Abstract
A novel bacterial strain, designated PFS-5T, was isolated from the soil environment with feces of a live poultry farm located in Cheonan, Republic of Korea. Strain PFS-5T was Gram-staining-positive, motile, strictly aerobic bacterium, rod-shaped, and endospore-forming. The strain contained meso-diaminopimelic acid in their peptidoglycan and MK-7 menaquinone. The major fatty acids were anteiso-C15:0 (44.2%), C16:0 (22.2%), and iso-C15:0 (16.7%). The DNA G+C content was 40.1 mol%. Comparative 16S rRNA gene sequence analysis identified strain PFS-5T in the genus Bacillus, exhibiting the highest level of sequence similarity with type strain of B. herbersteinensis D-1,5aT (96.9%), B. humi LMG 22167T (96.7%), B. alkalitelluris BA288T (96.1%), B. litoralis SW-211T (96.0%), and B. luteolus YIM93174T (95.5%). The major polar lipids of PFS-5T were diphosphatidylglycerol and phosphatidylglycerol. On the basis of result from poly-phasic data, strain PFS-5T represents a novel species, for which the name Bacillus cheonanensis sp. nov. is proposed (Type strai PFS-5T = KACC 17469T = JCM19333T).
Similar content being viewed by others
Refrerences
Aslam, S.M., Perveez, H., Na, W.H., Khan, H., and Amean, U.M. 2009. Evaluation of biochemical and phytochemical composition of some groundnut varieties grown in Arid zone of Pakistan. Pak. J. Bot. 41, 2739–2749.
Bae, S.S., Lee, J.H., and Kim, S.J. 2005. Bacillus alveayuensis sp. nov., a thermophilic bacterium community analysis of Indonesian hot springs. FEMS Microbiol. Lett. 200, 103–109.
Claus, D. and Berkeley, C.W. 1986. The genus Bacillus, pp. 1105–1139. In Sneath P.H.A. (ed.), Bergey’s Manual of Systematic Bacteriology, 8th ed. The Williams and Wilkins, Baltimore, USA.
Cohn, F. 1872. Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen. 1, 127–224.
Euzéby, J.P. 2013. Bacillus Cohn 1872, genus. In List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.cict.fr/b/bacillus.html).
Gomori, G. 1995. Preparation of buffers for use in enzyme studies. Methods Enzyme 1, 138–146.
Groth, I., Schumann, P., Weiss, N., Martin, K., and Rainey, F.A. 1996. Agrococcus jenensis gen. nov., sp. nov., a new genus of Actinomycetes with diaminobutyric acid in the cell wall. Int. J. Syst. Bacteriol. 46, 234–239.
Hall, T.A. 1999. BioEdit: a user-friendly biological Sequence alignment editor and analysis program forWindows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.
Heyrman, J., Rodríguez-Díaz, M., Devos, J., Felske, A., Logan, N.A., and De Vos, P. 2005a. Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 55, 111–117.
Heyrman, J., Logan, N.A., Rodríguez-Díaz, M., Scheldeman, P., Lebbe, L., Swings, J., Heyndrickx, M., and De Vos, P. 2005b. Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int. J. Syst. Evol. Microbiol. 55, 119–131.
Ivanova, E.P., Alexeeval, Y.A., Zhukova, N.V., Gorshkova, N.M., Buljan, V., Nicolau, D.V., Mikhailov, V.V., and Christen, R. 2004. Bacillus algicola sp., nov., a novel filamentous organism isolated from brown alga Fucus evanescens. System. Appl. Microbiol. 27, 301–307.
Kämpfer, P. 1994. Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst. Appl. Microbiol. 17, 86–98.
Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., and et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716–721.
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111–120.
Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115–175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics. Wiley, Chichester, UK.
Lee, J.C., Lee, G.S., Park, D.J., and Kim, C.J. 2008. Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil. Int. J. Syst. Evol. Microbiol. 58, 2629–2634.
Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.
Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.
Moore, D.D. and Dowhan, D. 1995. Preparation and analysis of DNA, pp. 2–11. In Ausubel, F.W., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.) Current Protocols in Molecular Biology, Wiley, New York, USA.
Qiu, F., Zhang, X., Liu, L., Sun, L., Schumann, P., and Song, W. 2009. Bacillus beijingensis sp. nov. and Bacillus ginseng sp. nov., isolated from ginseng root. Int. J. Syst. Evol. Microbiol. 59, 729–734.
Skerman, V.B.D. 1967. A Guide to the Identification of the Genera of Bacteria, 2nd (ed.) Williams and Wilkins, Baltimore, USA.
Shi, R., Yin, M., Tang, S.K., Lee, J.C., Park. D.J., Zhang, Y.J., Kim, C.J., and Li, W.J. 2011. Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field. Int. J. Syst. Evol. Microbiol. 61, 1344–1349.
Smibert, R.M. and Krieg, N.R. 1981. General characterization. Manual of Methods for General Bacteriology, pp. 409–443. In Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R., and Phillips, G.B. (eds.). American Society for Microbiology, Washington, D.C., USA.
Smibert, R.M. and Krieg, N.R. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology, pp. 617, 620, 622. In Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R. and Phillips, G.B. (eds.) American Society for Microbiology, Washington, D.C., USA.
Staneck, J.L. and Roberts, G.D. 1974. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28, 226–231.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA: 5 molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.
Turnbull, P.C.B. 1996. Bacillus. Barron’s Medical Microbiology 4th (ed.), In Baron, S. (ed.), University of Texas Medical Branch. Galveston, USA.
Venkateswaran, K., Kempf, M., Chen, F., Satomi, M., Nicholson, W., and Kern, R. 2003. Bacillus nealsonii sp. nov., isolated from a space craft assembly facility, whose spores are gamma-radiation resistant. Int. J. Syst. Evol. Microbiol. 53, 165–172.
Wieser, M., Worliczek, H., Kämpfer, P., and Busse, H.J. 2005. Bacillus herbersteinensis sp. nov. Int. J. Syst. Evol. Microbiol. 55, 2119–2123.
Yoon, J.H., Lee, C.H., and Oh, T.K. 2005. Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 55, 733–736.
Yoon, J.H., Lee, S.T., and Park, Y.H. 1998. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA sequences. Int. J. Syst. Bacteriol. 48, 187–194.
Yoon, J.H. and Oh, T.K. 2005. Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow sea in Korea. Int. J. Syst. Evol. Microbiol. 55, 1945–1948.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supplemental material for this article may be found at http://www.springerlink.com/content/120956
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Kim, HJ., Park, CS., Lee, S. et al. Bacillus daqingensis sp. nov. isolated from near poultry farm soil. J Microbiol. 52, 554–558 (2014). https://doi.org/10.1007/s12275-014-3458-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-014-3458-9