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Pseudaminobacter granuli sp. nov., isolated from granules used in a wastewater treatment plant

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Abstract

A Gram negative, aerobic, non-motile and rod-shaped bacterial strain designated as Gr-2T was isolated from granules used in a wastewater treatment plant in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain Gr-2T grew at 18–37°C (optimum temperature, 30°C) and a pH of 6.0–8.0 (optimum pH, 7.0) on R2A agar medium. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Pseudaminobacter of the family Phyllobacteriaceae, and formed clusters with Pseudaminobacter defluvii THI 051T (98.9%) and Pseudaminobacter salicylatoxidans BN12T (98.7%). The G+C content of the genomic DNA was 63.6%. The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major fatty acids were cyclo-C19:0 ω8c, C18:1 ω7c, and iso-C17:0. The overall polar lipid patterns of Gr-2T were similar to those determined for the other Pseudaminobacter species. DNA-DNA relatedness values between strain Gr-2T and its closest phylogenetically neighbors were below 18%. Strain Gr-2T could be differentiated genotypically and phenotypically from the recognized species of the genus Pseudaminobacter. The isolate therefore represents a novel species, for which the name Pseudaminobacter granuli sp. nov. is proposed with the type strain Gr-2T (=KACC 18877T =LMG 29567T).

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Authors and Affiliations

  1. Samsung Electronics, Seoul, 06620, Republic of Korea

    Young Ki Hahn

  2. Department of New Biology, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu, 42988, Republic of Korea

    Minseok S. Kim

  3. Department of Biotechnology, Hankyong National University, Kyonggi-do, 17579, Republic of Korea

    Wan-Taek Im

  4. Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Kyonggi-do, 17579, Republic of Korea

    Wan-Taek Im

Authors
  1. Young Ki Hahn
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  2. Minseok S. Kim
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  3. Wan-Taek Im
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Correspondence to Minseok S. Kim or Wan-Taek Im.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain Gr-2T is KX066102.

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Hahn, Y.K., Kim, M.S. & Im, WT. Pseudaminobacter granuli sp. nov., isolated from granules used in a wastewater treatment plant. J Microbiol. 55, 607–611 (2017). https://doi.org/10.1007/s12275-017-7257-y

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  • DOI: https://doi.org/10.1007/s12275-017-7257-y

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