Skip to main content
SpringerLink
Log in

Assessment of mycoflora and infestation of insects, vector of Aspergillus section Flavi, in stored peanut from Argentina

  • Original Paper
  • Published:
Mycotoxin Research Aims and scope Submit manuscript

Abstract

The occurrence of spoilage fungi and Aspergillus section Flavi populations, the aflatoxins incidence, the role of insects as vectors of mycotoxin-producing fungi and the AFs-producing ability of the isolated species throughout the peanut (Arachis hypogaea L.) storage period were evaluated. Analyses of fungal populations from 95 peanut seed samples did not demonstrate significant differences between the incidences in each sampling period. Aspergillus section Flavi were isolated during all incubation periods. Cryptolestes spp. (Coleoptera: Cucujidae) were collected in August, September and October with 18, 16 and 28% of peanut samples contaminated, respectively. Insects isolated during August showed 69% of Aspergillus section Flavi contamination. A. flavus was the most frequently isolated (79%) from peanut seeds and from insect (59%). The greater levels of AFB1 were detected in September and October with a mean of 68.86 μg/kg and 69.12 μg/kg respectively. The highest proportion of A. flavus toxigenic strains (87.5%) was obtained in June. The presence of Aspergillus section Flavi and insect vectors of aflatoxigenic fungi presented a potential risk for aflatoxin production during the peanut storage period. Integrated management of fungi and insect vectors is in progress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adebesin AA, Saromi OT, Amusa NA, Fagade SO (2001) Microbiological quality of some groundnut products hawked in Bauchi, a Nigerian City. J Food Technol Af 6:53–55

    Google Scholar 

  • Ahmed EM, Pattee HE (1987) Peanut quality: Its assurance and maintenance from the farm to end-product, University of Florida, Institute of Food and Agricultural Science, pp 874

  • Barros G, Torres A, Chulze S (2005) Aspergillus flavus population isolated from soil of Argentina’s peanut-growing region. Sclerotia production and toxigenic profile. J Sci Food Agric 85:2349–2353

    Article  CAS  Google Scholar 

  • Bronswijk JEMH v, Sinha RN (1973) Role of fungi in the survival of Dermatophagoides (Acarina: Pyroglyphidae) in house-dust environment. Environ Entomol 2:142–145

    Google Scholar 

  • Carter JBH (1973) The influence of the testa, damage and seed dressing on the emergence of groundnut (Arachis hypogaea). Ann Appl Biol 74:315–323

    Article  Google Scholar 

  • Coulombe RA Jr (1993) Biological actions of mycotoxins. J Dairy Sci 76:880–891

    Article  CAS  PubMed  Google Scholar 

  • Dowd PF (1998) Involvement of arthropods in the establishment of mycotoxigenic fungi under field conditions. In: Sinha KK, Bhatnagar D (eds) Mycotoxins in Agriculture and Food Safety. Marcel Dekker, New York, pp 307–350

    Google Scholar 

  • Hocking AD (2003) Microbiological facts and fictions in grain storage. Stored Grain in Australia. CSIRO Stored Grain Research Laboratory, Canberra, pp 55–58

    Google Scholar 

  • Horn BW, Greene RL, Sobolev VS, Dorner JW, Powell JH (1996) Association of morphology and mycotoxin production with vegetative compatibility groups in Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamarii. Mycologia 88(4):574–587

    Article  CAS  Google Scholar 

  • Horn BW, Dorner JW (1999) Regional differences in production of aflatoxin B1 and cyclopiazonic acid by soil isolates of Aspergillus flavus along a transect within the United States. Appl Environ Microbiol 65:1444–1449

    CAS  PubMed  Google Scholar 

  • International Agency for Research on Cancer (IARC) (1993) Monograph on the Evaluation of Carcinogenic Risk to Humans, Vol. 56. IARC, Lyon, France, pp 257–263

  • Elegbede JA (1998) Legumes, Nutritional quality of plant foods. Post harvest Research Unit, Biochem, University of Benin, Benin City, pp 53–83

    Google Scholar 

  • Ito Y, Peterson SW, Wichlow DT, Goto T (2001) Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi. Mycol Res 105:233–239

