Skip to main content
SpringerLink
Log in

Polymorphism analysis in advanced mutant population of oat (Avena sativa L.) using ISSR markers

  • Research Article
  • Published:
Physiology and Molecular Biology of Plants Aims and scope Submit manuscript

Abstract

Present investigation was carried out to evaluate genetic diversity among 38 M6 population of oat cv. JO-1. To validate the observed morpho-physiological variations, these lines were analyzed with 21 ISSR primers. A total of 132 loci were amplified by these 21 ISSR markers and 116 loci were found to be polymorphic (87.87 %). The genetic similarity coefficient values among 39 oat genotypes based on ISSR analysis ranged from 0.305 to 0.957. The cluster analysis divided the oat genotypes into two groups. Mutants JMO 81 and JMO 82 were found to be most divergent, hence can be used as parents in breeding program for the development of superior cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Atak C, Celik O, Acik L (2011) Genetic analysis of Rhododendron mutants using random amplified polymorphic DNA (RAPD). Pak J Bot 43(2):1173–1182

    CAS  Google Scholar 

  • Babaei A, Nematzadeh GA, Avagyan V, Hamidreza S, Petrodi H (2010) Radio sensitivity studies of morpho- physiological characteristics in some Iranian rice varieties (Oryza sativa L.) in M1 generation. Afr J Agric Res 5(16):2124–2130

    Google Scholar 

  • Chao S, Zhang W, Dubcovsky J, Sorrells M (2007) Evaluation of genetic diversity and genome-wide linkage disequilibrium among US wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Sci 47(3):1018–1030

    Article  CAS  Google Scholar 

  • De Souza VQ, Pereira AS, Kopp MM, Coimbra JLM, Carvalho FIF, Luz VK, Oliveira AC (2005) Genetic dissimilarity in oat (Avena sativa L.) tolerant and sensitive mutants to organic acids. Bragantia 64(4):569–575

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12(1):13–15

    Google Scholar 

  • Edmé SJ, Comstock JC, Miller JD, Tai PYP (2005) Determination of DNA content and genome size in sugarcane. J Amer Soc Sugarcane Tech 25:1–16

  • Garris AJ, Tai TH, Coburn J, Kresovich S, McCouch SR (2005) Genetic structure and diversity in Oryza sativa L. Genetics 169(3):1631–1638

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Govindaraj M, Vetriventhan M, Srinivasan M (2015) Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives. Genetics Res Int. doi:10.1155/2015/431487

    Google Scholar 

  • Gowda MVC, Nadaf HL, Sheshagiri R (1996) The role of mutations in intraspecific differentiation of ground nut (Arachis hypogaea L.). Euphytica 90:105–113

    Google Scholar 

  • Hautea DM, Molina GC, Balatero CH, Coronado NB, Perez EB, Alvarez MTH, Canama AO, Akuba RH, Quilloy RB, Frankie RB, Caspillo CS (2004) Analysis of induced mutants of Philippine with molecular markers. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology and induced mutations. http://www.fao.org/docrep/007/ae216e/ae216e07.htm#bm07

  • Khan IA, Bibi S, Yasmin S, Khatri A, Seema N, Afghan S (2010) Genetic variability in mutated population of sugarcane clone nia-98 through molecular markers (RAPD and TRAP). Pak J Bot 42(1):605–614

    CAS  Google Scholar 

  • Miri SM, Mousavi A, Naghavi MR, Khiabani BN (2014) Molecular analysis of musa mutants resistant to salinity by microsatellite markers. Trakia J Sci 2:114–120

    Google Scholar 

  • Mudibu J, Nkongolo KKC, Mehes-Smith M, Kalonji-Mbuyi A (2011) Genetic analysis of soybean genetic pool using ISSR merker: effect of gamma radiation on genetic variability. Int J Plant Breed Genet 5(3):235–245

    Article  Google Scholar 

  • Oliver RE, Obert DE, Hu G, Bonman JM, Jackson EW (2010) Development of oat-based markers from barley and wheat microsatellites. Genome 6:458-471

  • Rohlf FJ (2000) NTSYSpc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, NY

  • Roldan-Ruiz I, Dendauw J, Vanbockstaele E, Depicker A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6:125–134

    Article  CAS  Google Scholar 

  • Ruwali Y, Singh K, Kumar S, Kumar L (2013) Molecular diversity analysis in selected fodder and dual purpose oat (Avena sativa L.) genotypes by using random amplified polymorphic DNA (RAPD). Afr J Biotechnol 12:3425–3429

    CAS  Google Scholar 

  • Tanhuanpaa P, Kalendar R, Laurila J, Schulman AH, Manninen O, Kiviharju E (2006) Generation of SNP markers for short straw in oat (Avena sativa L.). Genome 49:282–287

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, 482004, India

    Pawan Sharma, Sharad Tiwari & Niraj Tripathi

  2. Department of Agronomy, Jawaharlal Nehru Agricultural University, Jabalpur, 482004, India

    Anoop K. Mehta

Authors
  1. Pawan Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sharad Tiwari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Niraj Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anoop K. Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sharad Tiwari.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharma, P., Tiwari, S., Tripathi, N. et al. Polymorphism analysis in advanced mutant population of oat (Avena sativa L.) using ISSR markers. Physiol Mol Biol Plants 22, 115–120 (2016). https://doi.org/10.1007/s12298-015-0333-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12298-015-0333-z

Keywords

Search

Navigation