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Antifouling steroids isolated from red alga epiphyte filamentous bacterium Leucothrix mucor

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  • Chemistry and Biochemistry
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Abstract

Active antifouling steroids were isolated from the filamentous bacterium Leucothrix mucor. Bioactivity-guided isolation by silica gel column chromatography and high-performance liquid chromatography generated two compounds. The chemical constituents having the antifouling activities were identified as 17-(1,2-dihydroxyl-5-methyl-hexane)-2,3-dihydroxyl-cholest-4-en-6-one [compound (a)] and 13-acetate-17-(1,5-dimethylhexane)-cholest-7-en-3,5,6,15-tetraol [compound (b)] by interpreting one- and two-dimensional nuclear magnetic resonance and mass spectroscopy data. The two compounds were isolated from 780 mg of crude extract, yielding 12 and 14 mg, respectively. Antifouling activities were determined against a representative soft fouling macroalgae (Ulva pertusa), a biofouling diatom (Navicula annexa), and the bacteria Pseudomonas aeruginosa KNP-3 and Alteromonas sp. KNS-8. The two compounds were able to inhibit the spore settlement of Ulva pertusa zoospores with a low half maximal effective concentration (EC50) (1.2–2.1 μg/ml) and inhibit diatom growth with an EC50 of 5.2–7.5 μg/ml. Compound (a) showed activity with minimum inhibitory concentration values of 32–56 μg/ml and compound (b) showed an activity of 66–90 μg/ml. Among the two compounds, compound (a) is a new compound, whereas compound (b) was previously reported to have been isolated from a marine invertebrate.

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Acknowledgments

This work was supported by the Soonchunhyang University Research Fund (No. 20080154).

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  1. Department of Marine Biotechnology, SoonChunHyang University, Asan, 336-745, Korea

    Ji Young Cho

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  1. Ji Young Cho
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Correspondence to Ji Young Cho.

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Cho, J.Y. Antifouling steroids isolated from red alga epiphyte filamentous bacterium Leucothrix mucor . Fish Sci 78, 683–689 (2012). https://doi.org/10.1007/s12562-012-0490-8

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  • DOI: https://doi.org/10.1007/s12562-012-0490-8

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