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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01

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Abstract

Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).

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Authors and Affiliations

  1. Industrial Bio-materials Research Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Do Young Kim & Ho-Yong Park

  2. Biological Resources Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Doo-Sang Park & Kyung Sook Bae

  3. Department of Microbiology, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Soon Bum Kwon, Moon Gyu Chung & Young Ha Rhee

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  1. Do Young Kim
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  2. Doo-Sang Park
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  3. Soon Bum Kwon
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  4. Moon Gyu Chung
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  5. Kyung Sook Bae
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  6. Ho-Yong Park
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  7. Young Ha Rhee
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Correspondence to Young Ha Rhee.

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Kim, D.Y., Park, DS., Kwon, S.B. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01. J Microbiol. 47, 651–656 (2009). https://doi.org/10.1007/s12275-009-0109-7

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  • DOI: https://doi.org/10.1007/s12275-009-0109-7

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