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Fusion insect-resistant gene mediated by matrix attachment region (MAR) sequence in transgenic sugarcane

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Abstract

With the embryogenic calli of sugarcane variety ROC25 as the acceptors, a genetic transformation system mediated by Agrobacterium tumefaciens was established and the fusion insect-resistant gene (constructed by Amaranthus viridis L. agglutinin gene and soybean Kunitz tripsin inhibitor, AVAc-SKTI) transformed plants were acquired. The plant expression vector was constructed with the fused gene which was flanked on both sides of the MARs. The plant vectors were transformed into the embryogenic calli with the plant expression vector without MAR control. In the genetic transformation of sugarcane, the vector ligated with MARs in the same direction on both sides of the fused gene could increase the number of transgenic lines, which was 55.56% higher than that of the control. Moreover, the number of transgenic plants regenerated from each resistant callus increased by 26.43%. The AVAc-SKTI fusion insect-resistant gene transformed plants would improve the resistance of sugarcane plants to the attacks of borer (Diatraea saccharalis) and aphid (Ceratovacuna lanigera Zehnther).

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Authors and Affiliations

  1. Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Science, Nanning, Guangxi, 530007, China

    Zhi-Nian Deng, Yuan-Wen Wei, Wei-Li Lü & Yang-Rui Li

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  1. Zhi-Nian Deng
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  2. Yuan-Wen Wei
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  3. Wei-Li Lü
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  4. Yang-Rui Li
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Correspondence to Yang-Rui Li.

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Deng, ZN., Wei, YW., Lü, WL. et al. Fusion insect-resistant gene mediated by matrix attachment region (MAR) sequence in transgenic sugarcane. Sugar Tech 10, 87–90 (2008). https://doi.org/10.1007/s12355-008-0015-z

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  • DOI: https://doi.org/10.1007/s12355-008-0015-z

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