Abstract
Nine novel polymorphic microsatellite markers were isolated in the boring giant clam (Tridacna crocea). The number of alleles ranged from 7 to 21. The observed and expected heterozygosity varied from 0.400 to 1.000 and 0.727 to 0.932, respectively. No significant linkage disequilibrium between pairs of loci was found and eight of nine loci were in Hardy-Weinberg proportions. Cross-amplification in other tridacnid species showed that all loci could be successfully amplified in Tridacna maxima, five in T. squamosa, three in T. derasa, three in T. gigas, and three in T. costata. These markers are therefore potentially useful for studies on the genetic diversity and connectivity of giant clam populations in order to facilitate the spatial arrangement of marine protected areas (MPAs) and fisheries management for these species.
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Acknowledgements
We would like to thank to the institutions and individuals that have made our study possible: German Federal Ministry of Education and Research (BMBF) (grant no. 03F0390B and 03F0472B), which funded this study in the framework of the SPICE project (Science for the Protection of Indonesian Coastal Marine Ecosystems); China Scholarship Council (CSC) for the PhD scholarship to Min Hui; Wiebke Krämer (University of Bremen, Germany) for providing Symbiodinium cultures; Agus Nuryanto (Jenderal Soedirman University, Indonesia) and Janne Timm (University of Bremen, Germany) for support during collection of samples; colleagues from Hasanuddin University (Indonesia) for logistical support during field work; Dr. Aibin Zhan (University of Windsor, Canada) for helpful comments on the manuscript; Dr. Christoph Held (Alfred-Wegener-Institute for Polar and Marine Research, Germany) for laboratory support. The SPICE project is conducted and permitted under the governmental agreement between the BMBF and the Indonesian Ministry for Research and Technology (RISTEK), Indonesian Institute of Sciences (LIPI), Indonesian Ministry of Maritime Affairs and Fisheries (DKP), and Indonesian Agency for the Assessment and Application of Technology (BPPT).
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Hui, M., Kochzius, M. & Leese, F. Isolation and characterisation of nine microsatellite markers in the boring giant clam (Tridacna crocea) and cross-amplification in five other tridacnid species. Mar Biodiv 42, 285–287 (2012). https://doi.org/10.1007/s12526-011-0101-4
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DOI: https://doi.org/10.1007/s12526-011-0101-4