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Phenotypic and genotypic analysis of clarithromycin-resistant Helicobacter pylori from Bogotá D.C., Colombia

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Resistance of Helicobacter pylori to clarithromycin is the most common cause of treatment failure in patients with H. pylori infections. This study describes the MICs and the presence of 23S rRNA mutations of H. pylori isolates from Bogotá, D.C., Colombia. H. pylori were isolated from gastric biopsies from patients with functional dyspepsia. Clarithromycin susceptibility was investigated by agar dilution and strains were considered resistant if the MIC was ≥1 μg/ml. DNA sequences of the 23S rRNA gene of strains resistant and sensitive to clarithromycin were determined to identify specific point mutations. Clarithromycin resistance was present in 13.6% of patients by agar dilution. The A2143G, A2142G and A2142C mutations were found in 90.5, 7.1, and 2.4% of H. pylori strains with resistance genotype.The resistant phenotype was associated with 23S rRNA resistance genotype in 85.7% of isolates. The point mutations in 23S rRNA were well correlated with MICs values for clarithromycin.

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Authors and Affiliations

  1. Infectious Diseases Group, Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia

    Alba A. Trespalacios, Marcela M. Mercado, Jenny Ávila, Liliana E. Rosero & Azucena Arévalo

  2. Gastroenterology Unit. Clínica Fundadores, Bogotá, D.C., Colombia

    William Otero

  3. Universidad Nacional de Colombia, Bogotá, D.C., Colombia

    William Otero

  4. Biochemistry Unit, Universidad Nacional de Colombia, Bogotá, D.C., Colombia

    Jorge E. Caminos

  5. Molecular Biotechnology Laboratory, Environment and Industrial Biotechnology Group (GBAI), Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia

    Raúl A. Poutou-Piñales

  6. Department of Medicine, Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX, USA

    David Y. Graham

  7. Baylor College of Medicine, Houston, TX, USA

    David Y. Graham

Authors
  1. Alba A. Trespalacios
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  2. William Otero
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  3. Jorge E. Caminos
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  4. Marcela M. Mercado
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  5. Jenny Ávila
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  6. Liliana E. Rosero
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  7. Azucena Arévalo
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  8. Raúl A. Poutou-Piñales
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  9. David Y. Graham
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Correspondence to Alba A. Trespalacios.

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Trespalacios, A.A., Otero, W., Caminos, J.E. et al. Phenotypic and genotypic analysis of clarithromycin-resistant Helicobacter pylori from Bogotá D.C., Colombia. J Microbiol. 51, 448–452 (2013). https://doi.org/10.1007/s12275-013-2465-6

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  • DOI: https://doi.org/10.1007/s12275-013-2465-6

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