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Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

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Reproductive Medicine and Biology

Abstract

Purpose

Examination of the mitochondrial mRNA expression in granulosa cells from an unspecified population of infertile patients to evaluate whether recombinant follicle stimulating hormone (recFSH) is more effective in producing higher quality embryo rates compared with human menopausal gonadotropin (hMG).

Method

Thirty-nine patients who underwent the in vitro fertilization and embryo transfer program were retrospectively examined. Patients were administered recFSH (n = 18) or hMG (n = 20) in a long protocol where GnRH agonist was used. Granulosa cells were obtained during oocyte retrieval and examined for mitochondria mRNA expression ratio against GAPDH. Expressions of mitochondria mRNA were evaluated by real-time PCR analysis.

Results

The high-quality embryo rate in the hMG cycle was higher than in the recFSH cycle, and the total dose of hMG showed a positive correlation with the expression level of mitochondrial genes in granulosa cells. Moreover, mitochondria mRNA expression was higher in the hMG cycle than in the recFSH cycle.

Conclusions

Compared with recFSH, hMG induces a higher mitochondrial gene expression ratio in granulosa cells at the time of oocyte retrieval and, therefore, may lead to higher quality embryo rates.

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Acknowledgment

We have no conflict of interest.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka, 569-8686, Japan

    Natsuho Saito, Yoshiki Yamashita, Yoshihiro Ono, Yoko Higuchi, Atsushi Hayashi, Yoko Yoshida, Hikaru Yamamoto, Sachiko Kawabe, Mika Kamada, Yoshito Terai & Masahide Ohmichi

Authors
  1. Natsuho Saito
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  2. Yoshiki Yamashita
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  3. Yoshihiro Ono
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  4. Yoko Higuchi
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  5. Atsushi Hayashi
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  6. Yoko Yoshida
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  7. Hikaru Yamamoto
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  8. Sachiko Kawabe
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  9. Mika Kamada
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  10. Yoshito Terai
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  11. Masahide Ohmichi
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Corresponding author

Correspondence to Yoshiki Yamashita.

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Saito, N., Yamashita, Y., Ono, Y. et al. Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer. Reprod Med Biol 12, 99–104 (2013). https://doi.org/10.1007/s12522-013-0147-z

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  • DOI: https://doi.org/10.1007/s12522-013-0147-z

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