    Article  Google Scholar 

  • Klich MA, Pitt JI (1988) Differentiation of Aspergillus flavus from Aspergillus parasiticus and other closely related species. Trans Brit Mycol Soc 91:99–108

    Article  Google Scholar 

  • Klich MA, Cary JW, Beltz SB, Bennett CA (2003) Phylogenetic and morphological analysis of Aspergillus ochraceoroseus. Mycologia 95:1252–1260

    Article  CAS  PubMed  Google Scholar 

  • Koehler PG (2003) Management of stored grain and peanut pests. Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida http://edis.ifas.ufl.edu

  • Lacey J, Magan N (1991) Fungi in cereal grain: Their occurrence and water and temperature relationship. In: Chelkowski J (ed) Cereal Grains Mycotoxins, Fungi and Quality in Drying and Storage. Elsevier, Amsterdam, pp 77–118

    Google Scholar 

  • Lacey J, Ramakrishna N, Hamer A, Magan N, Marfleet C (1991) Grain fungi. In: Arora DK (ed) Handbook of applied mycology: Foods and feeds. Marcel Dekker, New York, pp 12–77

    Google Scholar 

  • Lung-Shu L (1981) Stored grain mites in the work environment of farmers, Proceedings of the 6th International Congress of Acarology, vol. 2, Edinburgh, pp 1002-1005

  • Lynch RE, Wilson DM (1991) Enhanced infection of peanut, Arachis hypogaea L., seeds with Aspergillus flavus group fungi due to external scarification of peanut pods by the lesser cornstalk borer, Elasmopalpus lignosellus (Zeller). Peanut Sci 18:110–116

    Article  Google Scholar 

  • Magnoli C, Astoreca A, Ponsone L, Fernández-Juri MG, Chiacchiera S, Dalcero A (2006) Ochratoxin A and the occurrence of ochratoxin A-producing black aspergilla in stored peanut seed from Córdoba, Argentina. J Sci Food Agric 86:2369–2373

    Article  CAS  Google Scholar 

  • Nakai VK, Oliveira Rocha L, de Goncalez E, Fonseca H, Ortega EMM, Correa B (2008) Distribution of fungi and aflatoxinas in a stored peanut variety. Food Chem 106:285–290

    Article  CAS  Google Scholar 

  • Ory RL, Crippen KL, Lovegren NV (1992) Off-flavors in peanuts and peanut products. In: Charalambous G (ed) Off-flavors in foods and beverages. Elsevier, Amsterdam, pp 57–75

    Google Scholar 

  • Passone MA, Bluma R, Nesci A, Resnik S, Etcheverry MG (2008) Impact of food grade antioxidants on peanut pods and seeds mycoflora in storage system from Córdoba, Argentina. J Food Sci 28:550–566

    CAS  Google Scholar 

  • Pheloung P, Macbeth F (2002) Export inspection: adding value to Australia’s grain. In: Wright EJ, Banks HJ, Highley E (eds) Stored Grain in Australia 2000, Proceedings of the Australian Postharvest Technical Conference. Adelaide. CSIRO Stored Grain Research Laboratory, Canberra, Australia, pp 15–17

    Google Scholar 

  • Pildain MB, Vaamonde G, Cabral D (2004) Analysis of population structure of Aspergillus flavus from peanut based on vegetative compatibility, geographic origin, mycotoxin and sclerotia production. Int J Food Microbiol 93:31–40

    Article  CAS  PubMed  Google Scholar 

  • Pitt J (1988) A laboratory guide to common Penicillium species. CSIRO, Division of Food Research Sydney. Academic, Australia

    Google Scholar 

  • Pitt J, Hocking AD (1997) Fungi and Food Spoilage, vol II. Blackie, London

    Google Scholar 

  • PUCC (Pontificia Universidad Católica de Chile), Facultad de Agronomía e Ingeniería forestal (2007) Morfología e identificación de insectos www.puc.cl/sw_educ/agronomía/insectos/index.htm/

  • Rajendran S (2002) Postharvest pest losses. In: Pimentel D (ed) Encyclopedia of Pest Management. Marcel Dekker, New York, pp 654–656

