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Mucibacter soli gen. nov., sp. nov., a new member of the family Chitinophagaceae producing mucin

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Abstract

A Gram-stain-negative, mucus-forming, motile by gliding, non-spore-forming and short rod-shaped bacterial strain designated R1-15T was isolated from soil and its taxonomic position was evaluated using a polyphasic approach. Strain R1-15T grew at 15–37°C (optimum, 30°C), at pH 6–7 (optimum, pH 6) and in the presence of 0–1% (w/v) NaCl (optimum, 0%) on 0.1X TSA. On the basis of 16S rRNA gene sequence similarity, the novel strain was assigned to the family Chitinophagaceae of the phylum Bacteroidetes, and its closest related taxa were species of the genera Taibaiella (88.76–90.02% sequence similarity), Lacibacter (89.24–90.00%), Chitinophaga (88.61–89.76%), and Terrimonas (89.04%). Flexirubin- type pigments were produced. The only isoprenoid quinone was MK-7, and the major polar lipid was phosphatidylethanolamine. Based on whole genome comparisons between the strain R1-15T and the type strains of relatives, the orthologous average nucleotide identity values were 66.9–67.0%. The DNA G + C content of strain R1-15T was 43.8 mol%. The combination of phylogenetic, chemotaxonomic and phenotypic data clearly supported separation of strain R1-15T from related taxa, and thus the name Mucibacter soli gen. nov., sp. nov. is proposed. The type strain is R1-15T (= KCTC 62274T = JCM 31190T).

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Author notes

  1. Min-Kyeong Kim

    Present address: Present address: Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea

  2. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea

    Min-Kyeong Kim, Sewook Park, Tae-Su Kim, Yochan Joung, Ji-Hye Han & Seung Bum Kim

  2. Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, 28159, Republic of Korea

    Sewook Park

  3. Clinical Drug Manufacturing Center, Osong Medical Innovation Foundation, Cheongju, 28160, Republic of Korea

    Tae-Su Kim

  4. Department of Biology, Inha University, Incheon, 22212, Republic of Korea

    Yochan Joung

  5. Bacterial Resources Research Team, Freshwater Bioresources Research Division, Nakdonggang National Institute of Biological Resources, Sangju, 37242, Republic of Korea

    Ji-Hye Han

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  1. Min-Kyeong Kim
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Supplemental material for this article may be found at https://doi.org/www.springerlink.com/content/120956.

The GenBank accession number for the 16S rRNA gene sequence of strain R1-15T is KU497556. The draft genome sequence was deposited to GenBank under the accession number QKTW00000000.

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Kim, MK., Park, S., Kim, TS. et al. Mucibacter soli gen. nov., sp. nov., a new member of the family Chitinophagaceae producing mucin. J Microbiol. 57, 356–361 (2019). https://doi.org/10.1007/s12275-019-8512-1

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