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Diversity analysis of Burkholderia cepacia complex in the water bodies of West Lake, Hangzhou, China

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Abstract

A survey of Burkholderia cepacia complex (Bcc) species was conducted in water bodies of West Lake in China. A total of 670 bacterial isolates were recovered on selective media. Out of them, 39.6% (265 isolates) were assigned to the following species: Burkholderia multivorans, Burkholderia cenocepacia recA lineage IIIA, IIIB, Burkholderia stabilis, Burkholderia vietnamiensis, and Burkholderia seminalis while B. cenocepacia is documented as a dominant Bcc species in water of West Lake. In addition, all Bcc isolates tested were PCR negative for the cblA and esmR transmissibility marker genes except B. cenocepacia IIIB A8 which was positive for esmR genelater. The present study raises great concerns on the role of West Lake as a “reservoir” for potential Bcc pathogenic strains.

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References

  • American Public Health Association, American Water Works Association and Water Environment Federation, 1998. Standard Methods for the Examination of Water and Wastewater, 20th ed. American Public Health Association, Washington, DC, USA.

    Google Scholar 

  • Baldwin, A., E. Mahenthiralingam, P. Drevinek, C. Pope, D.J. Waine, D.A. Henry, D.P. Speert, and et al. 2008. Elucidating global epidemiology of Burkholderia multivorans in cases of cystic fibrosis by multilocus sequence typing. J. Clin. Microbiol. 46, 290–295.

    Article  PubMed  CAS  Google Scholar 

  • Baldwin, A., E. Mahenthiralingam, P. Drevinek, P. Vandamme, J.R. Govan, D.J. Wane, J.J. LiPuma, and et al. 2007. Environmental Burkholderia cepacia complex isolates in human infections. Emerg. Infect. Dis. 13, 458–461.

    Article  PubMed  CAS  Google Scholar 

  • Burkholder, W.H. 1950. Sour skin, a bacterial rot of onion bulbs. Phytopathology 40, 115–117.

    Google Scholar 

  • Clode, F.E., M.E. Kaufmann, H. Malnick, and T.L. Pitt. 2000. Distribution of genes encoding putative transmissibility factors among epidemic and nonepidemic strains of Burkholder ia cepacia from cystic fibrosis patients in the United Kingdom. J. Clin. Microbiol. 38, 1763–1766.

    PubMed  CAS  Google Scholar 

  • Coenye, T. and P. Vandamme. 2003. Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ. Microbiol. 5, 719–729.

    Article  PubMed  CAS  Google Scholar 

  • Dalmastri, C., L. Pirone, S. Tabacchioni, A. Bevivino, and L. Chiarini. 2005. Efficacy of species-specific recA PCR tests in the identification of Burkholderia cepacia complex environmental isolates. FEMS Microbiol. Lett. 246, 39–45.

    Article  PubMed  CAS  Google Scholar 

  • Fang, Y., M.M. Lou, B. Li, G. Xie, F. Wang, L. Zhang, and Y.C. Luo. 2010. Characterization of Burkholderia cepacia complex from cystic fibrosis patients in China and their chitosan susceptibility. World J. Microbiol. Biotechnol. 26, 443–450.

    Article  CAS  Google Scholar 

  • Fera, M.T., T.L. Maugeri, C. Gugliandolo, C. Beninati, M.E. Giannone LaCamera, and M. Carbone. 2004. Detection of Arcobacter spp. in the coastal environment of the Mediterranean Seasea. Appl. Environ. Microbiol. 70, 1271–1276.

    Article  PubMed  CAS  Google Scholar 

  • Govan, J.R. and V. Deretic. 1996. Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60, 539–574.

    PubMed  CAS  Google Scholar 

  • Jin, S., G.L. Wang, Q.S. Zhao, H.Q. Chen, and Y.E. Chen. 2005. Preliminary studies on pathogen and hemopathology of the hite cloud disease in Micropte snlmoide. Acta Hydrobiologica Sinica 29, 184–188. (Chinese journal with English abstract)

    CAS  Google Scholar 

  • LiPuma, J.J., T. Spilker, T. Coenye, and C.F. Gonzalez. 2002. An epidemic Burkholderia cepacia complex strain identified in soil. Lancet 359, 2002–2003.

    Article  PubMed  Google Scholar 

  • Mahenthiralingam, E., A. Baldwin, and C.G. Dowson. 2008. Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. J. Appl. Microbiol. 104, 1539–1551.

