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Ethylene was Involved in Ca2+-Regulated Na+ Homeostasis, Na+ Transport and Cell Ultrastructure During Adventitious Rooting in Cucumber Explants Under Salt Stress

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Abstract

Salt stress as one of main abiotic stresses damaged plant growth and development. In this study, the effects of ethylene on Ca2+-regulated rooting index, Na+ and K+ concentration, Na+ transportation and cell ultrastructure during adventitious rooting in cucumber (Cucumis sativus L.) under salt stress were investigated. The results showed that ethylene might play a positive role in Ca2+-regulated rooting index during adventitious rooting under salt stress. Moreover, CaCl2 treatment significantly decreased the intracellular Na+ level but increased K+ content of cucumber explants under salt stress. However, the Na+ and K+ concentration which treated with Ca2+ treatment was significantly influenced by ethylene inhibitors under salt stress. Our results also showed that ethylene might be involved in Ca2+-affected cell ultrastructure of cucumber explants under salt condition. Further analysis showed that ethylene might be responsible for Ca2+-regulated Na+ transport through enhancing the transcriptional and protein levels of Na+/H+ antiporter as well as H+-ATPase for alleviating the negative effects of NaCl stress. Therefore, ethylene might act as a downstream signaling molecule of Ca2+ to maintain Na+ and K+ homeostasis via regulating Na+ transport and preserve the integrity of cellular ultrastructure of cucumber explant during adventitious rooting under salt condition.

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Funding

This work was supported by the National key research and development projects (2018YFD0201205), the National Natural Science Foundation of China (No. 31660584), China Agriculture Research System (CARS-23-C-07), Gansu Province Science and Technology Key Project Fund (No. 17ZD2NA015).

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Authors and Affiliations

  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China

    Jian Yu, Jihua Yu, Weibiao Liao, Jianming Xie, Lijuan Niu, Guobin Zhang, Jian Lv, Xuemei Xiao & Yue Wu

  2. Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China

    Jihua Yu

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  1. Jian Yu
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  2. Jihua Yu
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Correspondence to Jihua Yu.

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Yu, J., Yu, J., Liao, W. et al. Ethylene was Involved in Ca2+-Regulated Na+ Homeostasis, Na+ Transport and Cell Ultrastructure During Adventitious Rooting in Cucumber Explants Under Salt Stress. J. Plant Biol. 63, 311–320 (2020). https://doi.org/10.1007/s12374-020-09254-6

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