Skip to main content
SpringerLink
Log in

Arcobacter acticola sp. nov., isolated from seawater on the East Sea in South Korea

  • Microbial Systematics and Evolutionary Microbiology
  • Published:
Journal of Microbiology Aims and scope Submit manuscript

Abstract

A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13T, was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13T grew optimally at 30°C, at pH 7.0–8.0 and in the presence of 0–0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13T fell within the clade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis. Strain AR-13T exhibited 16S rRNA gene sequence similarity values of 98.1% to the type strain of A. venerupis and of 93.2–96.9% to the type strains of the other Arcobacter species. Strain AR-13T contained MK-6 as the only menaquinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, C18:1 ω7c, and summed feature 2 (iso-C16:1 I and/or C14:0 3-OH) as the major fatty acids. The major polar lipids detected in strain AR-13T were phosphatidylethanolamine, phosphatidylglycerol, and one unidentified aminophospholipid. The DNA G+C content was 28.3 mol% and its mean DNA-DNA relatedness value with the type strain of A. venerupis was 21%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain AR-13T is separated from recognized Arcobacter species. On the basis of the data presented, strain AR-13T is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter acticola sp. nov. is proposed. The type strain is AR-13T (=KCTC 52212T =NBRC 112272T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barrow, G.I. and Feltham, R.K.A. 1993. Cowan and steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge, UK.

    Book  Google Scholar 

  • Baumann, P. and Baumann, L. 1981. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes, pp. 1302–1331. In Starr, M.P., Stolp, H., Trüper, H.G., Balows, A., and Schlegel, H.G. (eds.), The Prokaryotes. Springer-Verlag, Berlin, Germany.

    Google Scholar 

  • Cohen-Bazire, G., Sistrom, W.R., and Stanier, R.Y. 1957. Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J. Cell Comp. Physiol. 49, 25–68.

    Article  CAS  Google Scholar 

  • Collado, L., Levican, A., Perez, J., and Figueras, M.J. 2011. Arcobacter defluvii sp. nov., isolated from sewage samples. Int. J. Syst. Evol. Microbiol. 61, 2155–2161.

    Article  PubMed  Google Scholar 

  • Embley, T.M. and Wait, R. 1994. Structural lipids of eubacteria, pp. 121–161. In Goodfellow, M. and O’Donnell, A.G. (eds.), Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics. John Wiley & Sons, Chichester, England.

    Google Scholar 

  • Ezaki, T., Hashimoto, Y., and Yabuuchi, E. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229.

    Article  Google Scholar 

  • Kim, H.M., Hwang, C.Y., and Cho, B.C. 2010. Arcobacter marinus sp. nov. Int. J. Syst. Evol. Microbiol. 60, 531–536.

    Article  CAS  PubMed  Google Scholar 

  • Komagata, K. and Suzuki, K.I. 1987. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol. 19, 161–207.

    Article  CAS  Google Scholar 

  • Lányí, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19, 1–67.

    Article  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.

    Article  CAS  Google Scholar 

  • Park, S., Jung, Y.T., Park, J.M., and Yoon, J.H. 2014a. Pseudohongiella acticola sp. nov., a novel gammaproteobacterium isolated from seawater, and emended description of the genus Pseudohongiella. Antonie van Leeuwenhoek 106, 809–815.

    Article  CAS  PubMed  Google Scholar 

  • Park, S., Park, D.S., Bae, K.S., and Yoon, J.H. 2014b. Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int. J. Syst. Evol. Microbiol. 64, 1378–1383.

    Article  CAS  PubMed  Google Scholar 

  • Parte, A.C. 2014. LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res. 42, D613–D616.

    Article  CAS  PubMed  Google Scholar 

  • Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI. Inc., Newark, DE, USA.

    Google Scholar 

  • Sasi Jyothsna, T.S., Rahul, K., Ramaprasad, E.V.V., Sasikala, Ch., and Ramana, Ch.V. 2013. Arcobacter anaerophilus sp. nov., isolated from an estuarine sediment and emended description of the genus Arcobacter. Int. J. Syst. Evol. Microbiol. 63, 4619–4625.

    Article  CAS  PubMed  Google Scholar 

  • Stackebrandt, E. and Goebel, B.M. 1994. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44, 846–849.

    Article  CAS  Google Scholar 

  • Staley, J.T. 1968. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J. Bacteriol. 95, 1921–1942.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tamaoka, J. and Komagata, K. 1984. Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25, 125–128.

    Article  CAS  Google Scholar 

  • Vandamme, P., Falsen, E., Rossau, R., Hoste, B., Segers, P., Tytgat, R., and de Ley, J. 1991. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int. J. Syst. Bacteriol. 41, 88–103.

    Article  CAS  PubMed  Google Scholar 

  • Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R., Hommez, J., et al. 1992. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int. J. Syst. Bacteriol. 42, 344–356.

    CAS  PubMed  Google Scholar 

  • Wayne, L.G., Brenner, D.J., Colwell, R.R., Grimont, P.A.D., Kandler, O., Krichevsky, M.I., Moore, L.H., Moore, W.E.C., Murray, R.G.E., Stackebrandt, E., et al. 1987. International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.

    Google Scholar 

  • Yoon, J.H., Kang, K.H., and Park, Y.H. 2003. Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 53, 449–454.

    CAS  PubMed  Google Scholar 

  • Yoon, J.H., Lee, S.T., and Park, Y.H. 1998. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int. J. Syst. Bacteriol. 48, 187–194.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H., Kim, H., Kim, S.B., Kim, H.J., Kim, W.Y., Lee, S.T., Goodfellow, M., and Park, Y.H. 1996. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int. J. Syst. Bacteriol. 46, 502–505.

    Article  CAS  Google Scholar 

  • Zhang, Z., Yu, C., Wang, X., Yu, S., and Zhang, X.H. 2016. Arcobacter pacificus sp. nov., isolated from seawater of the South Pacific Gyre. Int. J. Syst. Evol. Microbiol. 66, 542–547.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea

    Sooyeon Park, Yong-Taek Jung, Sona Kim & Jung-Hoon Yoon

  2. University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

    Yong-Taek Jung

Authors
  1. Sooyeon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-Taek Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sona Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jung-Hoon Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jung-Hoon Yoon.

Additional information

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

Electronic supplementary material

12275_2016_6268_MOESM1_ESM.pdf

Fig. S1. Transmission electron micrograph of the cell of Arcobacter acticola AR-13T from an exponentially growing culture. Bar, 1.0 μm.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Park, S., Jung, YT., Kim, S. et al. Arcobacter acticola sp. nov., isolated from seawater on the East Sea in South Korea. J Microbiol. 54, 655–659 (2016). https://doi.org/10.1007/s12275-016-6268-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-016-6268-4

Keywords

Search

Navigation