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Garlic Oil and Vitamin E Prevent the Adverse Effects of Lead Acetate and Ethanol Separately as well as in Combination in the Drinking Water of Rats

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Abstract

Daily feeding of drinking water containing lead acetate (160 mg/l) or 10% alcohol by volume or a combination of both to rats for a month produced certain deleterious effects through oxidative stress. Both heavy metal lead and alcohol are capable of doing such damages. The deleterious alterations observed were in the parameters of blood, serum and tissues, viz; Hb, Pb, proteins, lipids, lipid per oxidation, Vitamins C and E levels and enzyme activities of AST, ALT, and catalase. Simultaneous feeding of either of the two antioxidants garlic oil (GO) and vitamin E at equal doses of 100 mg/kg/day, to the rats counteracted the deleterious effects of the above two chemicals significantly. The maximum damage was brought about by feeding of drinking water containing both lead acetate and alcohol. The protective effects of GO and Vitamin E were not significantly different. The mechanism of actions of the Vitamin E and GO is probably due to their efficiency as detoxifying agents and antioxidants, to scavenging free radicals as well as an independent action of GO on the removal of lead salt as lead sulfide.

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References

  1. Juberg DR, Klieman CF, Simons CK. Position paper of the American council on sciences and Health. Lead and human health. Ecotoxicol Environ Safety. 1997;38:162–80.

    Article  CAS  PubMed  Google Scholar 

  2. Goyer RA. Results of lead research. Prenatal exposure and neurological consequences. Environ Health Perspect. 1996;104:1050–4.

    Article  CAS  PubMed  Google Scholar 

  3. Bressler J, Kim KA, Chakraborti T, Goldstein G. Mechanism of lead neurotoxicity. Neurochem Res. 1999;24:595–600.

    Article  CAS  PubMed  Google Scholar 

  4. Flora SJS, Tandon SK. Effect of combined exposure to lead and ethanol on some biochemical indices in the rat. Biochem Pharmacol. 1987;36:537–41.

    Article  CAS  PubMed  Google Scholar 

  5. Flora SJS, Dube SN. Modulatory effects of ethanol ingestion on the toxicology of heavy metals. Ind J Pharmacol. 1994;26:240–8.

    CAS  Google Scholar 

  6. Harishekar MB. Studies on alcohol-lead interactive hepatotoxicity. Ph.D.Thesis (submitted), Rajiv Gandhi University of Health Sciences Bangalore, Karnataka. 2003. pp. 77–97.

  7. Pet Kov V, Stoev V, Bakalov D, Petev L. The Bulgarian drug satlal as a remedy for lead intoxication in industrial conditions. Higiena Truda i Profesionaline Zabolevania. 1965;9(4):42.

    CAS  Google Scholar 

  8. Aminu B, Augusti KT, Joseph PK. Hypolipidemic effects of onion oil and garlic oil in ethanol fed rats. Ind J Biochem Biophys. 1984;21:211–3.

    Google Scholar 

  9. Augusti KT, Anuradha, Prabha SP. Nutraceutical effects of garlic oil, its non polar fraction and a ficus flavonoid as compared to Vitamin E in CC14 induced liver damage in rats. Ind J Exp Biol. 2005;43:437–44.

    CAS  Google Scholar 

  10. Augusti KT, Suneesh K, Krishnankutty BK. Prophylactic and curative effects of garlic oil as compared to α tocopherol against isoproterenol induced damages in rats In: Proceedings of the National Seminar on the Evaluation of Nutraceuticals that prevent diseases held at Thalassery Campus of Kannur University on 29th Nov–2nd Dec 2006. pp. 84–96.

  11. Pinon-Lataillade G, Thoreux-Manlay A, Coffigny H. Effect of ingestion and inhalation of lead on the reproductive system and fertility of adult male rats and their progeny. Hum Exp Toxicol. 1993;12:165–72.

    Article  CAS  PubMed  Google Scholar 

  12. Jayaramman J editor. Modified Biuret method. In: Laboratory Manual in Biochemistry. New Delhi: Wiley Eastern Limited; 1981. p. 78.

  13. Chiamori N, Henry R. Total cholesterol. Zlatkis (modified) method. In: Frankel S, Reitman S, Sonnenwirth AC, editors. Gradwohl’s Clinical Laboratory Methods and Diagnosis. Saint Louis: The C.V. Mosby Company; 1963. p. 257–8.

    Google Scholar 

  14. Friedewalt WT, Levy RT, Frederickson DS. Estimation of concentration of LDL in plasma with out use of preparative ultracentrifuge. Clin Chem. 1972;18:499–502.

    Google Scholar 

  15. Warnick RG, Albers JA. A comprehensive evaluation of the heparin-manganese precipitating procedure for estimating high density lipoprotein cholesterol. J Lipid Res. 1978;19:65–79.

