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Assessment of genetic diversity in rice [Oryza sativa L.] germplasm based on agro-morphology traits and zinc-iron content for crop improvement

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Abstract

Genetic resources of landraces (84 cultivars) were collected from various agro-ecological regions of West Bengal and adjoining areas and characterized based on qualitative and quantitative agro-morphological descriptors along with zinc (Zn) and iron (Fe) content. The DUS protocol was employed to study 16 agro-morphological passport data such as: vegetative data (anthocyanin pigmentation, plant habit), reproductive data (flag leaf attitude, stigma colour, panicle attitude), including eight grain quality traits: grain length, grain width, 1000 grains weight, kernel length, kernel breadth etc. Highest seed weight was found in cultivar Khechri (32.04 g/1000 seeds), collected from Sundarban and least seed weight was 9.6 g/1000seeds in Katharibhog. Maturity duration was found very short (<100 days) in Jumla Marshi (97 days) collected from world’s coldest rice growing area, Jumla, Nepal. Penultimate leaves breadth was observed broad (>2 cm) in one cultivar Jungli (2.3 cm). Seeds per panicle were 180 in Chinisakkar (medium range), 177 in Dudheswar, and 151 in Ladua. Flag leaf was found in erect condition in late observation in Dudheswar, Enda and Ghiosh. Seventeen cultivars were grouped in the aromatic rice category out of total 84 local landraces. Twenty-one cultivars were with awn, whose length ranges from 1.6 mm (Anandi) to 22.5 mm (Tulaipanji). Kernel colour varies from red, yellowish, brownish, creamy white to white. Kernel length varies from 4 mm to 8 mm and breadth 1.90 mm to 3 mm. Kernel length/breadth ration varied from 1.6 to 3.9. Highest ratio of L/B was found in Pusa Basmati 1(3.9) and lowest in Dudhey (1.6). Elongation ration was highest in Kalokure (2.07) and lowest in Phoolpakri (0.62). Nutritional values of mineral contents of iron (Fe) and zinc (Zn) were estimated in all cultivars by Atomic Absorption Spectrophotometic method. Iron concentration varies from 0.25 μg/g to 34.8 μg/g and zinc from 0.85 μg/g to 195.3 μg/g in the landraces. Highest iron containing rice was Swetonunia with 34.8 μg/g and highest amount of Zn was found in Nepali Kalam which was 195.3 μg/g. Anaerobic germination (AG) was observed in 18 cultivars among 84 land races (viz. Jungli, Kumrogore, Dudheshwar, Rambhog and Tulsi etc.), the trait is highly desired by the rice breeder for the introgression of this gene (QTL) to the HYV for direct seeding in the field for saving labour cost and reduced maturity time. Dendrogram showed genetic diversity among 84 landraces by grouping them into five major clusters. All the descriptors evaluated in this study have showed that there is enough genetic diversity among landraces and this information can be helpful to the breeders to choose the right parent for crop improvement.

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Acknowledgments

We are thankful to the authority of the University of North Bengal [Ref. No. 4031/R-2013 dt. 13.08.2013] for financial support and providing experimental rice field with irrigation system in the NBU Campus to carry out this research work. Thanks are due to Dr. B. Sinha, Department of Chemistry, NBU for helping in Atomic Absorption Spectrophotometry.

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  1. Plant Genetics & Tissue Culture Laboratory, DRS Department of Botany, University of North Bengal, PO-NBU, Siliguri, 734013, WB, India

    Subhas Chandra Roy & B. D. Sharma

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  1. Subhas Chandra Roy
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  2. B. D. Sharma
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Correspondence to Subhas Chandra Roy.

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Roy, S.C., Sharma, B.D. Assessment of genetic diversity in rice [Oryza sativa L.] germplasm based on agro-morphology traits and zinc-iron content for crop improvement. Physiol Mol Biol Plants 20, 209–224 (2014). https://doi.org/10.1007/s12298-014-0221-y

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  • DOI: https://doi.org/10.1007/s12298-014-0221-y

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