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Functional characterization of Autographa californica multiple nucleopolyhedrovirus ORF43 and phenotypic changes of ORF43-knockout mutant

  • Virology
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Abstract

ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the nucleocapsids were singly enveloped in the polyhedrin matrix while the nucleocapsids of AcMNPV are known to be multiply enveloped. Furthermore, Ac43KO led to a defect in the transcription and expression of polyhedrin, which resulted in reduced occlusion body production. However, Ac43KO did not affect production of budded virus as there was no remarkable difference in budded virus titer. These results suggest that ac43 plays an important role in the expression of polyhedrin, the morphogenesis of occlusion body, and the assembly of virions occluded in occlusion bodies.

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References

  • Ayres, M.D., Howard, S.C., Kuzio, J., Lopez-Ferber, M., and Possee, R.D. 1994. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586–605.

    Article  PubMed  CAS  Google Scholar 

  • Blissard, G.W. 1996. Baculovirus-insect cell interactions. Cytotechnology 20, 73–93.

    Article  PubMed  CAS  Google Scholar 

  • Braunagel, S.C. and Summers, M.D. 1994. Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid profiles. Virology 202, 315–328.

    Article  PubMed  CAS  Google Scholar 

  • Carstens, E.B., Krebs, A., and Gallerneault, C.E. 1986. Identification of an amino acid essential to the normal assembly of Autographa californica nuclear polyhedrosis virus polyhedra. J. Virol. 58, 684–688.

    PubMed  CAS  Google Scholar 

  • Carstens, E.B., Williams, G.V., Faulkner, P., and Partington, S. 1992. Analysis of polyhedra morphology mutants of Autographa californica nuclear polyhedrosis virus: molecular and ultrastructural features. J. Gen. Virol. 73, 1471–1479.

    Article  PubMed  CAS  Google Scholar 

  • Choi, J.Y., Roh, J.Y., Kang, J.N., Shim, H.J., Woo, S.D., Jin, B.R., Li, M.S., and Je, Y.H. 2005. Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system. Biochem. Biophys. Res. Commun. 332, 487–493.

    Article  PubMed  CAS  Google Scholar 

  • Federici, B.A. 1997. Baculovirus pathogenesis, pp. 33–56. In Miller, L.K. (ed.), The baculoviruses, Plenum Publishing Corporation, New York, N.Y., USA.

    Chapter  Google Scholar 

  • Friesen, P.D. 1997. Regulation of baculovirus early gene expression, pp. 141–166. In Miller, L.K. (ed.), The baculovirus, Plenum Publishing Corporation, New York, N.Y., USA.

    Chapter  Google Scholar 

  • Herniou, E.A., Olszewski, J.A., Cory, J.S., and O’Reilly, D.R. 2003. The genome sequence and evolution of baculoviruses. Annu. Rev. Entomol. 48, 211–234.

    Article  PubMed  CAS  Google Scholar 

  • Hu, Z., Luijckx, T., van Dinten, L.C., van Oers, M.M., Hajos, J.P., Bianchi, F.J., van Lent, J.W., Zuidema, D., and Vlak, J.M. 1999. Specificity of polyhedrin in the generation of baculovirus occlusion bodies. J. Gen. Virol. 80, 1045–1053.

    PubMed  CAS  Google Scholar 

  • Jakubowska, A.K., Peters, S.A., Ziemnicka, J., Vlak, J.M., and van Oers, M.M. 2006. Genome sequence of an enhancin gene-rich nucleopolyhedrovirus (NPV) from Agrotis segetum: collinearity with Spodoptera exigua multiple NPV. J. Gen. Virol. 87, 537–551.

    Article  PubMed  CAS  Google Scholar 

  • Jehle, J.A., Blissard, G.W., Bonning, B.C., Cory, J.S., Herniou, E.A., Rohrmann, G.F., Theilmann, D.A., Thiem, S.M., and Vlak, J.M. 2006. On the classification and nomenclature of baculoviruses: A proposal for revision. Arch. Virol. 151, 1257–1266.

    Article  PubMed  CAS  Google Scholar 

  • Ji, X.Y., Sutton, G., Evans, G., Axford, D., Owen, R., and Stuart, D.I. 2010. How baculovirus polyhedra fit square pegs into round holes to robustly package viruses. Embo. J. 29, 505–514.

    Article  PubMed  CAS  Google Scholar 

  • Katsuma, S. and Shimada, T. 2009. Bombyx mori nucleopolyhedrovirus ORF34 is required for efficient transcription of late and very late genes. Virology 392, 230–237.

    Article  PubMed  CAS  Google Scholar 

  • Lin, G.Y., Zhong, J., and Wang, X.Z. 2000. Abnormal formation of polyhedra resulting from a single mutation in the polyhedrin gene of Autographa californica multicapsid nucleopolyhedrovirus. J. Invertebr. Pathol. 76, 13–19.

    Article  PubMed  CAS  Google Scholar 

  • Lopez, M.G., Alfonso, V., Carrillo, E., and Taboga, O. 2011. Description of a novel single mutation in the AcMNPV polyhedrin gene that results in abnormally large cubic polyhedra. Arch. Virol. 156, 695–699.

    Article  PubMed  CAS  Google Scholar 

  • Lu, A., Krell, P., Vlak, J.M., and Rohrmann, G.F. 1997. Baculovirus DNA replication, pp. 171–192. In Miller, L.K. (ed.), The baculovirus, Plenum Publishing Corporation, New York, N.Y., USA.