    CAS  PubMed  Google Scholar 

  16. Van Handel E, Zilversmit DB. Serum triglycerides micromethod. In: Frankel S, Reitman S, Sonnenwirth AC, editors. Gradwohl’s Clinical Laboratory Methods and Diagnosis. Saint Louis: The CV Mosby Company; 1963. p. 263–4.

    Google Scholar 

  17. Chaudhry K editor. Haemoglobin estimation by cyamethaemoglobin method. In: Biochemical Techniques. New Delhi: Jaypee Brothers Medical publishers; 1989. pp 83–85.

  18. Tandon SK, Singh S, Prasad S. Influence of garlic on the disposition & toxicity of Lead & cadmium in the rat. Pharmaceu Biol. 2001;39:450–4.

    Article  Google Scholar 

  19. Bergmeyer H, Bernet E, Colorimetric method for aspartatate and alanine aminotransferases. In: Varley H, Gowenlock AH, Bell M editor. Practical Clinical Biochemistry. Vol I, 5th ed. London: William Heinemann Medical Books Ltd.; 1980. p. 741742.

  20. Maehly AC, Chance B. The assay of catalase and peroxidase. In: Glick D, editor. Methods Biochemical Analysis, vol. 1. New York: Interscience; 1954. p. 357–60.

    Chapter  Google Scholar 

  21. Terada M, Watanabe Y, Kunitomo M, Hayashi E. Differential rapid analysis of AA and AAS by diphenyl hydrazine method. Anal Biochem. 1978;84:604–6.

    Article  CAS  PubMed  Google Scholar 

  22. Catiganin GL. An HPLC method for the simultaneous determination of retinol and α tocopherol in plasma or serum. Methods Enzymol. 1986;123:214–9.

    Google Scholar 

  23. Niehaus WG Jr, Samuelson B. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem. 1968;6:126–30.

    Article  CAS  PubMed  Google Scholar 

  24. Chiba M, Shinohara A, Matsushita K, Watanaba H, Inaba Y. Indices of lead exposure in blood and urine of lead-exposed workers and concentrations of major and trace elements and activities of SOD, GSH-Px and catalase in their blood. J Exp Med. 1999;178:49–62.

    Google Scholar 

  25. Sandhir R, Julka D, Gill KD. Lipoperoxidative damage on lead treatment in rat brain and its implications on membrane bound enzymes. Pharmacol Toxicol. 1994;74:66–71.

    Article  CAS  PubMed  Google Scholar 

  26. Sandhir R, Gill KD. Effects of lead on lipid peroxidation in liver of rats. Biol Trace Elem Res. 1995;48:91–7.

    Article  CAS  PubMed  Google Scholar 

  27. Lau BHS. Allergies, pollution, and today’s lifestyle. In: Garlic for Health. Wilmot, WI: Lotus Light Publications; 1988. p. 29–35.

  28. Bailey SM, Cunningham CC. Acute and chronic ethanol increases reactive oxygen species generation and decreases viability in fresh, isolated rat hepatocytes. Hepatology. 1998;28:1318–26.

    Article  CAS  PubMed  Google Scholar 

  29. Blomstrand R, Kager L, Lantto O. Status on studies on the ethanol induced decrease of fatty acid oxidation in rats & human liver slices. Life Sci. 1973;13:113–23.

    Article  CAS  PubMed  Google Scholar 

  30. Ding Y, Gonick HC, Vaziri ND. Lead promotes hydroxyl radical generation and lipid peroxidation in cultured aortic endothetial cells. Am J Hypertens. 2001;13:552–5.

    Article  Google Scholar 

  31. Ding Y, Gonick HC, Vaziri ND, Llang KW. Lead induced hypertension III increased hydroxyl radical production. Am J Hypertens. 2001;14:169–73.

    Article  CAS  PubMed  Google Scholar 

  32. Ellwood PC, Yarnell JWG, Oldham PD. Blood pressure and blood lead surveys in Wales. Am J Exp Demiol. 1988;127:942–5.

    Google Scholar 

  33. Barrio E, Rodriguez I, Tome S, Gude F, Quintela F. Liver disease in heavy drinkers with & without alcohol withdrawal syndrome. Alcohol Clin Exp Res. 2003;28(1):131–6.

    Article  Google Scholar 

  34. Baily SM, Patel VB, Young TA. Chronic ethanol consumption alters the glutathione/glutathione peroxidase-1 system and protein oxidation status in rat liver. Alcohol Clin Exp Res. 2001;25:726–33.

    Article  Google Scholar 

  35. Davies KJA. Protein damage and degeneration by oxygen radicals. J Biochem. 1987;262:9895–902.

    CAS  Google Scholar 

  36. Berlett BS, Stadtman ER. Protein oxidation in aging disease and oxidative stress. J Biol Chem. 1997;272(33):2031–6.

    Article  Google Scholar 

  37. Humphreys DJ. Effects of exposure to excessive quantities of lead on animals. Br Vet J. 1991;147:18–30.

    CAS  PubMed  Google Scholar 

  38. Juin YS, Hsien LT. Lipid peroxidation in workers exposed to lead. Arch Environ Health. 1994;49:256–9.

    Article  Google Scholar 

  39. Farant JP, Wigfield DC. Biomonitoring of lead exposure with ALAD activity ratios. Int Arch Occup Environ Health. 1982;51:15–24.

