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Fusarium fungi and associated metabolites presence on grapes from Slovakia

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Abstract

Toxinogenic Fusarium species were identified on grape berries from Slovak vineyards, and their toxic metabolites were analysed by HPLC-MS/MS. F. subglutinans, F. oxysporum, F. proliferatum, F. semitectum, F. solani, F. subglutinans, and F. verticillioides were found with varying frequency. F. oxysporum and F. proliferatum, cultured in vitro on Czapek yeast autolysate agar and yeast extract sucrose agar, produced beauvericin, in the range from 3,265 to 13,400 μg/kg, and fusaproliferin in high concentration, ranging from 49,850 to 259,500 μg/kg. A maximum value of 2.24 μg/kg has been observed for beauvericin in dried grape berries. Fumonisin B1, and fumonisin B2 were also identified, and the observed levels ranged from 500 to 2,040 μg/kg. Over 2 years (namely 2008 and 2009) many other metabolites have been identified and analysed in grape berries, in particular: avenacein Y, apicidin, aurofusarin, chlamydosporol, 2-amino-14,16-dimethyloctadecan-3-ol, enniatin A, enniatin A1, enniatin B2, enniatin B3, and equisetin.

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Acknowledgement

The work was supported by Project VEGA No. 2/0190/13 and MycoRed.

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Authors and Affiliations

  1. Department of Mycology and Physiology, Institute of Botany Slovak Academy of Sciences, Dubravská 9, 84523, Bratislava, Slovak Republic

    Petra Mikušová & Antónia Šrobárová

  2. Center for Analytical Chemistry, Department of Agrobiotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenzstr. 20, 3430, Tulln, Austria

    Michael Sulyok

  3. Department of Food Science, University of Napoli “Federico II”, Via Università 100, 80055, Portici (Napoli), Italy

    Antonello Santini

Authors
  1. Petra Mikušová
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  2. Antónia Šrobárová
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  3. Michael Sulyok
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  4. Antonello Santini
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Corresponding author

Correspondence to Antonello Santini.

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Mikušová, P., Šrobárová, A., Sulyok, M. et al. Fusarium fungi and associated metabolites presence on grapes from Slovakia. Mycotoxin Res 29, 97–102 (2013). https://doi.org/10.1007/s12550-013-0157-z

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  • DOI: https://doi.org/10.1007/s12550-013-0157-z

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