    Chapter  Google Scholar 

  • McCarthy, C.B., Dai, X., Donly, C., and Theilmann, D.A. 2008. Autographa californica multiple nucleopolyhedrovirus ac142, a core gene that is essential for BV production and ODV envelopment. Virology 372, 325–339.

    Article  PubMed  CAS  Google Scholar 

  • Monsma, S.A., Oomens, A.G., and Blissard, G.W. 1996. The gp64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection. J. Virol. 70, 4607–4616.

    PubMed  CAS  Google Scholar 

  • O’Reilly, D.R., Miller, L.K., and Luckow, V.A. 1992. Baculovirus expression vector: A laboratory manual. W.H. Freeman & Company, New York, N.Y., USA.

    Google Scholar 

  • Pfaffl, M.W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45.

    Article  PubMed  CAS  Google Scholar 

  • Ribeiro, B.M., Generino, A.P., Acacio, C.N., Kalapothakis, E., and Bao, S.N. 2009. Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutant. Virus Res. 140, 1–7.

    Article  PubMed  CAS  Google Scholar 

  • Simon, O., Williams, T., Asensio, A.C., Ros, S., Gaya, A., Caballero, P., and Possee, R.D. 2008. Sf29 gene of Spodoptera frugiperda multiple nucleopolyhedrovirus is a viral factor that determines the number of virions in occlusion bodies. J. Virol. 82, 7897–7904.

    Article  PubMed  CAS  Google Scholar 

  • Volkman, L.E. 1997. Nucleopolyhedrovirus interactions with their insect hosts. Adv. Virus Res. 48, 313–348.

    Article  PubMed  CAS  Google Scholar 

  • Wang, L., Salem, T.Z., Campbell, D.J., Turney, C.M., Kumar, C.M., and Cheng, X.W. 2009. Characterization of a virion occlusion-defective Autographa californica multiple nucleopolyhedrovirus mutant lacking the p26, p10 and p74 genes. J. Gen. Virol. 90, 1641–1648.

    Article  PubMed  CAS  Google Scholar 

  • Washburn, J.O., Chan, E.Y., Volkman, L.E., Aumiller, J.J., and Jarvis, D.L. 2003. Early synthesis of budded virus envelope fusion protein GP64 enhances Autographa californica multicapsid nucleopolyhedrovirus virulence in orally infected Heliothis virescens. J. Virol. 77, 280–290.

    Article  PubMed  CAS  Google Scholar 

  • Washburn, J.O., Lyons, E.H., Haas-Stapleton, E.J., and Volkman, L.E. 1999. Multiple nucleocapsid packaging of Autographa californica nucleopolyhedrovirus accelerates the onset of systemic infection in Trichoplusia ni. J. Virol. 73, 411–416.

    PubMed  CAS  Google Scholar 

  • Williams, G.V. and Faulkner, P. 1997. Cytological changes and viral morphogenesis during baculovirus infection, pp. 61–108. In Miller, L.K. (ed.), The baculovirus, Plenum Publishing Corporation, New York, N.Y., USA.

    Chapter  Google Scholar 

  • Woo, S.D., Kim, W.J., Kim, H.S., Jin, B.R., Lee, Y.H., and Kang, S.K. 1998. The morphology of the polyhedra of a host range-expanded recombinant baculovirus and its parents. Arch. Virol. 143, 1209–1214.

    Article  PubMed  CAS  Google Scholar 

  • Wu, W. and Passarelli, A.L. 2010. Autographa californica multiple nucleopolyhedrovirus Ac92 (ORF92, P33) is required for budded virus production and multiply enveloped occlusion-derived virus formation. J. Virol. 84, 12351–12361.

    Article  PubMed  CAS  Google Scholar 

  • Xue, J.L., Salem, T.Z., Turney, C.M., and Cheng, X.W. 2010. Strategy of the use of 28S rRNA as a housekeeping gene in real-time quantitative PCR analysis of gene transcription in insect cells infected by viruses. J. Virol. Methods 163, 210–215.

    Article  PubMed  CAS  Google Scholar 

  • Yu, I.L., Bray, D., Lin, Y.C., and Lung, O. 2009. Autographa californica multiple nucleopolyhedrovirus ORF 23 null mutant produces occlusion-derived virions with fewer nucleocapsids. J. Gen. Virol. 90, 1499–1504.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University, Seoul, 151-742, Republic of Korea

    Xue Ying Tao, Joo Hyun Lee, Qin Liu & Yeon Ho Je

  2. School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou, 730000, P. R. China

    Yong Wang

  3. Division of Medical Entomology, Korea National Institute of Health, Chungbuk, 363-951, Republic of Korea

    Jong Yul Roh

  4. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, Republic of Korea

    Jae Young Choi, Jong Bin Park, Woojin Kim & Yeon Ho Je

  5. Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Jae Su Kim

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  1. Xue Ying Tao
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  2. Jae Young Choi
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  10. Yeon Ho Je
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Correspondence to Yeon Ho Je.

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Tao, X.Y., Choi, J.Y., Wang, Y. et al. Functional characterization of Autographa californica multiple nucleopolyhedrovirus ORF43 and phenotypic changes of ORF43-knockout mutant. J Microbiol. 51, 515–521 (2013). https://doi.org/10.1007/s12275-013-3058-0

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  • DOI: https://doi.org/10.1007/s12275-013-3058-0

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