Skip to main content
SpringerLink
Log in

Alterations of the ovarian histomorphometry at pre-puberty in rat offspring from diabetic mothers

  • Original Article
  • Published:
Reproductive Medicine and Biology

Abstract

Purpose

Maternal diabetes leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure.

Methods

Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. Thirty days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological and cellular parameters were determined using histological and electron microscopy techniques.

Results

Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of the controls. The weight, volume and diameter of the ovary and the ovarian capsule thickness were inclined to decrease in ODM compared to that of controls. The number and diameter of primary, pre-antral and antral follicles were decreased in ovaries in the ODM. The electro-micrographs have demonstrated the organelle alterations in oocytes and granulosa cells that suggest the apoptosis progress and oxidative stress.

Conclusions

Maternal hyperglycaemia exhibited deleterious effects on the female reproductive system in the offspring.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Jones CW. Gestational diabetes and its impact on the neonate. Neonatal Netw. 2001;20:17–23.

    Article  PubMed  Google Scholar 

  2. Persson B, Hanson U. Neonatal morbidities in gestational diabetes mellitus. Diabetes Care. 1998;2:79–84.

    Google Scholar 

  3. Van Assche FA, Aerts L, De Prins FA. Degranulation of the insulin-producing B cells in an infant of a diabetic mother. Br J Obstet Gynecol. 1983;90:182–5.

    Article  Google Scholar 

  4. Ballester J, Muñoz MC, Domínguez J, Palomo MJ, Rivera M, Rigau T, Guinovart JJ, Rodríguez-Gil JE. Tungstate administration improves the sexual and reproductive function in female rats with streptozotocin-induced diabetes. Hum Reprod. 2007;22:2128–235.

    Article  PubMed  CAS  Google Scholar 

  5. Ahmed RG. Evolutionary interactions between diabetes and development. Diabetes Res Clin Pract. 2011;92:153–67.

    Article  PubMed  CAS  Google Scholar 

  6. Bestetti G, Locatelli V, Tirone F, Rossi GL, Müller EE. One month of streptozotocin-diabetes induces different neuroendocrine and morphological alterations in the hypothalamo-pituitary axis of male and female rats. Endocrinology. 1985;117:208–16.

    Article  PubMed  CAS  Google Scholar 

  7. Blades RA, Bryant KR, Whitehead SA. Feedback effects of steroids and gonadotrophin control in adult rats with streptozotocin-induced diabetes mellitus. Diabetologia. 1985;28:348–54.

    Article  PubMed  CAS  Google Scholar 

  8. Moley KH, Chi MM, Knudson CM, Korsmeyer SJ, Mueckler MM. Hyperglycemia induces apoptosis in pre-implantation embryos through cell death effector pathways. Nat Med. 1998;4:1421–4.

    Article  PubMed  CAS  Google Scholar 

  9. Goswami D, Conway GS. Premature ovarian failure. Hum Reprod Update. 2005;11:391–410.

    Article  PubMed  CAS  Google Scholar 

  10. Garris DR, Garris BL. Diabetes (db/db) mutation-induced ovarian involution: progressive hypercytolipidemia. Exp Biol Med (Maywood). 2003;228:1040–50.

    CAS  Google Scholar 

  11. Garris DR, Garris BL. Diabetes (db/db) mutation-induced female reproductive tract hypercytolipidemia: estrogenic restoration of utero-ovarian indices. Reprod Toxicol. 2004;18:641–51.

    Article  PubMed  CAS  Google Scholar 

  12. Lin S, Lin K, Li W, Zhou X, Huang T. Maternal diabetes increases apoptosis in mice oocytes, not 2-cell embryos. Endocrine. 2010;37:460–6.

    Article  PubMed  CAS  Google Scholar 

  13. Garris DR, Williams SK, West L. Morphometric evaluation of diabetes-associated ovarian atrophy in the C57BL/KsJ mouse: relationship to age and ovarian function. Anat Rec. 1985;211:434–43.