    Article  CAS  PubMed  Google Scholar 

  40. Hermes-Lima M. How do Ca2+ and 5-aminolevulinic acid derived oxyradical promote injury to isolated mitochondria. Free Radic Biol Med. 1995;19:381–90.

    Article  CAS  PubMed  Google Scholar 

  41. Adonaylo VN, Otieza PI. Pb2+ promotes lipid peroxidation and alteration in membrane physical properties. Toxicology. 1999;132:19–32.

    Article  CAS  PubMed  Google Scholar 

  42. Pande M, Mehta A, Pant BP, Flora SJS. Combined administration of a chelating agent and an antioxidant in the prevention and treatment of acute lead intoxication in rats. Environ Toxicol Pharmacol. 2001;9:173–84.

    Article  CAS  PubMed  Google Scholar 

  43. Gurer H, Ercal N. Can antioxidant be beneficial in the treatment of lead poisoning? Free Radic Biol Med. 2000;29:927–45.

    Article  CAS  PubMed  Google Scholar 

  44. Lawton L, Donaldson WE. Lead induced tissue fatty acid alterations and lipid peroxidation. Biol Terace Elem Res. 1991;28:83–97.

    Article  CAS  Google Scholar 

  45. Fatma M, El-Demerdash FM. Antioxidant effect of Vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol. 2004;18:113–22.

    Article  Google Scholar 

  46. Buettner GR. The packing order of free radicals and antioxidants. Lipid peoxidation α-tocopherol and ascorbate. Arch Biochem Biophys. 1993;300:535–43.

    Article  CAS  PubMed  Google Scholar 

  47. Patra RC, Swarup D, Dwivedi SK. Antioxidant effects of α-tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver, kidney and brain in rats. Toxicology. 2001;162:81–8.

    Article  CAS  PubMed  Google Scholar 

  48. May JM, Qu ZC, Mendiratta S. Protection and recycling of alpha tocopherol in human erythrocytes and intracellular ascorbic acid. Arch Biochem Biophys. 1998;349:281–9.

    Article  CAS  PubMed  Google Scholar 

  49. Klanns-Dieter A. Sulfur content of free radicals. In: Nygaard OF, Simic MG, editors. Radioprotectors and Anticarcinogenes. New York: Academic Press; 1983. p. 23–42.

    Google Scholar 

  50. El-Demerdash FM, Yousef MI, Abou El-Naga NI. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan—induced diabetic rats. Food and Chem Technol. 2005;43(1):57–63.

    Article  CAS  Google Scholar 

  51. Nuutila AM, Pimia RP, Arni M, Caldentey KMO. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem. 2003;81(4):485–93.

    Article  CAS  Google Scholar 

  52. Onyeze GO. Effect of Vitamin E on mono sodium glutamate induced hepato toxicity, oxidative stress in rats. Ind J Biochem Biophys. 2006;43:20–4.

    Google Scholar 

  53. Babalola OO, Ojo LO, Aderemi MO. Lead levels in some biological samples of auto-mechanic in Abeokutta, Nigeria. Ind J Biochem Biophys. 2005;42:401–3.

    CAS  Google Scholar 

  54. Lau BHS. Suppression of LDL oxidation by garlic. J Nutr. 2001;131:958S–88S.

    Google Scholar 

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Acknowledgments

The authors acknowledge with thanks Sree Mookambika Institute of Medical Sciences and the M.G. University authorities for some financial support for the work.

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Authors and Affiliations

  1. Department of Biochemistry, Sree Mookambika Institute of Medical Sciences, Kanyakumari Dist, Kulasekharam, 629161, TN, India

    G. R. Sajitha

  2. Department of Community Medicine, Dr. SMCSI Medical College, Karakonam, Trivandrum, India

    Regi Jose

  3. Department of Medical Biochemistry, School of Medical Education, MG University Centre, Gandhi Nagar, Kottayam, 686 008, India

    A. Andrews & K. G. Ajantha

  4. Department of Surgical Oncology, Regional Cancer Center, Trivandrum, India

    Paul Augustine

  5. Department of Biochemistry (Rtd), Kerala University, Kunnethedam, R1-JaiNagar, Medical College P.O., Thiruvananthapuram, 695011, Kerala, India

    K. T. Augusti

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  1. G. R. Sajitha
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Correspondence to K. T. Augusti.

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Sajitha, G.R., Jose, R., Andrews, A. et al. Garlic Oil and Vitamin E Prevent the Adverse Effects of Lead Acetate and Ethanol Separately as well as in Combination in the Drinking Water of Rats. Indian J Clin Biochem 25, 280–288 (2010). https://doi.org/10.1007/s12291-010-0042-x

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  • DOI: https://doi.org/10.1007/s12291-010-0042-x

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