    Article  PubMed  CAS  Google Scholar 

  • Mahenthiralingam, E., J. Bischof, S.K. Byrne, C. Radomski, J.E. Davies, Y. Av-Gay, and P. Vandamme. 2000. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burk holderia stabilis, Burkholderia cepacia genomovars I and III. J. Clin. Microbiol. 38, 3165–3173.

    PubMed  CAS  Google Scholar 

  • Mahenthiralingam, E., D.A. Simpson, and D.P. Speert. 1997. Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J. Clin. Microbiol. 35, 808–816.

    PubMed  CAS  Google Scholar 

  • McDowell, A., E. Mahenthiralingam, K.E.A. Dunbar, J.E. Moore, M. Crowe, and S.J. Elborn. 2004. Epidemiology of Burkholderia cepacia complex species recovered from cystic fibrosis patients: issues related to patient segregation. J. Med. Microbiol. 53, 663–668.

    Article  PubMed  CAS  Google Scholar 

  • Petrucca, A., P. Cipriani, P. Valenti, D. Santapaola, C. Cimmino, G.L. Scoarughi, I. Santino, S. Stefani, R. Sessa, and M. Nicoletti. 2003. Molecular characterization of Burkholderia cepacia isolates from cystic fibrosis (CF) patients in an Italian CF center. Res. Microbiol. 154, 491–498.

    Article  PubMed  CAS  Google Scholar 

  • Pirone, L., L. Chiarini, C. Dalmastri, A. Bevivino, and S. Tabacchioni. 2005. Detection of cultured and uncultured Burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere. Environ. Microbiol. 7, 1734–1742.

    Article  PubMed  CAS  Google Scholar 

  • Ramette, A., J.J. LiPuma, and J.M. Tiedje. 2005. Species abundance and diversity of Burkholderia cepacia complex in the environment. Appl. Environ. Microbiol. 71, 1193–1201.

    Article  PubMed  CAS  Google Scholar 

  • Vandamme, P., D. Henry, T. Coeyne, S. Nuzla, M. Vancanneyt, J.J LiPuma, D.P. Speert, J.R.W. Govan, and E. Mahenthiralingam. 2002. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia: two additional Burkholderia cepacia complex bacteria may confound results of new molecular diagnostic tools. FEMS Immunol. Med. Microbiol. 33, 143–149.

    Article  PubMed  CAS  Google Scholar 

  • Vanlaere, E., A. Baldwin, D. Gevers, D. Henry, E.D. Brandt, J.J. LiPuma, E. Mahenthiralingam, D.P. Speert, C. Dowson, and P. Vandamme. 2009. Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species: Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov. Int. J. Syst. Evol. Microbiol. 59, 102–111.

    Article  PubMed  CAS  Google Scholar 

  • Vanlaere, E., J.J. LiPuma, A. Baldwin, D. Henry, E.D. Brandt, E. Mahenthiralingam, D.P. Speert, C. Dowson, and P. Vandamme. 2008. Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evol. Microbiol. 58, 1580–1590.

    Article  PubMed  CAS  Google Scholar 

  • Vermis, K., M. Brachkova, P. Vandamme, and H. Nelis. 2003. Isolation of Burkholderia cepacia complex genomovars from waters. Syst. Appl. Microbiol. 26, 595–600.

    Article  PubMed  CAS  Google Scholar 

  • Vermis, K., T. Coenye, E. Mahenthiralingam, H.J. Nelis, and P. Vandamme. 2002. Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex. J. Med. Microbiol. 51, 937–940.

    PubMed  CAS  Google Scholar 

  • Waine, D.J., D.A. Henry, A. Baldwin, D.P. Speert, D. Honeybourne, E. Mahenthiralingam, and C.G. Dowson. 2007. Reliability of multilocus sequence typing of the Burkholderia cepacia complex in cystic fibrosis. J. Cyst. Fibros. 6, 215–219.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, L.X. and G.L. Xie. 2007. Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize. FEMS Microbiol. Lett. 266, 231–235.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, P. R. China

    Yuan Fang, Guan-lin Xie, Miao-miao Lou, Bin Li & Ibrahim Muhammad

  2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, P. R. China

    Yuan Fang

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  1. Yuan Fang
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  2. Guan-lin Xie
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  3. Miao-miao Lou
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  4. Bin Li
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  5. Ibrahim Muhammad
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Correspondence to Guan-lin Xie.

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Fang, Y., Xie, Gl., Lou, Mm. et al. Diversity analysis of Burkholderia cepacia complex in the water bodies of West Lake, Hangzhou, China. J Microbiol. 49, 309–314 (2011). https://doi.org/10.1007/s12275-011-0267-2

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  • DOI: https://doi.org/10.1007/s12275-011-0267-2

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