    Article  PubMed  CAS  Google Scholar 

  14. Jelodar G, Khaksar Z, Pourahmadi M. Endocrine profile and testicular histomorphometry in adult rat offspring of diabetic mothers. J Physiol Sci. 2009;59:377–82.

    Article  PubMed  CAS  Google Scholar 

  15. SzkuDelski T. The mechanism of Alloxan and Streptozotocin action in B Cells of the rat pancreas. Physiol Res. 2001; 50: 536–46.

    Google Scholar 

  16. Damas J, Hallet C, Lefebvre PJ. Changes in blood glucose and plasma insulin levels induced by bradykinin in anaesthetized rats. Br J Pharmacol. 2001;134:1312–8.

    Article  PubMed  CAS  Google Scholar 

  17. Turner CD, Bagnara JT. General endocrinology. Philadelphia: WB Saunders Company; 1971.

    Google Scholar 

  18. Elias H, Hyde DM. An elementary introduction to stereology (quantitative microscopy). Am J Anat. 1980;159:411–7.

    Article  Google Scholar 

  19. Luna LG. Manual of histologic staining methods of the Armed forces institute of pathology. USA: McGraw Hill; 1968.

    Google Scholar 

  20. Wrotniak BH, Shults J, Butts S, Stettler N. Gestational weight gain and risk of overweight in the offspring at age 7 y in a multicenter, multiethnic cohort study. Am J Clin Nutr. 2008;87:1818–24.

    PubMed  CAS  Google Scholar 

  21. Khaksar Z, Jelodar G, Hematian H. Cerebrum malformation in offspring of diabetic mothers. Comp Clin Pathol. 2011;10:1160–9.

    Google Scholar 

  22. Chang SA, Dale NA, Oley HK. Maternal diabetes adversely affects pre-ovulatory oocyte maturation, development, and granulosa cell apoptosis. Endocrinology. 2005;146:2445–53.

    Article  PubMed  CAS  Google Scholar 

  23. Wang Q, Frolova AI, Purcell S, Adastra K, Schoeller E, Chi MM, Schedl T, Moley KH. Mitochondrial dysfunction and apoptosis in cumulus cells of type I diabetic mice. PLoS ONE. 2010;5:e15901.

    Article  PubMed  CAS  Google Scholar 

  24. Sugiura K, Eppig JJ. Control of metabolic cooperativity between oocytes and their companion granulosa cells by mouse oocytes. Reprod Fertil Dev. 2005;17:667–74.

    Article  PubMed  CAS  Google Scholar 

  25. Ishikawa S, Bensaoula T, Uga S, Mukuno K. Electron-microscopic study of iris nerves and muscles in diabetes. Ophthalmologica. 1985;191:172–83.

    Article  PubMed  CAS  Google Scholar 

  26. Schramm E, Wagner M, Nellessen U, Inselmann G. Ultrastructural changes of human cardiac atrial nerve endings in diabetes mellitus. Eur J Clin Invest. 2000;30:311–6.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to express their thanks to Mr Mogheisi, Mrs Ahmadi and Mrs Shams for their technical assistance.

Conflict of interest

We have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Zabihollah Khaksar & Gholamali Jelodar

  2. Department of Veterinary Sciences, Yasooj Branch, Islamic Azad University, Yasooj, Iran

    Hooman Hematian

  3. Department of Basic Sciences, Jahrom Medical University, Jahrom, Iran

    Mohamad Poorahmadi

Authors
  1. Zabihollah Khaksar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholamali Jelodar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hooman Hematian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohamad Poorahmadi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hooman Hematian.

Electronic supplementary material

About this article

Cite this article

Khaksar, Z., Jelodar, G., Hematian, H. et al. Alterations of the ovarian histomorphometry at pre-puberty in rat offspring from diabetic mothers. Reprod Med Biol 12, 173–178 (2013). https://doi.org/10.1007/s12522-013-0151-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12522-013-0151-3

Keywords

Search

Navigation