Abstract
Plasmid pRJ6 is the first known bacteriocinogenic mobilizable (Mob) plasmid of Staphylococcus aureus. Its Mob region is composed of four mob genes (mobCDAB) arranged as an operon, a genetic organization uncommon among S. aureus Mob plasmids. oriT pRJ6 was detected in a region of 431 bp, positioned immediately upstream of mobC. This region, when cloned into pCN37, was able to confer mobilization to the re-combinant plasmid only in the presence of pRJ6. The entire Mob region, including oriT pRJ6, is much more similar to Mob regions from several coagulase-negative staphylococci plasmids, although some remarkable similarities with S. aureus Mob plasmids can also be noted. These similarities include the presence within oriT pRJ6 of the three mcb (MobC binding sites), firstly described in pC221 and pC223, an identical nick site also found in these same plasmids, and a nearly identical sra pC223 site (sequence recognized by MobA). pRJ6 was successfully transferred to S. epidermidis by conjugation in the presence of the conjugative plasmid pGOl. Altogether these findings suggest that pRJ6 might have been originally a coagulase-negative staphylococci plasmid that had been transferred successfully to S. aureus.
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References
Abajy, M.Y., J. Kopeć, K. Schiwon, M. Burzynski, M. Döring, C. Bohn, and E. Grohmann. 2007. A type IV-secretion-like system is required for conjugative DNA transport of broad-host-range plasmid pIP501 in gram-positive bacteria. J. Bacteriol. 189, 2487–2496.
Apisiridej, S., A. Leelaporn, C.D. Scaramuzzi, R.A. Skurray, and N. Firth. 1997. Molecular analysis of a mobilizable theta-mode trimethoprim resistance plasmid from coagulase-negative staphylococci. Plasmid 38, 13–24.
Backert, S. and T.F. Meyer. 2006. Type IV secretion systems and their effectors in bacterial pathogenesis. Curr. Opin. Microbiol. 9, 207–217.
Bastos, M.C.F., M.C. Bonaldo, and E.G.C. Penido. 1980. Constitutive erythromycin resistance plasmid in Staphylococcus aureus. J. Gen. Microbiol. 121, 513–516.
Caryl, J.A. and C.D. Thomas. 2006. Investigating the basis of substrate recognition in the pC221 relaxosome. Mol. Microbiol. 60, 1302–1318.
Charpentier, E., A.I. Anton, P. Barry, B. Alfonso, Y. Fang, and R.P. Novick. 2004. Novel cassette-based shuttle vector system for Gram-positive bacteria. Appl. Environ. Microbiol. 70, 6076–6085.
Francia, M.V., A. Varsaki, M.P. Garcillán-Barcia, A. Latorre, C. Drainas, and F. De La Cruz. 2004. A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol. Rev. 28, 79–100.
Giambiagi-deMarval, M., M.A. Mafra, E.G.C. Penido, and M.C.F. Bastos. 1990. Distinct groups of plasmids correlated with bacteriocin production in Staphylococcus aureus. J. Gen. Microbiol. 136, 1591–1599.
Grohmann, E., G. Muth, and M. Espinosa. 2003. Conjugative plasmid transfer in Gram-positive bacteria. Microbiol. Mol. Biol. Rev. 67, 277–301.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.
Llosa, M., X. Gomis-Rüth, M. Coll, and F. De La Cruz. 2002. Bacterial conjugation: a two-step model for DNA transport. Mol. Microbiol. 45, 1–8.
Ma, J., A. Campbell, and S. Karlin. 2002. Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures. J. Bacteriol. 184, 5733–5745.
Nascimento, J.S., P.C. Fagundes, M.A.P. Brito, K.R.N. Santos, and M.C.F. Bastos. 2005a. Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis. Vet. Microbiol. 106, 61–71.
Nascimento, J.S., M. Giambiagi-deMarval, S.S. Oliveira, H. Ceotto, K.R.N. Santos, and M.C.F. Bastos. 2005b. Genomic fingerprinting of bacteriocin-producer strains of Staphylococcus aureus. Res. Microbiol. 156, 837–842.
Nascimento, J.S., K.R.N. Santos, E. Gentilini, D. Sordelli, and M.C.F. Bastos. 2002. Phenotypic and genetic characterisation of bacteriocin-producer strains of Staphylococcus aureus involved in bovine mastitis. Vet. Microbiol. 85, 133–144.
Netz, D.J.A., H.G. Sahl, R. Marcolino, J.S. Nascimento, S.S. Oliveira, M.B. Soares, and M.C.F. Bastos. 2001. Molecular characterisation of aureocin A70, a multiple-peptide bacteriocin isolated from Staphylococcus aureus. J. Mol. Biol. 311, 939–949.
Nomura, N., M. Yamashita, and Y. Murooka. 1996. Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene 170, 57–62.
Novick, R.P. 1967. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33, 155–166.
Oliveira, S.S., J.S. Nascimento, D.C. Póvoa, S.A Araújo, M.R. Gamon, and M.C.F. Bastos. 1998. Genetic analysis of the bacteriocin-encoding plasmids pRJ6 and pRJ9 of Staphylococcus aureus by transposon mutagenesis and cloning of genes involved in bacteriocin production. J. Appl. Microbiol. 85, 792–814.
Pansegrau, W., W. Schröder, and E. Lanka. 1994. Concerned action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of the conjugative plasmid RP4. J. Biol. Chem. 269, 2782–2789.
Perwez, T. and R.J. Meyer. 1996. MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA. J. Bacteriol. 178, 5762–5767.
Projan, S.J. and G.L. Archer. 1989. Mobilization of the relaxable Staphylococcus aureus plasmid pC221 by the conjugative plasmid pGO1 involves three pC221 loci. J. Bacteriol. 171, 1841–1845.
Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York, N.Y., USA.
Schenk, S. and R.A. Laddaga. 1992. Improved method for electroporation of Staphylococcus aureus. FEMS Microbiol. Lett. 73, 133–138.
Shine, J. and L. Dalgarno. 1974. Correlation between the 3′ terminal-polypyrimidine sequence of 16S RNA and translational specificity of the ribosome. Proc. Natl. Acad. Sci. USA 71, 1342–1346.
Smith, M.C.A. and C.D. Thomas. 2004. An accessory protein is required for relaxosome formation by small staphylococcal plasmids. J. Bacteriol. 186, 3363–3373.
Yanisch-Pérron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103–119.
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Varella Coelho, M.L., Ceotto, H., Madureira, D.J. et al. Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus . J Microbiol. 47, 327–336 (2009). https://doi.org/10.1007/s12275-009-0044-7
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DOI: https://doi.org/10.1007/s12275-009-0044-7