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Influence of organic supplements on production of shoot and callus biomass and accumulation of bacoside in Bacopa monniera (L.) Pennell.

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Abstract

Production of valuable secondary metabolites through plant cell or organ culture is the best suited alternative to extraction of whole plant material and to increase production of secondary metabolites in in-vitro systems, feeding precursor or intermediate metabolites is an obvious and popular approach. The present investigation was aimed to study the influence of feeding of organic supplements, glycine (0–125 μM), ferulic acid (0–200 μM), phenylalanine (0–200 μM), α-ketoglutaric acid (0–200 μM) and pyruvic acid (0–200 μM) on production of bacoside-A (a triterpenoid type secondary metabolite responsible for cognition effects) in shoot and callus biomass of Bacopa monniera (L.) Pennell. The shoots were raised in liquid Murashige and Skoog’s (MS) medium fortified with 5 μM 6-benzyladenine (BA) and callus biomass on agar solidified MS medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4 -D) in conjunction with 5 μM 1-napthaleneacetic acid (NAA). Among the organic supplements used, 100 μM pyruvic acid effectively enhanced the production of bacoside-A in shoot as well as callus biomass. The bacoside-A content in in-vitro raised shoot biomass was 4.0 and 1.2 times higher as compared to control and shoot biomass of naturally grown plants respectively. Inclusion of pyruvic acid in MS medium for in-vitro shoot cultures of B. monniera, can be adapted for enhanced production of bacoside-A.

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Authors and Affiliations

  1. Department of Botany, University of Pune, Pune, 411 007, India

    Anuradha Parale & Tukaram Nikam

  2. Post Graduate Research Centre, Department of Botany, Modern College of Arts, Science and Commerce, Shivajinagar, Pune, 411 005, India

    Rajkumar Barmukh

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  1. Anuradha Parale
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  2. Rajkumar Barmukh
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  3. Tukaram Nikam
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Correspondence to Tukaram Nikam.

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Parale, A., Barmukh, R. & Nikam, T. Influence of organic supplements on production of shoot and callus biomass and accumulation of bacoside in Bacopa monniera (L.) Pennell.. Physiol Mol Biol Plants 16, 167–175 (2010). https://doi.org/10.1007/s12298-010-0018-6

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