    Google Scholar 

  • Rajendran S, Sriranjini V (2008) Plant products as fumigants for stored-product insect control. J Stored Prod Res 44:126–135

    Article  CAS  Google Scholar 

  • Riley RT, Norred WP, Bacon CW (1993) Fungal toxins in foods: Recent concerns. Annu Rev Nutr 13:167–189

    Article  CAS  PubMed  Google Scholar 

  • Rossetto CAV, Silva OF, Araújo AES (2005) Influéncia da calagem, da época de colheita e da secagem na incidéncia de fungos e aflatoxinas em graos de amendoim armazenados. Ciencia Rural 35(2):309–315

    CAS  Google Scholar 

  • SAGPyA (Secretaría de Agricultura, Ganadería, Pesca y Alimentos) (2005) Indicadores del sector manisero 2003/2004. Secretaría de Agricultura, Ganadería, Pesca y Alimentación, Buenos Aires, Argentina http://siiap.sagyp.mecon.ar

  • SAGPyA (Secretaría de Agricultura, Ganadería, Pesca y Alimentos) (2009) Estimaciones Agrícolas de Cereales, Dirección de Coordinación de Delegaciones, Buenos Aires, Argentina http://www.agrositio.com/vertext/vertext_print.asp?id=99638&se=1000

  • Samson RA, van Reenen-Hoekstra ES, Frisvad JC, Filtenborg O (2000) Introduction to Food and Airborne Fungi. Centraalbureau voor Schimmelcultures, Institute of the Royal Netherlands Academy of Arts and Sciences, The Netherlands

    Google Scholar 

  • Saint Geroges-Gridelet D (1984) Effects of dietary lipids on the population growth of Dermatophagoides pteronyssinus. Proceedings of 6th International Congress of Acarology, Chichester, Vol. 1, pp 351-357

  • Salunkhe JF, Adsule RN, Padule DN (1987) Occurrence of aflatoxin. In: Salunkhe JF, Adsule RN, Padule DN (eds) Aflatoxin in Foods and Feeds. Dahwan, New Delhi, pp 44–92

    Google Scholar 

  • Sanders TH, Vercellotti JR, Grimm DT (1993) Shelf-life peanuts and peanuts products. In: Charalambous G (ed) Shelf life stability of foods and beverages. Elsevie, Amsterdam, pp 289–309

    Google Scholar 

  • Trucksess MW, Stack ME, Nesheim S, Albert RH, Romer TR (1994) Multifunctional column coupled with liquid chromatography for determination of aflatoxins B1, B2, G1, G2 in corn, almonds, Brazil nuts, peanuts and pistachio nuts: collaborative study. J AOAC 6:1512–1521

    Google Scholar 

  • Van Egmond HP (2003) Mycotoxins and Regulations: an Update. Proceedings of the 2nd World Mycotoxin Forum, Nordwijk, The Netherlands

  • White NDG (1995) Insects, mites and insecticides in stored-grain ecosystems. In: Jayas P, White NDG, Muir WE (eds) stored-grain ecosystems. Marcel Dekker, New York, pp 123–167

    Google Scholar 

Download references

Acknowledgements

This work was carried out through grants from CONICET PIP 5822/05 and SECYT-UNRC 2007 to 2008.

Author information

Authors and Affiliations

  1. Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 km 601 (X5806JRA), Río Cuarto, Córdoba, Argentina

    Andrea Nesci, Analía Montemarani & Miriam Etcheverry

Authors
  1. Andrea Nesci
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Analía Montemarani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miriam Etcheverry
    View author publications

    You can also search for this author in PubMed Google Scholar

Consortia

Members of the Research Career of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

Corresponding author

Correspondence to Miriam Etcheverry.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nesci, A., Montemarani, A., Etcheverry, M. et al. Assessment of mycoflora and infestation of insects, vector of Aspergillus section Flavi, in stored peanut from Argentina. Mycotox Res 27, 5–12 (2011). https://doi.org/10.1007/s12550-010-0069-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12550-010-0069-0

Keywords

Search

Navigation