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Macrophage Infiltration in Tumor Stroma is Related to Tumor Cell Expression of CD163 in Colorectal Cancer

  • Original Paper
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Cancer Microenvironment

Abstract

The scavenger receptor, CD163, is a macrophage-specific marker. Recent studies have shown that CD163 expression in breast and rectal cancer cells is associated with poor prognosis. This study was conducted to evaluate the relationship between CD163 expression as a macrophage trait in cancer cells, and macrophage infiltration and its clinical significance in colorectal cancer. Immunostaining of CD163 and macrophage infiltration were evaluated in paraffin-embedded specimens, earlier analyzed for CD31, D2-40 and S-phase fraction, from primary tumors and normal colorectal mucosa of 75 patients with colorectal carcinoma. The outcomes were analyzed in relation to clinical-pathological data. CD163 expression was positive in cancer cells in 20 % of colorectal cancer patients and was related to advanced tumor stages (P = 0.008) and unfavorable prognosis (p = 0.001). High macrophage infiltration was related to shorter survival and positive CD163 expression in tumor cells. The prognostic impact of macrophage infiltration was independent of tumor stage and CD163 expression in cancer cells (p = 0.034). The expression of macrophage phenotype in colorectal cancer cells is associated with macrophage density in tumor stroma and lower survival rates. Macrophage infiltration has an independent prognostic impact on mortality in colorectal cancer. In accordance with previous experimental studies, these findings provide new insights into the role of macrophages in colorectal cancer.

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Acknowledgements

The authors are indebted to members of the South-East Sweden Colorectal Cancer Group for providing us with clinical data. Thanks are due to Mrs. Birgitta Frånlund for valuable help with the immunohistochemistry. The work was supported by The Health Research Council in the South-East of Sweden.

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County Council of Östergötland, Sweden.

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All authors have no conflicts of interest.

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Authors and Affiliations

  1. Division of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Science, Linköping University, County Council of Östergötland, Linköping, Sweden

    Ivan Shabo & Joar Svanvik

  2. Division of Pathology, Department of Clinical and Experimental Medicine, Faculty of Health Science, Linköping University, County Council of Östergötland, Linköping, Sweden

    Hans Olsson & Rihab Elkarim

  3. Department of Clinical and Experimental Medicine, Faculty of Health Science, Linköping University, section of endocrine surgery, Linköping, Sweden

    Ivan Shabo

  4. Department of surgery, County Council of Östergötland, Linköping, Sweden

    Ivan Shabo

  5. Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Science, Linköping University, County Council of Östergötland, Linköping, Sweden

    Xiao-Feng Sun & Xiao-Feng Sun

  6. Division. of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Science, section of endocrine surgery, department of surgery, County Council of Östergötland, Linköping University, SE 581 85, Linköping, Sweden

    Ivan Shabo

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  1. Ivan Shabo
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Correspondence to Ivan Shabo.

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Shabo, I., Olsson, H., Elkarim, R. et al. Macrophage Infiltration in Tumor Stroma is Related to Tumor Cell Expression of CD163 in Colorectal Cancer. Cancer Microenvironment 7, 61–69 (2014). https://doi.org/10.1007/s12307-014-0145-7

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