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Characterization of conjugative plasmids carrying antibiotic resistance genes encoding 16S rRNA methylase, extended-spectrum beta-lactamase, and/or plasmid-mediated AmpC beta-lactamase

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Abstract

In this study, we identified extended-spectrum β-lactamase (ESBL) and plasmid-mediated AmpC β-lactamase which were associated with 16S rRNA methylase gene on the conjugative plasmid. Among 82 clinical isolates of Enterobacteriaceae that carry 16S rRNA methylase gene (64 strains, armA, and 18 strains, rmtB), bla SHV-12 was detected either alone or combined with bla DHA-1, bla CTX-M-3, and bla CTX-M-14 in 30 strains carrying armA and 6 strains carrying rmtB. The bla CTX-M-3 was detected in 13 of 64 strains carrying armA but no strains carrying rmtB. Whereas bla CTX-M-14 was detected in 15 of 18 strains carrying rmtB but only 2 of 64 strains carrying armA. Overall, bla SHV-12 and bla CTX-M-14 was the most common ESBL gene which was associated with armA and rmtB, respectively. In addition, we found that bla CTX-M-3 localized with armA on the same IncL/M plasmid and bla CTX-M-14 localized with rmtB on the same IncA/C plasmid. Restriction fragment length polymorphism of conjugative plasmids and pulsed-field gel electrophoresis of genomic DNAs revealed that intercellular horizontal transfer of conjugative plasmid and clonal transmission have been occurred at the same time.

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Authors and Affiliations

  1. Department of Microbiology, Kyungpook National University, School of Medicine, Daegu, 700-422, Republic of Korea

    Hee Young Kang, Jungmin Kim, Sung Yong Seol, Yoo Chul Lee, Je Chul Lee & Dong Taek Cho

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  1. Hee Young Kang
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  2. Jungmin Kim
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  3. Sung Yong Seol
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  4. Yoo Chul Lee
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  5. Je Chul Lee
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  6. Dong Taek Cho
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Correspondence to Jungmin Kim.

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Kang, H.Y., Kim, J., Seol, S.Y. et al. Characterization of conjugative plasmids carrying antibiotic resistance genes encoding 16S rRNA methylase, extended-spectrum beta-lactamase, and/or plasmid-mediated AmpC beta-lactamase. J Microbiol. 47, 68–75 (2009). https://doi.org/10.1007/s12275-008-0158-3

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