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Expression and regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase genes in Mycobacterium sp. strain JC1 DSM 3803

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Abstract

Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme of the Calvin reductive pentose phosphate cycle. Two sets of structural genes (cbbLS-1 and -2) for form I RubisCO have been previously identified in the Mycobacterium sp. strain JC1, which is able to grow on carbon monoxide (CO) or methanol as sole sources of carbon and energy. Northern blot and reverse transcriptase PCR showed that the cbbLS-1 and -2 genes are expressed in cells grown on either carbon monoxide (CO) or methanol, but not in cells grown in nutrient broth. A promoter assay revealed that the cbbLS-2 promoter has a higher activity than the cbbLS-1 promoter in both CO- and methanol-grown cells, and that the activities of both promoters were higher in CO-grown cells than in methanol-grown cells. A gel mobility shift assay and footprinting assays showed that CbbR expressed in Escherichia coli from a cbbR gene, which is located downstream of cbbLS-1 and transcribed in the same orientation as that of the cbbLS genes, specifically bound to the promoter regions of the cbbLS-1 and -2 genes containing inverted repeat sequence. A DNase I footprinting assay revealed that CbbR protected positions −59 to −3 and −119 to −78 of the cbbLS-1 and -2 promoters, respectively. Overexpression of CbbR induced the transcription of RubisCO genes in Mycobacterium sp. strain JC1 grown in nutrient broth. Our results suggest that the CbbR product from a single cbbR gene may positively regulate two cbbLS operons in the Mycobacterium sp. strain JC1 as is the case for Rhodobacter sphaeroides and Cupriavidus necator.

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  1. Dong Oh Park

    Present address: #112, Chuncheon Bioindustry Foundation, Hi-tech Venture Town, Chuncheon, 200-957, Republic of Korea

  2. Sae Woong Park

    Present address: Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA

  3. Eun Ha Hwang

    Present address: Magnetic Resonance Team, Korea Basic Science Institute, Choongbuk, 363-883, Republic of Korea

Authors and Affiliations

  1. Department of Biology, Yonsei University, Seoul, 120-749, Republic of Korea

    Jae Ho Lee, Dong Oh Park, Sae Woong Park, Eun Ha Hwang & Young Min Kim

  2. Department of Microbiology, Pusan National University, Busan, 609-735, Republic of Korea

    Jeong Il Oh

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Lee, J.H., Park, D.O., Park, S.W. et al. Expression and regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase genes in Mycobacterium sp. strain JC1 DSM 3803. J Microbiol. 47, 297–307 (2009). https://doi.org/10.1007/s12275-008-0210-3

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