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Bacillus daqingensis sp. nov. isolated from near poultry farm soil

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A Publisher’s Erratum to this article was published on 23 July 2014

Abstract

A novel bacterial strain, designated PFS-5T, was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5T was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5T in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5aT (96.9%), B. humi LMG 22167T (96.7%), B. alkalitelluris BA288T (96.1%), B. litoralis SW-211T (96.0%), and B. luteolus YIM93174T (95.5%). The major polar lipids of PFS-5T were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5T represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strai PFS-5T = KACC 17469T = JCM19333T).

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Refrerences

  • Aslam, S.M., Perveez, H., Na, W.H., Khan, H., and Amean, U.M. 2009. Evaluation of biochemical and phytochemical composition of some groundnut varieties grown in Arid zone of Pakistan. Pak. J. Bot. 41, 2739–2749.

    Google Scholar 

  • Bae, S.S., Lee, J.H., and Kim, S.J. 2005. Bacillus alveayuensis sp. nov., a thermophilic bacterium community analysis of Indonesian hot springs. FEMS Microbiol. Lett. 200, 103–109.

    Google Scholar 

  • Claus, D. and Berkeley, C.W. 1986. The genus Bacillus, pp. 1105–1139. In Sneath P.H.A. (ed.), Bergey’s Manual of Systematic Bacteriology, 8th ed. The Williams and Wilkins, Baltimore, USA.

    Google Scholar 

  • Cohn, F. 1872. Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen. 1, 127–224.

    Google Scholar 

  • Euzéby, J.P. 2013. Bacillus Cohn 1872, genus. In List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.cict.fr/b/bacillus.html).

    Google Scholar 

  • Gomori, G. 1995. Preparation of buffers for use in enzyme studies. Methods Enzyme 1, 138–146.

    Article  Google Scholar 

  • Groth, I., Schumann, P., Weiss, N., Martin, K., and Rainey, F.A. 1996. Agrococcus jenensis gen. nov., sp. nov., a new genus of Actinomycetes with diaminobutyric acid in the cell wall. Int. J. Syst. Bacteriol. 46, 234–239.

    Article  CAS  PubMed  Google Scholar 

  • Hall, T.A. 1999. BioEdit: a user-friendly biological Sequence alignment editor and analysis program forWindows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.

    CAS  Google Scholar 

  • Heyrman, J., Rodríguez-Díaz, M., Devos, J., Felske, A., Logan, N.A., and De Vos, P. 2005a. Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 55, 111–117.

    Article  CAS  PubMed  Google Scholar 

  • Heyrman, J., Logan, N.A., Rodríguez-Díaz, M., Scheldeman, P., Lebbe, L., Swings, J., Heyndrickx, M., and De Vos, P. 2005b. Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int. J. Syst. Evol. Microbiol. 55, 119–131.

    Article  CAS  PubMed  Google Scholar 

  • Ivanova, E.P., Alexeeval, Y.A., Zhukova, N.V., Gorshkova, N.M., Buljan, V., Nicolau, D.V., Mikhailov, V.V., and Christen, R. 2004. Bacillus algicola sp., nov., a novel filamentous organism isolated from brown alga Fucus evanescens. System. Appl. Microbiol. 27, 301–307.

    Article  CAS  Google Scholar 

  • Kämpfer, P. 1994. Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst. Appl. Microbiol. 17, 86–98.

    Article  Google Scholar 

  • Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., and et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716–721.

    Article  CAS  PubMed  Google Scholar 

  • Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111–120.

    Article  CAS  PubMed  Google Scholar 

  • Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115–175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics. Wiley, Chichester, UK.

    Google Scholar 

  • Lee, J.C., Lee, G.S., Park, D.J., and Kim, C.J. 2008. Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil. Int. J. Syst. Evol. Microbiol. 58, 2629–2634.

    Article  CAS  PubMed  Google Scholar 

  • Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.

    Article  CAS  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.

    Article  CAS  Google Scholar 

  • Moore, D.D. and Dowhan, D. 1995. Preparation and analysis of DNA, pp. 2–11. In Ausubel, F.W., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.) Current Protocols in Molecular Biology, Wiley, New York, USA.

    Google Scholar 

  • Qiu, F., Zhang, X., Liu, L., Sun, L., Schumann, P., and Song, W. 2009. Bacillus beijingensis sp. nov. and Bacillus ginseng sp. nov., isolated from ginseng root. Int. J. Syst. Evol. Microbiol. 59, 729–734.

    Article  CAS  PubMed  Google Scholar 

  • Skerman, V.B.D. 1967. A Guide to the Identification of the Genera of Bacteria, 2nd (ed.) Williams and Wilkins, Baltimore, USA.

    Google Scholar 

  • Shi, R., Yin, M., Tang, S.K., Lee, J.C., Park. D.J., Zhang, Y.J., Kim, C.J., and Li, W.J. 2011. Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field. Int. J. Syst. Evol. Microbiol. 61, 1344–1349.

    Article  PubMed  Google Scholar 

  • Smibert, R.M. and Krieg, N.R. 1981. General characterization. Manual of Methods for General Bacteriology, pp. 409–443. In Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R., and Phillips, G.B. (eds.). American Society for Microbiology, Washington, D.C., USA.

    Google Scholar 

  • Smibert, R.M. and Krieg, N.R. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology, pp. 617, 620, 622. In Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R. and Phillips, G.B. (eds.) American Society for Microbiology, Washington, D.C., USA.

    Google Scholar 

  • Staneck, J.L. and Roberts, G.D. 1974. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28, 226–231.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA: 5 molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Turnbull, P.C.B. 1996. Bacillus. Barron’s Medical Microbiology 4th (ed.), In Baron, S. (ed.), University of Texas Medical Branch. Galveston, USA.

    Google Scholar 

  • Venkateswaran, K., Kempf, M., Chen, F., Satomi, M., Nicholson, W., and Kern, R. 2003. Bacillus nealsonii sp. nov., isolated from a space craft assembly facility, whose spores are gamma-radiation resistant. Int. J. Syst. Evol. Microbiol. 53, 165–172.

    Article  CAS  PubMed  Google Scholar 

  • Wieser, M., Worliczek, H., Kämpfer, P., and Busse, H.J. 2005. Bacillus herbersteinensis sp. nov. Int. J. Syst. Evol. Microbiol. 55, 2119–2123.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H., Lee, C.H., and Oh, T.K. 2005. Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 55, 733–736.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H., Lee, S.T., and Park, Y.H. 1998. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA sequences. Int. J. Syst. Bacteriol. 48, 187–194.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, J.H. and Oh, T.K. 2005. Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow sea in Korea. Int. J. Syst. Evol. Microbiol. 55, 1945–1948.

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Tae-Young Ahn.

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Kim, HJ., Park, CS., Lee, S. et al. Bacillus daqingensis sp. nov. isolated from near poultry farm soil. J Microbiol. 52, 554–558 (2014). https://doi.org/10.1007/s12275-014-3458-9

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  • DOI: https://doi.org/10.1007/s12275-014-3458-9

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