Abstract
Effect of salinity (NaCl, 100 mM) on growth, nitrate reductase (NR) and glutamine synthetase (GS) activities, and uptake of NH4 + was studied in the wild type (WT) and the NaCl-tolerant mutant type (MT) of cyanobacterium Anabaena doliolum. Results obtained in the presence of salt showed significant reduction in the growth rate of both WT and MT cells of A. doliolum by about 77.8 and 40 %, respectively over without NaCl. Similarly rate of NR activity in both WT and MT strains was reduced by 45.5 and 44.5 %, respectively. On the contrary, rate of GS activity of both the WT and MT strains in the presence 100 mM of NaCl increased by 34 and 159 %, respectively. The results of this study indicate that tolerance to NaCl in A. doliolum is more dependent on NH4 + assimilation rather than on nitrate assimilation in relation to N-metabolism. The increased GS activity in MT cells of the cyanobacterium is possibly because of high rate of energy dependent NH4 + uptake.
Abbreviations
- ADP:
-
Adenosine di-phosphate
- GS:
-
Glutamine synthetase
- NR:
-
Nitrate reductase
- NTG:
-
N′methyl-N-nitro-N-nitrosoguanidine
- CCCP:
-
Carbonyl. cyanide m-chlorophenyl hydrazone
- DNP:
-
2, 4-Dinitrophenol
- WT:
-
Wild type
- MT:
-
Mutant type
References
Abdolzadeh A, Shima K, Lambers H and Chiba K (2008). Change in uptake, transport and accumulation of ions in Nerium oleander (rosebay) as affected by different nitrogen sources and salinity. Ann. Bot., 102: 735–746.
Apte SK and Bhagwat AA (1989). Salinity stress induced protein in two N2-fixing Anabaena strains differentially tolerant to salt. J. Bacteriol., 171: 909–915.
Aslam M, Huffaker RC and Rains DW (1984). Early effects of salinity on nitrate assmilation in barley seedlings. Plant Physiol., 76: 321–325.
Berteli F, Corrales E, Guerrero C, Ariza MJ, Pliego F and Valpuesta V (1995). Salt stress increases ferredoxin dependent glutamate synthase activity and protein level in the leaves of tomato. Plant Physiol., 93: 259–264.
Bhargava S and Singh K (2006). Differential response of NaCl-resistant mutants of the cyanobacterium Nostoc moscurum to salinity and osmotic stresses. World J. Microb. Biotech., 22: 783–789.
Binzel ML, Hess FD, Bressan RA and Hasegawa PM (1988). Intracellular compartmentation of ions in salt adapted tobacco cells. Plant Physiol., 86: 607–614.
Egamberdieva D, Kamilova F, Validov S, Gafurova L, Kucharova Z and Lugtenberg B (2008). High incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in Uzbekistan. Env. Microbiol., 10: 1–9.
Gazzarrini S, Lejay L, Gojon A, Ninnemann O, Formmer WB and Von-Woren N (1999). Three functional transporters for constitutive, diurnally regulated and starvation-induced uptake of ammonium into Arabidopsis roots. Plant Cell 11: 937–948.
Golden SS (1958). Mutagenesis of cyanobacteria by classical and gene transfer-based method. Methods Enzymol., 167: 714–727.
Herbert D, Phipps PJ and Strange RE (1971). Chemical analysis of microbial cells. In: Methods in Microbiology (Eds. Norris JR and Ribbons DW), Academic Press, London, pp. 209–234.
Karpel R, Alon T, Glaser G, Schuldiner S and Padan E (1991). Expression of a sodium proton antiporter (Nha A) in Escherichia coli is inducing by Na+ and Li+ ions. J. Biol. Chem., 266: 21753–21759.
Lauchli A (1990). Calcium, salinity and the plasma membrane. In: Calcium in Plant Growth and Development (Eds Leonard RT and Hepler PK), Am. Soc. Plant Physiol., Rockville, MD., pp. 26–35.
Li W, Wang Y, Okamoto M, Olive F, Crawford NM, Siddiqi MY and Glass ADM (2007). Dissection of the AtNTR2.1: AtNTR2.2 inducible high affinity nitrate transporter gene cluster. Plant Physiol., 143: 425–433.
Loque DS, Lalonde S, Looger LL, Von-Wiren N and Frommer WB (2007). A cytosolic trans-activation domain essential for ammonium uptake. Nature 446: 195–198.
Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ (1951). Protein measurements by Folin-phenol reagent. J. Biol. Chem., 193: 266–275.
Manazano C, Candau P, Gomez-Moreno C, Relimpio AM and Losada M (1976). Ferredoxin-dependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans. Mol. Cell Biochem., 10: 161–169.
Martinez V and Cerda A (1989). Nitrate reductase activity in tomato amd cucumber leaves as influenced by NaCl and N2 source. J. Plant Nutri., 12: 1335–1350.
Masclaux-Daubress C, Resisdorf-cren M, Pageau K, Lelandais M, Grandjean O, Kronberger J Valadier MH, Feraud M, Louglent T and Suzuki A (2006). Glutamine synthetase-glutamate synthetase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco. Plant Physiol., 140: 444–456.
Niu X, Bressan RA, Hasegawa PM and Pardo JM (1995). Ion homeostasis in NaCl stress environments. Plant Physiol., 109: 735–742.
Reed RH, Richardson DL and Stewart WDP (1985). Na+ uptake and extrusion in cyanobacterium Synechocystis PCC 6714 in response to hypersaline treatment: Evidence for transient chages in plasmalemma Na+ permeability. Biochem. Biophys. Acta., 814: 347–355.
Salehi M, Salehi F, Poustini K and Sharifabad HH (2008). The effect of salinity on the nitrogen fixation in 4 cultivars of Medicago sativa l. in the seedling emergence stage. Res. J. Ag. Biological Sci., 4: 413–415.
Shapiro BM and Stadtman ER (1970). Glutamine Synthetase. In: Methods in Enzymology (Eds. Taber H and Tabor CW), Academic Press, New York, London, pp. 910–922.
Singh Devendra P and Kshatriya Kavita (2002). Characterization of Salinity-Tolerant Mutant of Anabaena doliolum Exhibiting Multiple Stress Tolerance. Curr. Microbiol., 45:165–170.
Soltani N, Zarrini G, Ghasemi Y, Shokravish S and Baftechchi L (2007). Characterization of a soil cyanobacterium Fischerella sp. FS18 under NaCl stress. J. Biol. Sci., 7: 931–936.
Waditee R, Hibino T, Tanaka Y, Nakamura T, Incharoensakdi A and Takabe T (2001). Halotolerant cyanobacterium Aphanothece halophytica contains an Na+/H+ antiporter, homologous to eukaryotic ones, with novel ion specificity affected by C-terminal tail. J. Biol. Chem., 276: 36931–36938.
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Kshatriya, K., Singh, J.S. & Singh, D.P. Salt tolerant mutant of Anabaena doliolum exhibiting efficient ammonium uptake and assimilation. Physiol Mol Biol Plants 15, 377–381 (2009). https://doi.org/10.1007/s12298-009-0043-5
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DOI: https://doi.org/10.1007/s12298-009-0043-5