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Degradation of Deoxynivalenol by Atmospheric-Pressure Cold Plasma and Sequential Treatments with Heat and UV Light

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Abstract

Atmospheric-pressure cold plasma (ACP) technology is a novel non-thermal method with the potential to be used as a post-harvest treatment for mycotoxin mitigation. Deoxynivalenol (DON), a mycotoxin that occurs predominantly in grains, is a threat to human health and to the agricultural economy worldwide. In this study, the effects of the treatment time, and the presence of ACN/water on the DON degradation efficacy during ACP treatment were evaluated. ACP treatment for 5 min resulted in 100% degradation in DON in solution (20 μg/ml ACN/water (20/80, v/v)), while the DON degradation was only 75.9% after 60 min of treatment, in dry condition (2 μg DON). Thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) were used to determine structural changes in DON after ACP treatment. FTIR analysis revealed major changes in DON functional groups after ACP treatment in solution form. Sequential treatments involving ACP and heat treatment at 80 °C and ACP and light-emitting diode (LED) treatments were tested to reduce DON concentrations in dry form. Heat treatment alone was not effective in reducing DON, while sequential treatment of 30-min ACP with 25-min heat reduced DON by 64.6%, which was not significantly different from the DON reduction (63%) by 30-min ACP alone. LED treatments for 30 and 60 min reduced DON concentrations by 10.7% and 36.7%, respectively; while sequential treatment of 30-min ACP with 30-min LED reduced DON by 68.8%. This study shows that the ACP technology has the potential to degrade DON.

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Funding

We received funding support from Alberta Agriculture and Forestry, Alberta Canola Producers Commission, and Natural Sciences and Engineering Research Council (grant nos. ACPC103MD2018, 2018F180R, RGPIN-2017-05051, CRDPJ532306). Ehsan Feizollahi received scholarship from Brewing and Malting Barley Research Institute.

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Authors and Affiliations

  1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Ehsan Feizollahi, Muhammad Arshad, Barun Yadav, Aman Ullah & M. S. Roopesh

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  1. Ehsan Feizollahi
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  2. Muhammad Arshad
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  3. Barun Yadav
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  4. Aman Ullah
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  5. M. S. Roopesh
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BY methodology, investigation, review and editing; EF conceptualization, methodology, software, validation, investigation, writing-original draft, review and editing; MA methodology, investigation, review and editing; AU methodology, investigation, review and editing; MSR conceptualization, methodology, investigation, review and editing, supervision, funding acquisition.

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Correspondence to M. S. Roopesh.

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Feizollahi, E., Arshad, M., Yadav, B. et al. Degradation of Deoxynivalenol by Atmospheric-Pressure Cold Plasma and Sequential Treatments with Heat and UV Light. Food Eng Rev 13, 696–705 (2021). https://doi.org/10.1007/s12393-020-09241-0

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