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Purification and characterization of the α-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756

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Abstract

An α-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70°C. Enzyme showed stability stable in the pH range of 3.0–9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60°C. In the presence of ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ this enzyme maintained 90–105% of its maximum activity and was inhibited by Cr3+, Ag+, and Hg2+. The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and α-PNPG. The Km measured for the α-glucosidase was 0.07 μM, with a Vmax of 318.0 μmol/min/mg.

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  1. Laboratory of Bichemistry and Applied Microbiology, São Paulo State University-Unesp, São José do Rio Preto, 15054-000, Brazil

    Ana Flávia Azevedo Carvalho, Maurício Boscolo, Roberto da Silva, Henrique Ferreira & Eleni Gomes

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  1. Ana Flávia Azevedo Carvalho
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  2. Maurício Boscolo
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  3. Roberto da Silva
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  4. Henrique Ferreira
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Correspondence to Ana Flávia Azevedo Carvalho.

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Carvalho, A.F.A., Boscolo, M., da Silva, R. et al. Purification and characterization of the α-glucosidase produced by thermophilic fungus Thermoascus aurantiacus CBMAI 756. J Microbiol. 48, 452–459 (2010). https://doi.org/10.1007/s12275-010-9319-2

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