Abstract
It has been reported that the nucleotide sequences of the mitochondrial and nuclear genes of Takifugu pufferfish torafugu T. rubripes and karasu T. chinensis show 99–100% sequence identity, indicating a very close relationship between the two species. To further investigate this genetic relationship, we compared genetic variation at four microsatellite loci and at the mitochondrial control region (CR) (561 bp) between groups of T. rubripes caught at two locations [TrG, caught in the Genkai Sea off Tsushima Island in 2003 (n = 50); TrS, caught in the Suwo Sea off Kita-Kyushu in 2008 (n = 50)] and T. chinensis caught at one location (TcK, caught off the east coast of Korea in 2004; n = 50). Analyses using microsatellite loci showed that genetic diversity index values of the TrG, TrS and TcK groups were 0.9505, 0.9350 and 0.9335, respectively, while values of genetic distance and genetic differentiation between TrG and TcK (0.0543 and 0.0189, respectively) were smaller than those between TrG and TrS (0.0857 and 0.0194, respectively). Analyses using CR for the same specimens showed that genetic distances were consistent with those obtained using microsatellite loci. These results, together with our previous observations, suggest that T. rubripes and T. chinensis are very closely related and possibly can be regarded as the same species.
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Acknowledgments
We thank M. Nishida, Atmosphere and Ocean Research Institute, The University of Tokyo for his invaluable advice and for providing assistance in conducting the initial experiments for genotyping the microsatellite markers using fluorescently labeled primers with a GeneScan and Genotyper software (Applied Biosystems). We also express sincere thanks to H. Doi, Shimonoseki Marine Science Museum “Kaikyokan”, Shimonoseki Academy of Marine Science for helping with the collection of pufferfish samples from the Suwo Sea off Kitakyushu.
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Reza, M.S., Kinoshita, S., Furukawa, S. et al. Microsatellite and mitochondrial DNA analyses reveal no genetic difference between two pufferfish species torafugu Takifugu rubripes and karasu T. chinensis . Fish Sci 77, 59–67 (2011). https://doi.org/10.1007/s12562-010-0310-y
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DOI: https://doi.org/10.1007/s12562-010-0310-y