Abstract
Samples from large (100–200 tons) batches of palm kernel cake (PKC, n = 20) and copra meal (CM, n = 13) were collected at production facilities of four Indonesian feed mill manufacturers and analysed for aflatoxin B1 (AFB1) by ELISA. Recoveries using spiked samples ranged from 86 to 113 %, with relative standard deviations of <9 % (PKC) and <6 % (CM). All batches were positive for AFB1: in PKC, at levels of 5.8–93.1 μg/kg (mean 49 μg/kg), and in CM, at levels of 1.1–147 μg/kg (mean 38.1 μg/kg). AFB1 levels were, in most batches, below the maximum level (100 μg/kg) recommended by the National Standardisation Agency, Republic of Indonesia. However, about half of the batches exceeded both the European Union and USA regulations for AFB1 in animal feed. In conclusion, serious efforts are necessary to control production, storage and shipment of palm kernel cake and copra meal for feed purposes, and clearly not only for products intended for export but also to reduce AFB1 levels in domestic Indonesian feed.
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Aknowledgments
The authors acknowlege financial assistance by the European Union-funded Integrated Project Biotracer (contract 036272) under the 6th RTD Framework, and by the Indonesian Ministry of National Education for the support of the research under the Institution-Collaboration Research Grant (Contract No LPPM-UGM/669/bid.1/2012).
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Pranowo, D., Nuryono, Agus, A. et al. A limited survey of aflatoxin B1 contamination in Indonesian palm kernel cake and copra meal sampled from batches. Mycotoxin Res 29, 135–139 (2013). https://doi.org/10.1007/s12550-013-0168-9
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DOI: https://doi.org/10.1007/s12550-013-0168-9