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Reproducibility and accuracy of non-invasive measurement of infarct size in mice with high-resolution PET/CT

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Journal of Nuclear Cardiology Aims and scope

Abstract

Background

This study assessed the reproducibility and accuracy of 2-deoxy-2[18F]fluoro-d-glucose (18F-FDG) for non-invasive quantification of myocardial infarct size in mice by a high-resolution positron emission tomography (PET)/computed tomography (CT) system.

Methods and Results

Mice were studied by 18F-FDG PET/CT 1 week after induction of myocardial infarction by permanent coronary occlusion or sham procedure. In a subset of mice, PET/CT was repeated 2 days apart to assess the reproducibility of infarct size measurements. Histological analysis was used as reference method to validate imaging data. The average difference in infarct size measurements between the first and the second study was −0.42% ± 2.07% (95% confidence interval −2.6 to 1.75) with a repeatability coefficient of 4.05%. At Bland-Altman analysis, the lower and upper limits of agreement between the two repeated studies were −4.46% and 3.63%, respectively, and no correlation between difference and mean was found (P = .89). The concordance correlation coefficient was 0.99 (P < .001) and the intraclass coefficient of correlation was 0.99. A high correlation between PET/CT and histology was found for measurement of infarct size (P < .001). Using Bland-Altman analysis, the mean difference in infarct size measurement (PET/CT minus histology) was 1.9% (95% confidence interval 0.94% to 2.86%).

Conclusions

In a mice model of permanent coronary occlusion non-invasive measurement of infarct size with high-resolution 18F-FDG, PET/CT has excellent reproducibility and accuracy. These findings support the use of this methodology in serial studies.

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Acknowledgment

The authors have indicated that they have no financial conflicts of interest.

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Authors and Affiliations

  1. Department of Biomorphological and Functional Sciences, University Federico II, Via Pansini 5, 80131, Naples, Italy

    Adelaide Greco DVM, PhD, Sara Gargiulo DVM, PhD, Arturo Brunetti MD & Alberto Cuocolo MD

  2. CEINGE Scarl, Naples, Italy

    Adelaide Greco DVM, PhD, Sara Gargiulo DVM, PhD & Arturo Brunetti MD

  3. Department of Internal Medicine, Cardiovascular and Immunological Sciences, University Federico II, Naples, Italy

    Maria Piera Petretta MD, Giovanni Esposito MD & Mario Petretta MD

  4. Institute of Biostructures and Bioimages of National Council of Research, Naples, Italy

    Michele Larobina PhD, Mariarosaria Panico PhD, Arturo Brunetti MD & Alberto Cuocolo MD

  5. Nuklearmedizinische Klinik und Poliklinik, Technischen Universität München, Munich, Germany

    Stephan G. Nekolla PhD

Authors
  1. Adelaide Greco DVM, PhD
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  2. Maria Piera Petretta MD
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  3. Michele Larobina PhD
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  4. Sara Gargiulo DVM, PhD
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  5. Mariarosaria Panico PhD
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  6. Stephan G. Nekolla PhD
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  7. Giovanni Esposito MD
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  8. Mario Petretta MD
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  9. Arturo Brunetti MD
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  10. Alberto Cuocolo MD
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Corresponding author

Correspondence to Alberto Cuocolo MD.

Additional information

Adelaide Greco and Maria Piera Petretta contributed equally to this work.

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Greco, A., Petretta, M.P., Larobina, M. et al. Reproducibility and accuracy of non-invasive measurement of infarct size in mice with high-resolution PET/CT. J. Nucl. Cardiol. 19, 492–499 (2012). https://doi.org/10.1007/s12350-012-9538-5

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  • DOI: https://doi.org/10.1007/s12350-012-9538-5

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