 75 kDa in muscle in Western blotting. Since the molecular mass of rainbow trout adiponectin is around 25 kDa, this 75 kDa band would be a trimer. For H-FABP, the signal band was detected at around 15 kDa. Immunohistochemistry of skeletal muscle sections indicated that adiponectin and H-FABP signals were present outside of muscle cells and throughout the muscle tissues, suggesting the existence of adipocyte-related cells in these regions. These results will contribute to our understanding of energy metabolism in fish skeletal muscle.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (A), no. 21248027, from the Ministry of Education, Science, Sports and Culture, and by the Program for Promotion of Basic Research Activities for Innovative Biosciences.

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Authors and Affiliations

  1. Laboratory of Marine Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan

    Yuna Han, Gen Kaneko, Shugo Watabe & Hideki Ushio

  2. Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan

    Hidehiro Kondo & Ikuo Hirono

  3. Laboratory of Food Chemistry and Functional Nutrition, Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan

    Reiko Nagasaka

  4. Laboratory of Molecular Food Function, Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan

    Hideki Ushio

  5. Laboratory of Animal Cell Regulation, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan

    Shin-Ichiro Takahashi

  6. Kitasato University School of Marine Biosciences, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa, 252-0373, Japan

    Shugo Watabe

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  1. Yuna Han
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  2. Gen Kaneko
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  3. Reiko Nagasaka
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  4. Hidehiro Kondo
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  6. Shin-Ichiro Takahashi
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  7. Shugo Watabe
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  8. Hideki Ushio
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Correspondence to Hideki Ushio.

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Han, Y., Kaneko, G., Nagasaka, R. et al. Distribution of adipocyte-related cells in skeletal muscle of rainbow trout Oncorhynchus mykiss . Fish Sci 79, 143–148 (2013). https://doi.org/10.1007/s12562-012-0579-0

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  • DOI: https://doi.org/10.1007/s12562-012-0579-0

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